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P-113 Analysis of genetic structure of twelve Sicilian grapevine cultivars E. Branzanti, A. Scienza, O. Failla*, L. Brancadoro Department of Crop Science, University of Milano, Milano, Italy *Corresponding author: osvaldo.failla@unimi.it In recent years, Sicilian viticulture has shown a significant growth with a remarkable increase of production, both in quantity and in quality. Sicily is a wide wine region in Italy and its wines have already made themselves known on the international markets. Several autochthonous grapevine cultivars have been preserved in Sicily, but this available resource is mainly unexploited because of the lack of wide genetic characterizations. The aim of the present study is to investigate molecular characteristics of Sicilian cultivars; evaluate probable cases of synonymies and false attributions and preserve Sicilian germplasm. A wide screening on 12 major Sicilian cultivars was carried out (Carricante, Catarratto comune e lucido, Nero d’Avola, Nerello cappuccio, Nerello Mascalese, Perricone, Grillo, Grecanico, Inzolia, Corinto nero and Frappato). A total of 687 accessions were sampled by the use of SSR markers (until 21 loci). Allelic profiles were obtained and confronted with other data banks, allowing to exclude some false attributions. Molecular analysis clarified several synonymies and false attributions: in particular there are many cases of Sangiovese cultivated as Nerello mascalese and Corinto nero; furthermore this comparison highlights that the 70% of Nerello cappuccio accessions is actually Carignan. A total of 25 unique and different SSRs profiles were found. Several cultivars, such as Perricone, Carricante and Grecanico, showed a high level of genotypic variability with different allelic profiles. No differences were detected among the accessions of Catarratto comune and Catarratto lucido; these cultivars have the same allelic profile. Parentage analysis suggested origins of some cultivars: Nero d’Avola, Grecanico, Catarratto and Inzolia are autochthonous while Nerello cappucio’s profiles can be closely related to some Calabrian cultivars. These results highlight the genetic richness and variability that is still present in the Sicilian region and that can be probably explained by its geographical isolation; furthermore, they provide a solid base for future studies and developments. 193 
P-114 Evaluation of four new rootstock genotypes obtained by backcross P. Carnevali, L. Brancadoro, O. Failla* Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Milan, Italy Corresponding author: osvaldo.failla@unimi.it In viticulture, the introduction of rootstocks was tied to the appearance of phylloxera in Europe in the late 1800s; today their use is strongly related to grapes adaptation to different pedoclimatic environments. If the extreme variability that characterizes agricultural habitats is taken to account, some specific and complex needs come out, such as the adaptation of rootstockscultivar pairs to soil features and the possible use of rootstocks for modulating the grapevine’s agronomical responses to edaphic and meteorological conditions. Starting from these assumptions, the introduction of new rootstocks can be a valuable contribution to the optimization of vineyard vegetative and productive responses. With this aim, the present research was started to compare four new rootstocks, obtained by Di. Pro. Ve. (University of Milan) through a back cross breeding selection, to six commercial ones (41B, 420A, 110R, 140Ru, 1103P and SO4). Four experimental vineyards (respectively placed in the “Valpolicella”, “Chianti Classico”, “Castel del Monte” and “Contea di Sclafani” A.O.C. areas) were planted with these ten rootstocks grafted to ‘Cabernet Sauvignon’ and a local variety. In those vineyards, 50 plants of each parcel (combinations of roostock-cultivar) were placed in randomized blocks, at about 5000 plants/ha and trained as a spur pruned cordon. From 2007 to 2009 and on a representative number of plants for each parcel some observations were made during vintage and winter pruning: agronomical parameters (Ravaz index, bud fertility, production weight/plant, bunches average weight, wood weight) and productive qualitative ones (pH, titratable acidity, soluble solids of musts, polyphenols and anthocyanins total contents of berry skins) were collected. Where it was possible, grapes were made into wines using a standard microvinification protocol, and wines were chemically analyzed and sensory evaluations were carried out. Our results showed that the new genotypes cause improved qualitative performances; have different capabilities to modulate cultivars’ vigor; induce high accumulations of soluble sugars in musts and good quantities of production/plant; and finally, are highly resistant to water stress. In conclusion, these new rootstocks could be introduced into commercial settings and could allow to better modulation of viticultural production in different pedo-climatic environments. 194 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
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P-8 Monoterpene levels in different
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P-10 Usefulness of the SCC8 scar ma
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P-12 The onset of berry ripening is
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P-14 Morphological characteristics
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P-16 A genetic analysis of berry qu
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P-18 Use of molecular markers for s
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P-20 Characterization of key compon
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P-22 Expression of early light-indu
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P-24 The development of molecular m
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P-26 Identification of ABA inducibl
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P-28 In vitro selection of HYP tole
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P-30 In silico SNP detection for ge
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P-32 Vitis vinifera genome annotati
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P-34 Genome-wide identification of
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P-36 Looking for genetic polymorphi
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P-38 Characterization of a new horm
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P-40 Towards a characterization of
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the overall identified polymorphism
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P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
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P-48 ‘Cioutat’: a somatic varia
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P-50 Estimation of protein spot var
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glucosyltransferase, anthocyanidin
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P-53 Marker-free transgenic grapevi
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P-55 Agrobacterium rhizogenes-media
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P-57 Phenotypic evaluation of ‘Th
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P-59 Phenotypic divergence among gr
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P-61 Grepevine variety determinatio
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Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175 and 176: P-95 Reaction of grape rootstocks t
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179 and 180: P-99 Berry cracking caused powdery
Page 181 and 182: P-101 Elicitation of grapevine defe
Page 183 and 184: P-103 The biological characteristic
Page 185 and 186: P-105 Pathogen responsiveness and v
Page 187 and 188: P-107 Screening grape hybrid famili
Page 189 and 190: P-109 Characterization of an ankyri
Page 191: P-112 Evaluation of grapevine germp
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