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P-37 A. Dereeper and P. This* SNiPlay, a web application for SNP analysis DIAPC INRA-Montpellier Supagro, Montpellier, France *Corresponding author: this@supagro.inra.fr Sequencing and large scale genetic diversity projects have simultaneously increased in grapevine. As a consequence, the need to automatically detect polymorphisms and exploit subsequent sequence and genotyping data are growing accordingly. Here, we present SNiPlay, a web-based tool dedicated to SNP discovery and polymorphism analysis. It integrates a pipeline, freely accessible through the internet, combining existing software to compute different kinds of analysis. From allelic data, alignment, or sequencing traces given as input (notably using the Polymorfind program), it detects SNP and insertion/deletion events. Then it sends sequences and allelic data into an integrative pipeline able to achieve successive steps: - Mapping on the Vitis vinifera (‘Pinot’) reference genome and SNP annotation (genomic position, intron/exon, synonyme/non synonyme) - Haplotype reconstruction (using softwares Phase, Gevalt or ShapeIT) - Haplotype network (Haplophyle) - Linkage Disequilibrium (Haploview) - Diversity analysis (SeqLib library) It also includes a database to store polymorphism and genotyping data produced by projects on grapevine, notably SNPGrapeMap and DLVitis projects. Private access allows collaborators to explore data: SNP retrieval using various filters (genomic position, missing data, polymorphism type), comparison of SNPs between populations or other external information about accessions (geographical origin and phenotypic data), and export of genotype data in different formats. This database can collect and combine genotyping data coming from sequencing project as well as those obtained by Illumina chips and provides a section facilitating comparison between experiments. Finally, we plan to add a specific module able to manage and detect SNP from Next-Generation Sequencing data soon. Furthermore, SNiPlay is flexible enough to easily incorporate in the future new modules able to manage other kinds of analyses such as kinship, association studies, or population structure. SNiPlay is available at http://sniplay.cirad.fr. 113 
P-38 Characterization of a new hormone in grape and analysis of its influence on grape development processes A. Keren-Keiserman*, A. Lichter, A. Perl, A. Ogredovitch, S. Lavee, E. Or Institute of Plant Sciences, The Volcani Center, Bet Dagan, Israel *Corresponding author: ettilab@agri.gov.il Phytosulfokines (PSKs) are secreted, sulfated peptide hormones derived from larger prepropeptide precursors. They are required for the proliferation and differentiation of plant cells in tissue culture and also promote cellular longevity. Recently it was realized that PSKs are involved in the attenuation of stress response and healing of wound-activated cells. Redundancy was confirmed between both PSK's receptors and several PSKα genes and phenotypes were observed only after parallel mutagenesis of several paralogs. Proteolytic processing of preproPSK precursors is dependent on a subtilisin-like serine protease. Four paralogs, of PSK and one PSK receptor (VvPSKR) have been isolated from Vitis vinifera and the genes were characterized. The expression profiles of VvPSK1 and VvPSK5 were analyzed in different organs, at different developmental and physiological stages, and in dormant buds subjected to oxidative and cold stresses. Transcripts are detectable in all tissues in basal levels and are induced during flowering and at fruit set, and later at véraison. Both genes are also highly expressed during embryo development and in dormant buds treated with dormancy release agents. VvPSK1 and VvPSKR were over expressed in embryonic cells and regenerated plants of V. vinifera and also in Nicotiana tabacum plants. The transgenic plants did not exhibit observable phenotypes. However, in contrast to cells grown in the dark, transgenic callus grown under long-day conditions exhibited yellow coloration. To further study the function of PSK in grape, we have developed an inducible RNAi construct to knock-down all four paralogs of the gene. This gene knock-down experiment is still underway. Our results support the model of stress-activated transcription, resembling the findings in Oryza sativa (rice) and Arabidopsis, where both transcriptional and post-translational modification of PSK documented. 114 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111: P-36 Looking for genetic polymorphi
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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