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O-25 A gene expression map of Vitis vinifera ‘Corvina’ development M. Fasoli, S. Zenoni, S. Dal Santo, P. Tononi, M. Delledonne, M. Pezzotti* Department of Biotechnology, University of Verona, Verona, Italy *Corresponding author: mario.pezzotti@univr.it Transcriptional programs are important in the development of multicellular organisms. In contrast to most animals, plants develop continuously, with new organs being initiated and elaborated throughout the life cycle of the organism. As a consequence, individuals consist of repeated units, which are present in many developmental stages at any given time of the life cycle. It follows that many transcriptional programs underlying the development of different organ systems are continuously active. We analyzed global gene expression during development of the plant Vitis vinifera ‘Corvina’ in samples covering many stages and different organs. Sampling entailed the collection of 18 organs, split in different stages of development. The bud was collected in four stages, from dormancy to bud burst. The prompt bud splits into three lateral buds to form leaves together with either a tendril or inflorescence. These organs were collected in three stages of development for leaves and tendrils, and four for inflorescences. The flowers were split into anthers, pollen, ovaries, petals and sepals. Berries were sampled during five stages of development. After harvest, when berries undergo withering, sampling was carried out after the first, second and third months of this withering phase. Of the differently developed berries, multiple tissues were extracted (skin, flesh and seed) to further investigate the features of this organ. The rachis was also collected in the same five stages of berry development. Stems were picked in two phases, the green and woody stages of the cane. Furthermore, tissues were collected in order to consider the in vitro condition of the root culture and seedling phase of the ‘Corvina’ cultivar. Including all biological replicates, this project entailed the hybridization of about 168 samples. We used NimbleGen 12x135K arrays, which contain 12 x 135,000 probe sets and enable us to hybridize up to 12 independent samples on a single slide. The aim of the project is to observe the expression levels of transcriptional factor genes and signal transduction components, comparing them with those of metabolic genes. Moreover, it will explain if specialized expression patterns could be caused by preferential use of entire gene families in specific developmental processes or tissue-specific responses to the environment. 45 
O-26 Regulation of bud fruitfulness by floral meristem identity genes: unique version of the Arabidopsis model? O. Crane, A. Perl, E. Or* Department of Fruit Tree Sciences, Institute of Plant Sciences, Agricultural Research Organization, the Volcani Center, Bet Dagan, Israel *Corresponding author: vhettior@agri.gov.il Grapevine bud fruitfulness is determined by the differentiation of uncommitted meristems into either tendril or inflorescence. To study this issue, we first profiled expression in fruitful and non-fruitful buds along the same canes of a cane-pruned variety. We then carried out postgenomic analysis of master regulators of floral meristem identity (FMI) genes during development of these fruitful and non-fruitful buds. The interpretation of the results led us to analyze the effect of cytokinins on expression of these genes in tendrils, and compare their profiles in developing tendrils and during inflorescence development in bursting buds. Based on the integration of our findings, a new model will be proposed that suggests a high degree of conservation with the Arabidopsis model for regulation of floral meristem differentiation. 46 
Page 1 and 2: Date Time Session Topic Sunday Augu
Page 3 and 4: Monday August 2, 2010 Opening Plena
Page 5 and 6: Topic: Adaptation to soils and clim
Page 7 and 8: Tuesday August 3, 2010 Oral Session
Page 9 and 10: Poster Session 2 1:30 PM-3:00 PM Po
Page 11 and 12: P-105 Pathogen responsiveness and v
Page 13 and 14: Table of Contents Keynote Presentat
Page 15 and 16: K-2 Progress in grapevine breeding
Page 17 and 18: K-4 Genetics and Genomics of Grapev
Page 19 and 20: O-2 Additional homologs of the Ren1
Page 21 and 22: O-4 Y. Xu and Y.Wang* Introduction
Page 23 and 24: O-5 Identification of an R2R3 MYB t
Page 25 and 26: O-7 Sequencing of the phylloxera re
Page 27 and 28: O-9 Dissection of defense pathways
Page 29 and 30: O-11 Recent trends and technologies
Page 31 and 32: O-13 Brazilian Grape Breeding Progr
Page 33 and 34: O-15 Development of rootstocks for
Page 35 and 36: O-17 L. Wang* 1 , S. Li 1, 2 , P. F
Page 37 and 38: O-19 Integrated analysis of transcr
Page 39 and 40: O-20 Deciphering the chromosome str
Page 41 and 42: O-22 Identification of table grapes
Page 43: O-24 Metagenomic sequencing as a to
Page 47 and 48: O-28 Haplotype structure and cluste
Page 49 and 50: O-30 Annette Nassuth Function of Vi
Page 51 and 52: 10 to 25% of the chlorotic symptoms
Page 53 and 54: O-34 Cloning and characterization o
Page 55 and 56: O-36 New insight into the genetics
Page 57 and 58: O-38 Co-Localization of QTLs for Se
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96:
P-20 Characterization of key compon
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P-22 Expression of early light-indu
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P-24 The development of molecular m
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P-26 Identification of ABA inducibl
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P-28 In vitro selection of HYP tole
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P-30 In silico SNP detection for ge
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P-32 Vitis vinifera genome annotati
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P-34 Genome-wide identification of
Page 111 and 112:
P-36 Looking for genetic polymorphi
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P-38 Characterization of a new horm
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P-40 Towards a characterization of
Page 117 and 118:
the overall identified polymorphism
Page 119 and 120:
P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
Page 125 and 126:
P-48 ‘Cioutat’: a somatic varia
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P-50 Estimation of protein spot var
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glucosyltransferase, anthocyanidin
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P-53 Marker-free transgenic grapevi
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P-55 Agrobacterium rhizogenes-media
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P-57 Phenotypic evaluation of ‘Th
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P-59 Phenotypic divergence among gr
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P-61 Grepevine variety determinatio
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agricultural context. This network
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P-64 Genetic structure in a large r
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P-66 Analysis of grape rootstocks b
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P-68 Italian wild grapevine: a stat
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Rebula-Robola group were genotyped
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P-71 Viticultural performance of so
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P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
Page 159 and 160:
P-79 Studies on an excellent, early
Page 161 and 162:
P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
Page 187 and 188:
P-107 Screening grape hybrid famili
Page 189 and 190:
P-109 Characterization of an ankyri
Page 191 and 192:
P-112 Evaluation of grapevine germp
Page 193 and 194:
P-114 Evaluation of four new rootst
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