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conference schedule and program with abstracts - Horticulture ...
conference schedule and program with abstracts - Horticulture ...
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P-48<br />
‘Cioutat’: a somatic variant of ‘Chasselas’ exhibiting a <strong>program</strong>med<br />
cell death-like phenotype<br />
D. Lijavetzky 1 *, L. Fern<strong>and</strong>ez, J. Diaz-Riquelme, J-M. Martinez-Zapater<br />
1<br />
Instituto de Biologia Agricola de Mendoza (IBAM), CONICET/FCA-UNCuyo, Mendoza,<br />
Argentina<br />
*Corresponding author: diegolija@hotmail.com<br />
Somatic genetic variation constitutes an important source of phenotypic variation in grapevine.<br />
In perennial plant species where mutants are difficult to generate <strong>and</strong> screen, spontaneous<br />
somatic variants represent a unique resource to underst<strong>and</strong> the genetic control of complex<br />
developmental patterns. ‘Cioutat’ is somatic variant of cultivar ‘Chasselas’ displaying parsleylike<br />
leaves. Genetic analysis of progeny of a ‘Cioutat’ self-cross population showed 25% plants<br />
<strong>with</strong> wild-type (‘Chasselas’) phenotype, consistent <strong>with</strong> the hypothesis that the phenotype is<br />
caused by a single dominant mutation. Transcriptomic comparison of ‘Cioutat’ <strong>and</strong> ‘Chasselas’<br />
revealed significant over-expression of a BAG (Bcl-2 associated athanogene) gene closely<br />
related to the Arabidopsis AtBAG6 gene in the ‘Cioutat’ leaf primordia. We named that<br />
grapevine gene as VvBAG6. Interestingly, in Arabidopsis, AtBAG6 is suggested to be a stress<br />
upregulated protein involved in <strong>program</strong>med cell death (PCD). Moreover, a quantitative<br />
evaluation of the ‘Cioutat’ phenotype in the self-cross segregant population showed a positive<br />
<strong>and</strong> statistically significant correlation between VvBAG6 expression level <strong>and</strong> the extension of<br />
the parsley leaf phenotype evaluated in the same plants. Collectively, these results suggest the<br />
involvement of PCD in the generation of the ‘Cioutat’ phenotype.<br />
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