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P-76 Aestivales (Planchon), American Native Grapes: Phylogenetics and Use for Breeding of High Quality Wine Grapes for Southeastern United States T.A. Mongerie, J.L. Boutne, S. Krastanova, S. Leong, V.M. Colova (Tsolova)* Florida A&M University, CESTA, Center for Viticulture and Small Fruit Research, 6505 Mahan Drive, Tallahassee, FL 32317, USA *Corresponding author: violetka.colova@famu.edu Cultivars of ‘Aestivales’ are the only American native grapes with remarkable juice and wine color stability. The native grapes of the Americas have provided valuable germplasm for improvement and development of cultivated commercial grape genotypes for fresh fruit, wine and processing throughout the United States and Canada, where Vitis vinifera, or “old world grape”, can not grow. Also they have qualities for wine longevity and very pleasant “mouth feel”. Members of the ‘Aestivales’ group including Vitis aestivalis Michaux, are found in the eastern and central United States, from New England to Florida and from Wisconsin to Texas. The close proximity of related species and many variants to Vitis aestivalis has created confusion among taxonomists trying to classify grape species. The aim of this research study was to define the phylogenetic relations among the grape species and subspecies listed under the ‘Aestivales’ Planchon group via data mining in the existing North American grape germplasm collections, and specifically expressed in the members of the group DNA microsatellites for using them in breeding programs and grape improvement. DNA isolation and quantification of nine ‘Aestivales’ accessions, evenly distributed throughout their area of natural habitat was completed. Microsatellite specific PCR amplification products were obtained in nine out of ten microsatellite markers from V. riparia which were previously and successfully used for grape identification. The amplicons were subjected to fragment analysis and then analyzed using the software STATISTICA, version 4.5 and a dendrogram was generated. Results found using the Applied Biosystems Sequencer (ABI) showed two distinct groups, the first group had accessions acquired from the south and midwest while the second group had accessions acquired from the north. Thus indicates that there was a clear delineation between the origin of the accessions and proximity of their relationship in the dendrogram. As a spin out of the “pedigree” research of “Cynthiana” var. the comprehensive germplasm collection of Aestivales, Planshon native grapes was established and was used as genetic pool for further directed hybridizations. More than 100 of the new selections were planted and are currently under evaluation for desirable quality characteristics. 157 
P-77 Morphological and molecular identification of Maragheh grapevine cultivars Y. Sharafi* 1 and A. Bfmani 2 1 Islamic Azad University-Maragheh Branch, Maragheh, Iran; 2 Department of Horticulture, University of Maragheh, Maragheh, Iran *Corresponding author: yavarsharafi@gmail.com Maragheh, Iran, has a long history in viticulture, and grapes (Vitis vinifera, L) are one of the most important commercial fruit crops grown and used for raisins, juice and fresh fruit. Most of the cultivars are unknown to growers and breeders however, and their genetic characterization is necessary to the growing and breeding programs. The goal of this study was to identify the cultivars planted in Maragheh, based on morphological and molecular information, for planning the growing and breeding programs. Fifty cultivars and their morphological traits were characterized through ampelography and 14 SSR primers were used to confirm the results. Results showed significant differences among all of the studied cultivars based on morphological characteristics. The SSR primers chosen for this study discriminated 30 different genotypes among the 50 analyzed cultivars. 158 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
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P-8 Monoterpene levels in different
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P-10 Usefulness of the SCC8 scar ma
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P-12 The onset of berry ripening is
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P-14 Morphological characteristics
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P-16 A genetic analysis of berry qu
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P-18 Use of molecular markers for s
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P-20 Characterization of key compon
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P-22 Expression of early light-indu
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P-24 The development of molecular m
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P-26 Identification of ABA inducibl
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P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155: P-75 Variation of berry polyphenoli
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175 and 176: P-95 Reaction of grape rootstocks t
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179 and 180: P-99 Berry cracking caused powdery
Page 181 and 182: P-101 Elicitation of grapevine defe
Page 183 and 184: P-103 The biological characteristic
Page 185 and 186: P-105 Pathogen responsiveness and v
Page 187 and 188: P-107 Screening grape hybrid famili
Page 189 and 190: P-109 Characterization of an ankyri
Page 191 and 192: P-112 Evaluation of grapevine germp
Page 193 and 194: P-114 Evaluation of four new rootst
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