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P-46 Defense response to downy mildew in grapevine: Learning from transcriptomics and metabolomics A. Figueiredo 1 , K. Ali 2 , A.M. Fortes 1 , F. Maltese 2 , Y.H. Young 2 , F. Monteiro 1 , S. Lisete 3 , M. Rex 4 , E. Zyprian 4 , R. Verpoorte 2 , M.S. Pais 1* 1 Unit of Molecular Biology and Plant Biotechnology, Centre for Biodiversity, Functional and Integrative Genomics, Science Faculty of Lisbon, Lisbon, Portugal; 2 Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University, Leiden, The Netherlands; 3 Department of Statistics and Operational Investigation, Center of Applied Statistics, Science Faculty of Lisbon, Lisbon, Portugal; 4 Julius Kühn Institute, Federal Research Centre for Cultivated Plants, Institute for Grapevine Breeding, Geilweilerhof, Siebeldingen, Germany *Corresponding author: msalomepais@gmail.com Grapevine (Vitis vinifera L.) is the most widely cultivated and economically important fruit crop in the world. With the global warming, fungal infections are becoming higher risk factors for wine producers. Plasmopara viticola [(Berk. and Curt) Berl. et De Toni], the causal agent of grapevine downy mildew, is one of the most devastating pathogens in viticulture. Fungicide application during the vegetative period has become obligatory since Vitis vinifera varieties are generally susceptible to this disease. To better understand the mechanisms underlying disease tolerance/resistance, cDNA microarrays and H 1 NMR-based metabolomics analyses were performed. Transcriptional and metabolic changes during the defence response of V. vinifera `Regent´ (a resistant grapevine cultivar) were accessed and compared to susceptible cultivar ‘Trincadeira’. Both cultivars were inoculated with P. viticola and analyzed at the following time points: 0h, 6hpi, 12hpi, 18hpi, 24hpi and 48hpi. So far, 22 transcripts were found to be differentially expressed at 0h. At 6hpi, 96 transcripts were found to be differentially expressed (54 up-regulated and 42 down-regulated). The main changes regarding the defense response at 6hpi are observed in the resistant cultivar. The transcript regulation suggests that the resistant cultivar perceives and reacts to the pathogen before the susceptible one. The multivariate data analyses, like Principal Component Analysis (PCA) and Partial Least Square-Discriminant Analysis (PLS-DA), of the 1 H NMR spectra showed evident innate differences between the resistant and susceptible cultivars in terms of primary and secondary metabolites possibly associated with the acquired resistance of grapevine against the pathogen. This study may contribute to the defining molecular and chemical markers of resistance to downy mildew in 123 
grapevine which will eventually be of high value for the rapid and precise selection of putative resistant genotypes in breeding programs. 124 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121: P-45 Preliminary observations on th
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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