conference schedule and program with abstracts - Horticulture ...
conference schedule and program with abstracts - Horticulture ...
conference schedule and program with abstracts - Horticulture ...
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P-81<br />
Effectiveness of different culture media on the success of embryo rescue among different<br />
crosses of seedless grapes<br />
Y. Hao* 1 , F. Wang 1 , R. Yang 1 , H. Wang 1 , J. Lu 2<br />
1 Institute of Fruit <strong>and</strong> Floriculture Research ,Gansu Academy of Agricultural Sciences, Lanzhou,<br />
China; 2 College of Food Science <strong>and</strong> Nutrition Engineering, Agricultural University of China,<br />
Beijing, China<br />
*Corresponding author: haoyan7371@gmail.com<br />
Effects of different culture media were tested on embryo rescue among three different cross<br />
combinations of seedless grapes. Here ER, Nitsch, WP, half strength of B 5 <strong>and</strong> 1/2MS media<br />
were used. The best medium for ovule development was ER6-BA 0.5 mg/L among all the<br />
cross combinations. However, for rescued embryo regeneration <strong>and</strong> subculture, the most suitable<br />
media varied among different cross combinations. The best media for seedling establishment in<br />
‘Flame seedless’ × ‘Centennia’ were 1/2 MS for embryo germination, 1/2 MS + IBA 0.1 mg/L<br />
for seedling growth, 1/2 MS + IBA 0.1 mg/L for subculture, respectively. The best media for<br />
‘Otilia’ × ‘Venus’ were 1/2 MS + IAA 0.1 mg/L for embryo germination, 1/2 MS + IAA 0.1<br />
mg/L for seedling growth<strong>and</strong> half strength of B 5 + IAA 0.125 mg/L for subculture,<br />
respectively. The best media for ‘Venus’ × ‘Himrod’ were 1/2 MS for embryo germination, WP<br />
+ 6-BA 0.1 mg/L for seedling growth, half strength of B 5 + IAA 0.125 mg/L for subculture,<br />
respectively.<br />
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