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P-96 Global gene response to viral infections in grapevine A. Vega, C. Medina*, R. Gutiérrez, P. Arce-Johnson P. Universidad Catholica de Chile, Fac. de Ciencias Biologicas, Santiago, Chile *Corresponding author: cmedina@bio.puc.cl Viral infections in grapevine develop compatible interactions, in which pathogens spread through all plant tissues without inducing a resistance response but affecting morphology of leaves, vegetative growth and productivity. Also, organoleptic properties, acidity and sugar content of the fruit are affected by the viral infections which affect the quality of the must produced from these infected plants. To investigate the global effect of virus infections in grapevine plants, transcriptional profiles of the red wine cultivar ‘Cabernet Sauvignon,’ which was naturally infected with GLRaV-3, were compared with virus-free grapevine plants. The Affymetrix GeneChip® Vitis genome array, version 1.0 was using for the analysis of leaves and berries at two maturation stages: veraison (EL-35) and maturation (EL-38). The infection was confirmed by RT-PCR and also by electronic microscopy that allowed us to observe virus particles in leaves and berries of the infected plants. A wide spectrum of biological functions was affected by viral infections in all tissues studied. The most relevant changes in gene expression in leaves were associated to synthesis and destination of proteins, transport and metabolism. Also developmental processes, senescence and cell defence were affected. In the fruit, the virus infection affects the maturation process, since a lower number of genes change expression at veraison and maturation stages in the infected plants compared with healthy plants. Besides, an important number of genes that appear down regulated in the infected fruits at maturation stage are associated with processes of biosynthesis of primary and secondary metabolites, metabolism, development process, senescence and cell defence. Therefore the maturation process appears incomplete in the infected plants. 177 
P-97 Genetic analysis of the resistance to foliar diseases on Muscadinia rotundifolia S. Blanc*, S. Wiedemann-Merdinoglu, P. Coste, V. Dumas, P. Mestre, D. Merdinoglu INRA-UDS, UMR1131 Santé de la Vigne et Qualité du Vin, Colmar, France *Corresponding author: sophie.blanc@colmar.inra.fr Viticulture is an important component of French agriculture, both in economical and cultural terms. Neverthless, a wide range of pathogens threatens French viticulture. The current strategy to control most grapevine diseases totally relies on the use of fungicides. This practice is not only expensive but also dangerous for the environment and human health. A cost-effective and environment friendly alternative to chemicals is the use of resistant varieties. However, all cultivated European grapevine varieties are susceptible to most of the pathogens, thus the resistance needs to be introduced from other Vitaceae through breeding programs. Muscadinia rotundifolia (2n=40) is a species closely related to the true Vitis (2n=38) and shows a high level of resistance to a wide spectrum of grapevine diseases. In order to analyse and exploit the syntheny between V. vinifera and M. rotundifolia and to accelerate the functional characterization of resistance to pathogens, we are developing a genetic map of M. rotundifolia, with a set of SSR markers scored on a 200 individuals S1 population of V. rotundifolia cv Regale. 150 markers out of 350 SSR have been found heterozygous, thus informative for mapping. Construction of the map is in progress. In parallel, the same population was tested for its resistance to downy mildew (Plasmopara viticola). Macroscopic and cytological observations on leaf discs allowed us to assess the level of resistance of these genotypes to downy mildew. The whole progeny is very resistant to P. viticola. Stomatic necrosis were frequently observed that may lead, in most cases, to stop the invasion of the pathogen when the biotrophic step of its life cycle starts, and then to inhibit sporulation. In few genotypes, downy mildew penetrate the leaf and develop haustoria and mycelium. In this case, a weak sporulation occurs very rarely. Our next goal will to align M. rotundifolia and V. vinifera genetic maps, in order to understand their degree of syntheny and to locate the position of the clusters of resistance genes in V. rotundifolia. The further analysis will help to understand the resistance mecanism of V. rotundifolia to pathogens. 178 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
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P-8 Monoterpene levels in different
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P-10 Usefulness of the SCC8 scar ma
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P-12 The onset of berry ripening is
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P-14 Morphological characteristics
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P-16 A genetic analysis of berry qu
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P-18 Use of molecular markers for s
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P-20 Characterization of key compon
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P-22 Expression of early light-indu
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P-24 The development of molecular m
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P-26 Identification of ABA inducibl
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P-28 In vitro selection of HYP tole
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P-30 In silico SNP detection for ge
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P-32 Vitis vinifera genome annotati
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P-34 Genome-wide identification of
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P-36 Looking for genetic polymorphi
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P-38 Characterization of a new horm
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P-40 Towards a characterization of
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the overall identified polymorphism
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P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175: P-95 Reaction of grape rootstocks t
Page 179 and 180: P-99 Berry cracking caused powdery
Page 181 and 182: P-101 Elicitation of grapevine defe
Page 183 and 184: P-103 The biological characteristic
Page 185 and 186: P-105 Pathogen responsiveness and v
Page 187 and 188: P-107 Screening grape hybrid famili
Page 189 and 190: P-109 Characterization of an ankyri
Page 191 and 192: P-112 Evaluation of grapevine germp
Page 193 and 194: P-114 Evaluation of four new rootst
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