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P-44 Transcriptomic characteristics of an Oriental cultivar of Vitis vinifera ‘Koshu’ N. Goto-Yamamoto*, D. Kamioka, M. Numata National Research Institute of Brewing, Higashi-Hiroshima, Japan *Corresponding author: gotoh_n@nrib.go.jp Our previous studies have shown that ‘Koshu’, a native grape cultivar and an important whitewine grape in Japan, belongs to the Oriental cultivars of Vitis vinifera. ‘Koshu’ is well adapted to the humid summer in Japan, and shows higher disease resistance than Occidental cultivars. A hybridization experiment using biotin-labeled genomic DNA fragments to a Vitis vinifera GeneChip showed that the hybridization intensities of genomic DNA of ‘Koshu’ and those of Occidental cultivars (‘Cabernet Sauvignon’, ‘Pinot noir’, and ‘Chardonnay’) showed high correlation with a small number (about 40 out of 16,000 probe sets) of exceptions. This result showed that Vitis vinifera GeneChip is also useful for transcriptomic analysis of ‘Koshu’, even though major probe sets were designed from the EST data of Occidental cultivars, mainly ‘Cabernet Sauvignon’ and ‘Chardonnay’. Transcriptomic analysis of berry skins of ‘Koshu’ during maturation (three weeks after veraison) showed that the top 30 and 200 highly hybridized probe sets contained 12 and 19 grape ripening induced proteins (GRIPs), respectively, out of 27 GRIPs on the GeneChip, which is a similar tendency to that of ‘Chardonnay’. However, the comparison of the hybridization intensities of the berry skins of ‘Koshu’ and ‘Chardonnay’ from before the veraison stage to the harvest stage showed that the difference between ‘Koshu’ and ‘Chardonnay’ at the same stage is larger than that between the before-veraison stage and the harvest stage of the same cultivar. The berry skin of ‘Koshu’ highly expressed disease-resistance genes, e.g., Fiber protein Fb 19, PR-4 type protein, Class IV Chitinase, in addition to flavonoid pathway genes (‘Koshu’ accumulates a small amount of anthocyanin in the berry skin) and unclassified proteins, compared with ‘Chardonnay’. Thus, these transcriptomic characteristics of ‘Koshu’ are possibly one reason for the disease resistance and other characteristics of ‘Koshu’. 121 
P-45 Preliminary observations on the role of sirtuin genes in grapevine physiology M. Cucurachi 1 , B. Hubbard 2 , L.G. Kovacs 3 , M. Busconi 1 , C. Fogher 1 , R. Oláh 4 , P. Winterhagen 5 , A. Perl 6 , D.A. Sinclair 2 , L. Bavaresco* 7 1 Istituto di Agronomia, Genetica e Coltivazioni Erbacee, Sezione Botanica e Genetica Vegetale, Università Cattolica del Sacro Cuore, Piacenza, Italy; 2 Harvard Medical School, Pathology, Boston, USA; 3 Department of Fruit Science, Missouri State University, Mountain Grove, Missouri, USA; 4 Department of Genetics and Plant Breeding, Corvinus University of Budapest, Hungary; 5 Institute of Specialty Crops and Crop Physiology Fruit Sciences, University of Hohenheim, Stuttgart, Germany; 6 Institute of Plant Sciences, The Volcani Center, Bet Dagan, Israel; 7 Istituto di Frutti-Viticoltura, Università Cattolica del Sacro Cuore, Piacenza, Italy *Corresponding author: luigi.bavaresco@unicatt.it The sirtuin/Sir2 (Silent information regulator 2) family of NAD+-dependent deacetylases and mono-ADP-ribosyltransferases play a part in various aspect of cellular metabolism in eukaryotes. Their role in mediating the calorie restriction effect extending longevity and extending lifespan in Saccharomyces cerevisiae, Caenorhabditis elegans, and Drosophila melanogaster is well known. Information about their occurrence and role in plant genomes, however, is scarce. Recently, two putative sirtuin genes encoding a SIRT4-like protein on chromosome 7 and a SIRT7-like protein on chromosome 19, were identified in the V. vinifera genome. These two genes appear to be present in single copy as shown by Southern blotting analysis. RT-PCR results demonstrated a baseline transcription level of both genes in different plant organs. Sirtuin discovery in the grape genome is intriguing because stressed grapevine cells synthesize compounds with hydroxylated trans-stilbene ring structure, such as, resveratrol (trans 3,3′,5-trihydroxystilbene) which is able to activate sirtuins in yeast, in C. elegans and D. melanogaster. The aim of our research is the identification of the function and activity of both putative sirtuin proteins in grapevine. We will accomplish this by investigating their interaction with resveratrol using in vitro and in vivo approaches and assaying changes in their expression levels in response to various abiotic stress conditions. In vitro assays demonstrated a very weak NAD+-dependent deacetylase activity of both sirtuins and no activation by resveratrol. Moreover, neither protein displayed ADP-ribosyltransferase activity. The in vivo function will be investigated by transforming whole grapevine plants and cell cultures and by the omplementation of a S. cerevisiae sir2Δ mutant. 122 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119: P-43 Proteomic characterization of
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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