conference schedule and program with abstracts - Horticulture ...

O-20
Deciphering the chromosome structure in Vitis vinifera: genetic mapping, BAC-FISH and
recombination
A. Canaguier* 1 , I. Le Clainche 1 , A. Bérard 4 , A. Chauveau 4 , M-S. Vernerey 2 , C. Guichard 1 , P-Y.
Bourguignon 3,6 , M-C. Le Paslier 4 , O. Jaillon 3 , G. Di Gaspero 5 , O. Coriton 2 , D. Brunel 4 , A-F.
Adam-Blondon 1
1 UMR de Génomique Végétale, INRA-CNRS-UEVE, Evry, France; 2 UMR Amélioration des
Plantes et Biotechnologies Végétales, INRA Agrocampus Rennes, Université de Rennes, Le
Rheu, France; 3 Genoscope, IG-CEA, Evry, France; 4 INRA, UR EPGV, CEA / Institut de
Génomique, Centre National de Génotypage, Bat G2, Evry, France; 5 Istituto di Genomica
Applicata, Parco Scientifico e Tecnologico Luigi Danieli, Udine, Italy; 6 Max Planck Institute for
Mathematics in Science Inselstraße, Leipzig, Germany, and Laboratoire de Physique Statistique
CNRS - Univ. Paris VI and VII - Ecole Normale Supérieure 24, Paris, France
*Corresponding author: canaguie@evry.inra.fr
Within the framework of the sequencing project for the grapevine genome (Jaillon et al 2007),
two genetic maps were completed with SSR markers in order to align the sequence contigs along
the chromosomes. Thus, two full sib populations, ‘Syrah’ x ‘Grenache’ (SYxGR, 192
individuals; Doligez et al. 2006) and ‘Chardonnay’ x ‘Bianca’ (CHxBI, 358 individuals; Doligez
et al. 2006) on which, respectively, 223 and 401 markers have been used. These two maps
allowed anchoring over 90% of the reference genome sequence (Cipriani et al. in prep.).
Recently, SNP markers were defined from 38 anchored but not oriented super-contigs of
sequence and 44 super-contigs not oriented and not anchored. So far 110 new markers were
mapped, allowing anchoring of 21 not yet anchored super-contigs (~3.2Mb) and orienting 32
super-contigs. In depth analysis is ongoing. In parallel, the recombination rate has been analyzed
along the chromosomes for each of the four parents using the first version of the two maps with
the software TETRA (Drouaud et al 2006). This software associates a probability with the
observation of hot spots or cold spots of recombination. Thus, the recombination rates have been
compared with the density of repeats sequences, genes, and GC%. Moreover, we are developing
a cytogenetic map in Vitis vinifera using BAC-FISH. All these approaches will be combined to
give a pattern of the Vitis vinifera genome, including the factors affecting its recombination rates
and the dynamics of the genome evolution.
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