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P-13 Ripening-related Ethylene Responsive Factor characterization by Agrobacterium-mediated stable gene transfer in grapevine A. Dal Ri*, L. Dalla Costa, V. Poletti, L. Martinelli, C. Moser IASMA Research and Innovation Center, Fondazione Edmund Mach, S. Michele a/Adige I- 38010, Italy *Corresponding author: ant1781.dalri@gmail.com The onset of ripening in grape is characterized by a dramatic transcriptional change, but knowledge of the regulatory circuits and the transcription factors involved in this important process is still lacking. Although the grape berry is considered a non climacteric fruit, there is evidence of ethylene playing a role in fruit ripening, and we have recently identified four ethylene responsive factor (ERF) genes which are transcriptionally modulated at the veràison phase. ERF1 and ERF062 genes showed a great increase of expression at this developmental stage and remained highly expressed during ripening, whereas ERF3b and ERF4 transcripts were very abundant in the green berries but showed a minimum of expression at veràison. These gene expression profiles suggest they are possibly involved in ripening regulation. In order to understand the function of the ERF1 gene in grape, its over-expression and silencing were carried out by gene transfer technology in Vitis vinifera 'Brachetto'. The complete ERF1 coding sequence was cloned in pK7WG2 vector for over-expression, whereas a 209 bp ERF1 specific sequence was cloned in pK7GWIWG2 (II) for gene silencing. ‘Brachetto’ embryogenic callus was co-cultured for two days with Agrobacterium tumefaciens strain EHA105 carrying the binary vectors and then transferred to media with Timentin for Agrobacterium elimination and subsequently with kanamycin for the selection. Putatively transgenic embryos have been regenerated and induced to convert into plantlets, and molecular assays will be necessary to evaluate transgene insertion, copy number quantification, and expression. 89 
P-14 Morphological characteristics of cracking susceptible Korean table grapes, ‘Heukgoosul’ and ‘Tamnara’ I.-C. Son, S.-I. Oh, D. Kim* Department of Horticultural Science, Chungbuk National University, Cheongju, Korea *Corresponding author: dkpomo@chungbuk.ac.kr Though ‘Heukgoosul’ (‘Golden Muscat’ x ‘Pione’) and ‘Tamnara’ (‘Campbell Early’ x ‘Himrod Seedless’) are very promising new table cultivars bred in Korea, they have serious problems with berry cracking. The growth characteristics and morphology of berries was investigated to determine the factors affecting berry cracking among ‘Heukgoosul’, ‘Tamnara’, and cracking resistant ‘Hongisul’ and the two major cultivars, ‘Campbell-Early’ and ‘Kyoho’. Cluster weight was 457.3 g in ‘Heukgoosul’, 331.2 g in ‘Tamnara’ 321.8 g in ‘Hongisul’, 341.0 g in ‘Campbell- Early’, and 506.1 g in ‘Kyoho’. Berry weight was 11.19, 7.57, 5.01, 5.61, and 11.30 g respectively. The firmness of the pericarp, which influences berry cracking, was highest at 1.62 kg·Ø5mm -1 in ‘Hongisul’ compared with 1.39, and 1.38 kg·Ø5mm -1 of cracking susceptible ‘Heukgoosul’ and ‘Tamnara’ grapes. The berry splitting rate under critical turgor pressure was over 70% in ‘Heukgoosul’, ‘Tamnara’, and ‘Kyoho’ in order of ‘Hongisul’ < ‘Campbell Early’ < ‘Kyoho’ < ‘Heukgoosul’ < ‘Tamnara’. Critical turgor pressure was highest of 13.2 atm in ‘Campbell Early’ and was lowest of 5.7 atm in ‘Tamnara’ grape. The length of the fruit stalk and cap stem, which influences the denseness of berries in a cluster, were shortest at 159.5 and 6.25 mm in cracking susceptible ‘Heukguseul’. The breakdown of the stylar scar observed using SEM was seen in ‘Heukgoosul’, ‘Tamnara’, and ‘Kyoho’. Those cracking susceptible three cultivars also had irregular arrangement with larger cells in the transverse sectional structure of pericarp. As a result of morphological observation, berry cracking in the ‘Heukgoosul’ grape was influenced by the higher berry density in a cluster with shorter length of fruit stalks and cap stems and in ‘Tamnara’ was associated with structural weakness of pericarp with lowest berry firmness and critical turgor pressure. 90 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
Page 37 and 38: O-19 Integrated analysis of transcr
Page 39 and 40: O-20 Deciphering the chromosome str
Page 41 and 42: O-22 Identification of table grapes
Page 43 and 44: O-24 Metagenomic sequencing as a to
Page 45 and 46: O-26 Regulation of bud fruitfulness
Page 47 and 48: O-28 Haplotype structure and cluste
Page 49 and 50: O-30 Annette Nassuth Function of Vi
Page 51 and 52: 10 to 25% of the chlorotic symptoms
Page 53 and 54: O-34 Cloning and characterization o
Page 55 and 56: O-36 New insight into the genetics
Page 57 and 58: O-38 Co-Localization of QTLs for Se
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87: P-12 The onset of berry ripening is
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
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P-61 Grepevine variety determinatio
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agricultural context. This network
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P-64 Genetic structure in a large r
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P-66 Analysis of grape rootstocks b
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P-68 Italian wild grapevine: a stat
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Rebula-Robola group were genotyped
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P-71 Viticultural performance of so
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P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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