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P-80 Generation of a new polyploidy grape cultivar by a new strategy that combines crossbreeding with colchicine-induced mutation J. Chen, X. Tang, X. Ma, Q. Zhao, Z. Dong*, D. Li Shanxi Academy of Agricultural Sciences, China *Corresponding author: gssdzg1@163.com A new polyploid grape cultivar was generated by a new strategy that combines traditional crossbreeding with a chemical-induced mutation method. Germinated diploid hybrid grape seeds were treated with different concentrations of colchicine at 20⁰C during day and 25⁰C at night for a variety of time periods. Seedlings were examined morphologically for the initial mutation screening in the field. Comparison between seedlings from hybrid seeds and self-pollinated seeds indicated that the rate of deformity is 3.54% and 25.8% for hybrid and self-pollinated seeds, respectively. Analysis by tip-end slicing method to measure the size of the L 1 , L 2 , and L 3 of the meristem demonstrated a significant difference between diploid and tetraploid cultivars. Results from chromosome counting in root tips indicated that tetraploid descendants are 2n=4x=76 while the diploid parents are 2n=2x=38. Based on the size and shape of pollen and stomata and number of chloroplasts in guard cells, it was concluded that the new grape cultivars selected in this study have similar characteristics to ‘Black Olympia’ which is a well-known tetraploid cultivar. The data from pollen viability and fruiting rate also confirmed that cultivars we selected are tetraploids. By using this new strategy, we successfully generated several new polyploid cultivars that produce fruit earlier with high quality in terms of fruit size, seedlessness and taste. 161 
P-81 Effectiveness of different culture media on the success of embryo rescue among different crosses of seedless grapes Y. Hao* 1 , F. Wang 1 , R. Yang 1 , H. Wang 1 , J. Lu 2 1 Institute of Fruit and Floriculture Research ,Gansu Academy of Agricultural Sciences, Lanzhou, China; 2 College of Food Science and Nutrition Engineering, Agricultural University of China, Beijing, China *Corresponding author: haoyan7371@gmail.com Effects of different culture media were tested on embryo rescue among three different cross combinations of seedless grapes. Here ER, Nitsch, WP, half strength of B 5 and 1/2MS media were used. The best medium for ovule development was ER6-BA 0.5 mg/L among all the cross combinations. However, for rescued embryo regeneration and subculture, the most suitable media varied among different cross combinations. The best media for seedling establishment in ‘Flame seedless’ × ‘Centennia’ were 1/2 MS for embryo germination, 1/2 MS + IBA 0.1 mg/L for seedling growth, 1/2 MS + IBA 0.1 mg/L for subculture, respectively. The best media for ‘Otilia’ × ‘Venus’ were 1/2 MS + IAA 0.1 mg/L for embryo germination, 1/2 MS + IAA 0.1 mg/L for seedling growthand half strength of B 5 + IAA 0.125 mg/L for subculture, respectively. The best media for ‘Venus’ × ‘Himrod’ were 1/2 MS for embryo germination, WP + 6-BA 0.1 mg/L for seedling growth, half strength of B 5 + IAA 0.125 mg/L for subculture, respectively. 162 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
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P-8 Monoterpene levels in different
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P-10 Usefulness of the SCC8 scar ma
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P-12 The onset of berry ripening is
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P-14 Morphological characteristics
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P-16 A genetic analysis of berry qu
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P-18 Use of molecular markers for s
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P-20 Characterization of key compon
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P-22 Expression of early light-indu
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P-24 The development of molecular m
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P-26 Identification of ABA inducibl
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P-28 In vitro selection of HYP tole
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P-30 In silico SNP detection for ge
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P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159: P-79 Studies on an excellent, early
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175 and 176: P-95 Reaction of grape rootstocks t
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179 and 180: P-99 Berry cracking caused powdery
Page 181 and 182: P-101 Elicitation of grapevine defe
Page 183 and 184: P-103 The biological characteristic
Page 185 and 186: P-105 Pathogen responsiveness and v
Page 187 and 188: P-107 Screening grape hybrid famili
Page 189 and 190: P-109 Characterization of an ankyri
Page 191 and 192: P-112 Evaluation of grapevine germp
Page 193 and 194: P-114 Evaluation of four new rootst
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