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O-4<br />

Y. Xu <strong>and</strong> Y.Wang*<br />

Introduction <strong>and</strong> application of Chinese wild Vitis species in China<br />

College of <strong>Horticulture</strong> <strong>and</strong> Shaanxi Key Laboratory of Molecular Biology for Agriculture,<br />

Northwest A&F University <strong>and</strong> Key Laboratory of Horticultural Plant Germplasm Resource<br />

Utilization in Northwest China, Ministry of Agriculture Yangling, Shaanxi, China<br />

*Corresponding author: wangyj@nwsuaf.edu.cn<br />

China is one of major centers of origin of the Vitis species. Chinese wild Vitis species are<br />

naturally distributed in the majority of areas throughout China, but mainly distributed in<br />

Huazhong Area <strong>and</strong> Qinba Mountain Area located in Shaanxi <strong>and</strong> Gansu provinces in China. To<br />

date, more than 35 species <strong>and</strong> varieties have been reported <strong>with</strong> origins in China among the<br />

approximately 70 known species. In the past 20 years, Researchers have focused on the study of<br />

the resistance to diseases Uncinula necator, Plasmopara viticola, Sphaceloma ampelinum,<br />

Coniathyrium diplodiella <strong>and</strong> Glomerella cingulata of Vitis species or varieties native to China<br />

by means of field identification, field inoculation <strong>and</strong> laboratory isolated inoculation. The results<br />

indicated that the species <strong>and</strong> clones indigenous to China are resistant or highly resistant to the<br />

diseases. There are differences in resistance to the diseases among all these species <strong>and</strong> clones.<br />

However, the Chinese wild V. pseudoreticulata accession “Baihe-35-1” hold higher resistance to<br />

disease traits than the other wild species. Cold hardiness of 17 wild Vitis species native to China,<br />

including 38 clones, was assessed by growth, tissue browning <strong>and</strong> electrolytic conductance<br />

methods. The results showed that the cold hardiness of V. amurensis <strong>and</strong> V. yeshanensis were<br />

highest as compared to other species. Chinese Vitis species have many advantages over V.<br />

vinifera such as possessing many resistant genes to main fungi diseases as well as good flavor.<br />

Especially V. quinquangularis <strong>and</strong> V. amurensis clones hold better economical traits than the<br />

other wild species <strong>and</strong> many disease resistant genes. So, they are used as wine materials directly<br />

in southwest <strong>and</strong> northeast areas in China; <strong>and</strong> they exhibited high resistance to fungal diseases,<br />

they were also easier to hybridize <strong>with</strong> V. vinifera. Thus, it is possible to use crossing to integrate<br />

fungal disease-resistant traits from the wild Chinese Vitis into V. vinifera, thus resulting in fungal<br />

disease-resistant new grape varieties. At present, we are interested in studying how Chinese wild<br />

grapes sense <strong>and</strong> respond to fungal diseases using V. pseudoreticulata accession Baihe-35-1 as<br />

material. Particularly, we are interested in underst<strong>and</strong>ing regulation of disease resistance<br />

signaling mechanisms <strong>and</strong> disease resistance genes under Uncinula necator stress in Chinese<br />

wild Vitis species. Present research involves analysis of function of target genes using biochemical<br />

method in vitro, <strong>and</strong> also genetic <strong>and</strong> molecular characterization of Arabidopsis<br />

mutants that are altered in response to the pathogen stimuli. The goals are to elucidate the<br />

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