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P-35 Evidence of a Ubiquitin Fusion Degradation 1 gene family in grapevine (Vitis vinifera) L. Wei*, J. Wu, J. Cheng Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China *Corresponding author: weilingzhu0409@126.com The ubiquitin fusion degradation 1 protein (UFD1) is an important ubiquitin recognition component in the ubiquitin-mediated degradation pathway of animals and yeast, but information about its occurrence and role in plants is scarce. Three putative UFD1 genes from grape were identified in this study. The data were confirmed by searching the publishing database from two independent grape genome projects with the obtained sequences. Three putative UFD1 genes containing the evolutionarily conserved UFD1 domains at the N-terminus were found on chromosome 3, chromosome 6 and chromosome 12, suggesting the presence of UFD1 genes in grapevine. These genes were compared with those in Arabidopsis, poplar, rice, wheat and maize through genome and EST databases mining. Unlike monocot species, having two UFD1 genes, dicot species have three UFD1 genes. Phylogeneically, the UFD1-like proteins from these six higher angiosperms fall into two distinct orthologous groups, which each includes a monocot clade and a dicot clade. One of the UFD1 paralogs in grape, Arabidopsis, and poplar has been duplicated through a segmental duplication event. 111 
P-36 Looking for genetic polymorphism through clonal variation of Pinot noir G. Carrier 1* , L. Le Cunff 1 , A. Dereeper 2 , S. Santoni 2 , L. Audeguin 1 , P. This 2 , J-M. Boursiquot 3 1 UMT GenoVigne, IFV-INRA-Montpellier Supagro, Montpellier, France; 2 UMR DIAPC INRA-Montpellier Supagro, Montpellier, France; 3 UMR Sciences pour l’oenologie INRA- Montpellier Supagro, Montpellier, France *Corresponding author: gregory.carrier@supagro.inra.fr Clonal variation among cultivars of Vitis vinifera L. has been known formany years and its use achieved very significant gains for the wine industry. Nowadays, the basis of clonal phenotypic variation is barely known: hypothesis of genetic mutations or epigenetic modifications have nevertheless been considered. The objective of this study is to get a better insight of the genetic basis of clonal variation to develop a tool for clonal identification and selection. The current scientific context, access at the whole genome sequences of the individual PN40024 derived from Pinot noir and of a heterozygous clone of Pinot noir ENTAV-INRA® 115 and the development of the NGS (new generation sequencing) technologies, allows now exhaustive studies of the grapevine genome. Towards this aim, we used the 454 GX FLX Titanium technology on three official clones of Pinot noir ENTAV-INRA® 386, 583 and 777, selected for their different phenotypic traits. We firstly developed a nuclear DNA extraction method to limit the contamination from cytoplasmic DNA. We then realized one 454 run by clone and we obtained in mean 1 million reads of 350 bases by run. Our data sets from the 454 and the contigs from the whole sequence of Pinot noir ENTAV-INRA® 115 have been aligned on the reference genome, PN40024 with a compilation of different software: Mosaik-assembler, and RepeatMasker. Sixty-six percent of the reads obtained from each of the 454 runs have been aligned at a unique locus. Thanks to NGS technologies, we could compare a large portion of the genome with 3X coverage: i) in mean 8Mb between Pinot noir ENTAV-INRA® 115 and with each of the three others clones and ii) 0.5 Mb between the three clones themselves. The different sequence data obtained from these clones will be compared to identify the major molecular events which separate the clones analyzed. Preliminary results showed that SNPs are less important than insertions/deletions to be polymorphic between clones. We will validate this polymorphism and will develop method to perform identification of the clones. 112 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109: P-34 Genome-wide identification of
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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