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P-27 Identification and Applications of Polyploid in Muscadine Grape (Vitis rotundifolia Michx.) Breeding X. Xu*, J. Lu, Z. Ren, F. Bradley Center for Viticulture and Small Fruit Research, College of Engineering Sciences, Technology, and Agriculture, Florida Agriculture and Mechanical University, 6505 Mahan Drive, Tallahassee, FL 32317 *Corresponding author: xia.xu@famu.edu Grape (Vitis L.) is a multibillion industry in the United States. Florida grape industry is an undeveloped agricultural enterprise because of serious disease problems. The muscadine grape (V. rotundifolia) is a native species and is very important for the regional grape production. Unfortunately, all muscadine grape cultivars contain large seeds that fall short the consumers’ expectation for table consumption. Seedlessness is one of the most desirable characters in developing new muscadine grape varieties. Attempts to introduce seedlessness from V. vinifera grapes into muscadine grapes have been made by regional breeders for decades and success has been rare due to genetic distance between these two genera. An alternative approach is to develop triploid seedless grapes. The potential value of tetraploids in the production of triploid grape has been recognized for many years in V. vinifera, and some varieties (V. complex) have been bred in Asia, particularly Japan and South Korea. The objectives of this research are to characterize tetraploids/aneuploids in muscadine grapes and to utilize them in triploid seedless muscadine grape breeding. Sixteen polyploids, including tetraploids (4x) and aneuploids, have been identified in muscadine grape (Vitis rotundifolia Michx.). Twelve of them resulted from colchicine treatments while the rest of them were identified from thousands of seedlings derived from open pollination of ‘Darlene’, ‘Pam’ and ‘Supreme’. The polyploidy status was confirmed by flow cytometry and chromosome counting. The polyploids were also morphologically distinguished from diploid muscadine origins showing earlier bud breaking, weaker vegetative growth, shorter internodes, greener and thinker leaves, fewer succulent stems, and earlier fruit ripening. Further investigation revealed that the polyploids had lower pollen viability with more floating seeds. The polyploids are being used for hybridizing with diploids to develop triploid (3X) seedless muscadine grape cultivars. 103 
P-28 In vitro selection of HYP tolerant cell lines from “Chardonnay” embryogenic suspension culture R. He* 1 , Y. Zhang 1,2 , C. Wang 1 , J. Wu 1,3 , L. Zhu 1 , X. Xu 2 , M.A. Walker 4 , J. Lu 2 1 College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China; 2 Center for Viticulture and Small Fruit Research, Florida A&M University, Tallahassee, Florida, USA; 3 Department of Horticultural Sciences, University of Florida, Gainesville, Florida, USA; 4 Department of Viticulture and Enology, University of California – Davis, USA *Corresponding author: rongronghe.smile@gmail.com Breeding for cold and salt tolerance of woody plants such as grapevine by classical methods is time consuming. An alternative approach to improve these traits of grapevine is to induce and select somaclonal variation using in vitro culture strategy. Suspension cultures of “Chardonnay” Proembryogenic Mass (PEM) were radiated with 10, 40, 60, 100, 500 Gy of Co60 and then transferred to the selection medium (Murashige and Skoog medium plus 0.5 µM 2, 4- Dichlorophenoxyacetic acid and 10 mM hydroxyproline). The hydroxyproline resistant cell lines were obtained from each dose of Co60 treatment (10, 40, 60, 100 Gy) except the 500 Gy, but most of them became brown after subcultures for several times. However, a stable cell line derived from radiation of Co60 at 100Gy dose was obtained, which was subsequently confirmed by hydroxyproline resistant test and proline content. After 4-week exposure to different concentrations of hydroxyproline, the resistant cell line eventually grew vividly on the MS medium plus 0.5 µM 2, 4-D and 4.0 mM hydroxyproline, while the control line turned brown. The resistant PEM also showed a higher free proline content than the control one after treated with hydroxyproline. The “resistant” PEM line of “Chardonnay” are germinated, and the plantlets are being used for further evaluation for salt, drought and cold tolerance. 104 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
Page 51 and 52: 10 to 25% of the chlorotic symptoms
Page 53 and 54: O-34 Cloning and characterization o
Page 55 and 56: O-36 New insight into the genetics
Page 57 and 58: O-38 Co-Localization of QTLs for Se
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101: P-26 Identification of ABA inducibl
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
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P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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