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P-67 G. Jahnke*, J. Májer, B. Szőke Analysis of pinot varieties by microsatellite markers Research Institute for Viticulture and Oenology, Centre of Agricultural Sciences, University of Pannonia , Badacsony, Badacsonytomaj, Hungary *Corresponding author: gjahnke@mail.iif.hu Molecular markers are widely used because of the wide range of applications. The identification of the varieties and clones of Pinots by microsatellite method according to the literature, present difficulties Hocquigny et al (2004) carried out SSR analysis between Pinots. Polymorphism analysis between ‘Pinot’ clones revealed that 65% shared the most frequent genotype. Moreover, the variant clones showed at least 96% similarity with this genotype. Based on our former experiences 7 Pinot gris, 4 Pinot noir clones and Pinot blanc were analysed in 16 (VVS2, VMC5E9, VMC3D12, VVIM10, VMC5G8, VMCNG1E1, VMC1F10, VMC2H4, VMC8A7, VMC7G3, VVMD28, VrZag21, VrZag79, VMC1C10, VrZag25, Scu06vv) microsatellite loci. A dendogramm was constructed using Jaccard index for the estimates of genetic similarity between pairs, and average linkage for clustering. Based on our results, it can be established, that the Pinot clones all showed high similarity. The Pinot gris clones bred in Badacsony, Hungary (B. 10, B. 10/5, B. 10/10) formed a group and showed the highest similarity with Pinor gris 34 from Romania. The other Pinot gris clones formed another group with Pinot noir C-162. These clones all originated from western Europe (Germany, France). These genetic differences could be traced back to the different geographical origin of the different clones. 147 
P-68 Italian wild grapevine: a state of the art on germplasm and conservation in 2010, the Year of Biodiversity S. Imazio * , B. Biagini, G. De Lorenzis, A. Scienza, O. Failla Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Milano, Italy    *Corresponding author: serena.imazio@unimi.it Vitis vinifera sylvestris is the wild progenitor of cultivated grapevine. Wild grapevine populations are part of the European woodlands. In some countries wild grapevine is considered on the brink of extinction. In 2006 the Italian Ministry of Environment funded a research project aiming to the creation of a germplasm collection of wild grapevines pooled from Italian existing populations. The project involved the Milano University and the Cinque Terre National Park which is characterized by an agricultural landscape made up of terraces cultivated once almost exclusively by vines held up by stone walls. Today most of the terraces are still present but are at constant risk of landslides due to their abandonment. Cinque Terre was designated a UNESCO world heritage site for its landscape agriculture and for the need of preservation of its cultural, environmental, local and historical values. One of the purposes of the collection inserted in this frame is to recover a part of the abandoned terraces. Moreover the experimental vineyard has both the characteristics of in situ and ex situ collection and is dedicated to researchers interested in the study of the genetic structure of wild grapevine, both for population genetics and for studies regarding gene diversity (in the wild and cultivated compartment). For this reason 600 samples representative of Italian wild grapevine populations widely dispersed in woodlands have been collected. At this time 300 among these are already in the collection and have been genotyped at 20 SSR loci both, to have a correct fingerprint of all the accessions and to avoid mistakes during samplings. Based on molecular data and on the comparison with previous censuses some considerations about the genetic structure and conservation of Italian wild grapevine populations and their level of introgression with Italian cultivated germplasm have been made. In the next years the collection will be monitored to collect data regarding phenology and possible pest tolerance; and to improve knowledge on genetic traits differentially expressed in the two subspecies. In the year devoted to biodiversity, this collection has the intent to highlight two important features: one related to the safeguard of an harsh land imprinted and tamed by agricultural activities and human labour; the other devoted to the understanding and use of the genetic resources present in the wild grapevine compartment evolved in a natural environment untouched by human activities. 148 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
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P-8 Monoterpene levels in different
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P-10 Usefulness of the SCC8 scar ma
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P-12 The onset of berry ripening is
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P-14 Morphological characteristics
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P-16 A genetic analysis of berry qu
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P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145: P-66 Analysis of grape rootstocks b
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175 and 176: P-95 Reaction of grape rootstocks t
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179 and 180: P-99 Berry cracking caused powdery
Page 181 and 182: P-101 Elicitation of grapevine defe
Page 183 and 184: P-103 The biological characteristic
Page 185 and 186: P-105 Pathogen responsiveness and v
Page 187 and 188: P-107 Screening grape hybrid famili
Page 189 and 190: P-109 Characterization of an ankyri
Page 191 and 192: P-112 Evaluation of grapevine germp
Page 193 and 194: P-114 Evaluation of four new rootst
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