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P-106 Characterization of grapevine NPR1 Y. Zhang* and W. Qiu Center for Grapevine Biotechnology, William H. Darr School of Agriculture, Missouri State University, Mountain Grove, Missouri, USA *Corresponding author: zhangyiming522@gmail.com Non-expressor of Pathogenesis-Related genes 1 (NPR1) is a key transcription factor in the defense against pathogens by regulating defense-related genes in plants. Mutation of the NPR1-1 gene in Arabidopsis plants reduces transcript levels of pathogenesis-related (PR) genes including PR-1 and makes npr1-1 mutant more susceptible to pathogens. There are two NPR1 genes in the grapevine genome. It is known that these two grapevine NPR1 genes are activated by salicylic acid analog, but their functions are still based on the bioinformatics prediction. In the present study, one full-length NPR1 gene was cloned and sequenced from Vitis aestivalis ‘Norton’ and Vitis vinifera ‘Cabernet Sauvignon’, and referred to as VaNPR1.1 and VvNPR1.1, respectively, based on the high identity to the previously designated NPR1.1 of grapevine. Bioinformatics analysis indicated that predicted amino acid sequences of VaNPR1.1 and VvNPR1.1 differ by one amino acid. Over-expression of VaNPR1.1 gene in Arabidopsis npr1-1 mutant plants restores the expression of PR-1 gene, though not to the full scale. This result demonstrated that VaNPR1.1possesses similar function to Arabidopsis NPR1 in the regulation of defense-related genes and thus verified the function of grapevine NPR1.1 gene. 187 
P-107 Screening grape hybrid families with molecular markers linked to resistance genes D. Katula-Debreceni 1 , A. Veres 1 , A.K. Lencsés 1 , A. Szőke 1 , P. Kozma 2 , L. Kovács 3 , E. Kiss* 1 1 Szent István University, Institute of Genetics and Biotechnology, Páter K. u. 1. H-2100 Gödöllő, Hungary; 2 Research Institute of Viticulture and Enology, Pázmány P. u. 4. H-7634 Pécs, Hungary; 3 Missouri State University, Springfield, Missouri, USA *Corresponding author: kiss.erzsebet@mkk.szie.hu Gene pyramiding is a method to breed grape varieties for durable disease resistance. The use of resistance gene-linked DNA markers reduces the volume of breeding experiments, since it facilitates the identification of progeny seedlings that have inherited the desired gene shortly after germination and allows the reduction of population size. Saturating the grape genome with molecular markers, the construction of genetic linkage maps, and the recent publication of the Vitis vinifera genome sequence enable breeders to select the desired genotype directly. To combine powdery and downy mildew resistance genes, Kozma et al. (Research Institute of Viticulture and Enology, Pécs) produced the following hybrid families: BC 4 (VRH 3082-1-42) x ’Kishmish vatkana’, BC 4 x ’Kishmish moldavskij’, ’Génuai zamatos’ x ’Kishmish vatkana’, (’Laszta’ x. ’Dzhandzhal kara’) x (’Katta kurgán x Perlette’), where BC 4 derives from a V. vinifera x Muscadinia rotundifolia cross. Our aim was to select the individuals containing the powdery (PM) and/or downy mildew (DM) major resistance genes of different origin (Muscadinia rotundifolia-Run1, Rpv1, V. vinifera-Ren1, and PM and DM QTLs of ’Seyve- Villard’) with SSR, CB and SCAR markers. PCR products were separated on 8% polyacrilamide (ALFExpress) and 4% Metaphor gel. Our data corroborated earlier findings that the M. rotundifolia-derived Rpv1 and Run1 loci are closely linked. We compared the symptomless progenies derived from the cross BC 4 x ’Kishmish vatkana’ and (’Laszt’ x ’Dzsandzsal kara’) x (’Katta kurgán’ x ’Perlette’) using resistance linked markers. We observed that alleles of SSR markers linked to the PM resistance gene Ren1 in the linkage group 13 are the same, suggesting that PM resistance locus of ’Kishmish vatkana’ and ’Dzhandzhal kara’ are presumably identical. Studies of QTL markers are in progress. 188 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
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P-8 Monoterpene levels in different
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P-10 Usefulness of the SCC8 scar ma
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P-12 The onset of berry ripening is
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P-14 Morphological characteristics
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P-16 A genetic analysis of berry qu
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P-18 Use of molecular markers for s
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P-20 Characterization of key compon
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P-22 Expression of early light-indu
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P-24 The development of molecular m
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P-26 Identification of ABA inducibl
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P-28 In vitro selection of HYP tole
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P-30 In silico SNP detection for ge
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P-32 Vitis vinifera genome annotati
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P-34 Genome-wide identification of
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P-36 Looking for genetic polymorphi
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P-38 Characterization of a new horm
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P-40 Towards a characterization of
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the overall identified polymorphism
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P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
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P-48 ‘Cioutat’: a somatic varia
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P-50 Estimation of protein spot var
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glucosyltransferase, anthocyanidin
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P-53 Marker-free transgenic grapevi
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P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175 and 176: P-95 Reaction of grape rootstocks t
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179 and 180: P-99 Berry cracking caused powdery
Page 181 and 182: P-101 Elicitation of grapevine defe
Page 183 and 184: P-103 The biological characteristic
Page 185: P-105 Pathogen responsiveness and v
Page 189 and 190: P-109 Characterization of an ankyri
Page 191 and 192: P-112 Evaluation of grapevine germp
Page 193 and 194: P-114 Evaluation of four new rootst
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