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P-79<br />

Studies on an excellent, early-maturing bud sport of ‘Kyoho’<br />

J. Wu 1 , L. Wei* 1 , L. Jin 2 , J. Cheng 1<br />

1 Institute of <strong>Horticulture</strong>, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang,<br />

China; 2 Lianyu Institute of Grape Science, Leqing, Zhejiang, China<br />

*Corresponding author: weilingzhu0409@126.com<br />

The ‘Kyoho’ grape is a European-American hybrid cultivar <strong>with</strong> strong disease resistance <strong>and</strong><br />

wide adaptability. Fortunately, we found an excellent bud mutation of ‘Kyoho’ from orchard by<br />

selection. The bud mutation matured seven days earlier than ‘Kyoho’ in phenology for five<br />

consecutive years. The berry weight averaged 12-15 g. The berry size <strong>and</strong> the content of soluble<br />

sugar, pulp firmness, bearing pull force were also improved compared to those of ‘Kyoho’.<br />

Using the bud mutation as scion, the appropriate rootstocks were determined from those widely<br />

used in the south of China. To evaluate the genetic relationship between the bud mutation <strong>and</strong> its<br />

maternal parent ‘Kyoho’, 42 grapevine simple sequence repeat (SSR) markers were used to<br />

amplify DNA of ‘Kyoho’ <strong>and</strong> its bud mutation. These microsatellites are highly polymorphic <strong>and</strong><br />

have been considered suitable for assessing variation among grapevine collections. The results<br />

showed the genetic similarity from these SSR markers is 97.6% <strong>and</strong> a pair of SSR primers<br />

VrZAG67 can be used to distinguish bud mutation from ‘Kyoho’.<br />

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