conference schedule and program with abstracts - Horticulture ...

K-3
Grapevine genome update and beyond
A-F. Adam-Blondon* 1 and the French-Italian Public Consortium for the Sequencing of the
Grapevine Nuclear Genome 2
1 UMR INRA-UEVE URL CNRS Plant Genomics, Evry, France;
2 http://www.genoscope.cns.fr/externe/GenomeBrowser/Vitis/
*Corresponding author: adam@evry.inra.fr
The grapevine (Vitis vinifera L.) is the most economically valuable fruit crop in the world. Its
haploid genome is estimated to be about 500 Mb, organized in 19 chromosomes. The grapevine
is the fourth flowering plant whose genome sequence has been made public. A French-Italian
public consortium project has recently completed a 12X Whole Genome Shotgun sequence of a
quasi-homozygous genotype, PN40024. All data were generated by paired-end sequencing
plasmid, fosmid and BAC libraries of different insert sizes, using Sanger technology. Using
11.91X coverage, an assembly of 499 Mb was obtained, composed of 2888 super-contigs, 91%
of which are anchored on linkage groups. Different approaches revealed that approximately 41%
of the grape genome is of repetitive/transposable elements (TE) origin. The automatic annotation
led to an estimate of 26,347 protein coding gene models. The grape genome was shaped by two
ancient whole genome duplications that were not followed by extensive rearrangements, thus
enabling the discovery of ancestral traits and features of the genetic organization of flowering
plants. This sequence enables powerful integrative approaches for the identification of key genes
for important traits in grapevine and offers the potential to completely renew strategies for its
breeding.
17