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P-94 Mechanisms of powdery mildew resistance in the Vitaceae family A. Feechan*, S. Kabbara, I.B. Dry CSIRO Plant Industry, Waite Campus, Urrbrae, SA 5064, Australia *Corresponding author: angela.feechan@csiro.au The cultivated grapevine, Vitis vinifera, is a member of the Vitaceae family which comprises over 700 species in 14 genera. Vitis vinifera is highly susceptible to the powdery mildew pathogen Erysiphe necator. However, other species within the Vitaceae family have been reported to show resistance to this fungal pathogen but little is known about the mechanistic basis of resistance in these species. Plants secrete cell wall materials at the site of attempted fungal infection in order to block penetration. If this penetration resistance is overcome some plant species have evolved resistance genes, the products of which trigger programmed cell death (PCD) in the presence of the host fungus. Therefore, the frequency of successful E. necator penetration events, in addition to PCD responses, were investigated in a range of different species within the Vitaceae family. The results reveal that penetration resistance and PCDmediated responses or combinations of both are employed by species from the different Vitaceae genera to limit E. necator infection. In order to further characterise the cellular processes involved in the observed penetration resistance, specific inhibitors of the actin cytoskeleton and secretory/endocytic vesicle trafficking function were employed. These inhibitors were demonstrated to successfully break penetration resistance in V. vinifera against the non-host powdery mildew E. cichoracearum. However, the use of these inhibitors with the host powdery mildew E. necator unexpectedly revealed that while secretory and endocytic vesicle trafficking pathways play a crucial role in non-host penetration resistance, the host powdery mildew species may actually require these pathways to successfully penetrate the plant host. 175 
P-95 Reaction of grape rootstocks to Meloidogyne incognita and M. javanica M,F. Moura 1* , C.R. Dias-Arieira 2 , H.H. Puerari 2,3 , M.A. Tecchio 1 , T.P.L. Cunha 2 , F.M. Chiamolera 2 1 Instituto Agronômico – IAC. Centro de Frutas, Jundiaí, São Paulo, Brazil; 2 Universidade Estadual de Maringá, Campus Regional de Umuarama, Umuarama, Paraná, Brazil; 3 Bolsista PIBIC: CNPq/IAC *Corresponding author: mouram@iac.sp.gov.br This study evaluates, under controlled conditions, the reaction of ten grape rootstocks from a germplasm collection from the Programa de Melhoramento Genético de Videira at the Centro APTA de Frutas do Instituto Agronômico de Campinas, Brazil, to Meloidogyne incognita and M. javanica nematodes. Seedlings were inoculated with 4,000 eggs and juveniles from these nematodes. After 60 days, radicular systems were evaluated with respect to the amount of galls in order to determine, through calculation of the reproduction factor, the immunity, resistance and susceptibility of rootstocks. Rootstocks ‘101-14’, ‘Teleki-5C’, ‘IAC 313’, ‘IAC 766’, ‘Kober 5BB’ and ‘Ripária do Traviú’ behaved as immune to both species of nematodes – ‘R99’ presented resistance. ‘IAC 571-6’ and ‘IAC 572’ behaved as resistant to M. incognita and immune to M. javanica; ‘420-A’ was immune to M. incognita and resistant to M. javanica. 176 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
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P-8 Monoterpene levels in different
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P-10 Usefulness of the SCC8 scar ma
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P-12 The onset of berry ripening is
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P-14 Morphological characteristics
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P-16 A genetic analysis of berry qu
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P-18 Use of molecular markers for s
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P-20 Characterization of key compon
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P-22 Expression of early light-indu
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P-24 The development of molecular m
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P-26 Identification of ABA inducibl
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P-28 In vitro selection of HYP tole
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P-30 In silico SNP detection for ge
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P-32 Vitis vinifera genome annotati
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P-34 Genome-wide identification of
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P-36 Looking for genetic polymorphi
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P-38 Characterization of a new horm
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P-40 Towards a characterization of
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the overall identified polymorphism
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P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173: P-93 Development of table and raisi
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179 and 180: P-99 Berry cracking caused powdery
Page 181 and 182: P-101 Elicitation of grapevine defe
Page 183 and 184: P-103 The biological characteristic
Page 185 and 186: P-105 Pathogen responsiveness and v
Page 187 and 188: P-107 Screening grape hybrid famili
Page 189 and 190: P-109 Characterization of an ankyri
Page 191 and 192: P-112 Evaluation of grapevine germp
Page 193 and 194: P-114 Evaluation of four new rootst
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