conference schedule and program with abstracts - Horticulture ...

O-54
The study of triploid progenies from crosses between diploid and tetraploid grapes
L. Sun*, A.L. Yan, G.J. Zhang, W. Wang, H.Y. Xu
Institute of Forestry and Pomology, Beijing Academy of Agricultural and Forestry Science,
Beijing, China
*Corresponding author: sunlei.bjfu@gmail.com
Crossing of plants with different ploidys is one of the effective ways to obtain new seedless
cultivars. In this paper, through testing the changes of the ovule weight and observing its
anatomical structure, the period and mode of ovule abortion in crosses between diploid and
tetraploid grape cultivars were studied and reciprocal crossing was also conducted between them.
The results indicated that the proper sampling period is one of the most important factors in
ovule culture, and unlike in seedless cultivars, the embryo abortion did not occur at the same
time for most seeded cultivars crossed with different ploidy plants, but the ovule abortion was
only individual behavior. The period of ovule abortion was correlated with the maturing period
of the female parent. The abortive embryo of early-maturing cultivars occurred at five weeks
after pollination, and the mid-maturing ones and late-maturing ones were at six and nine weeks
after pollination, respectively. The reciprocal crossing results showed that this crossing form has
great mating obstruction, and the situation was more serious when tetraploid cultivars were used
as the female parent. Very few seeds from 2X × 4X had good germinability, while those from 4X
× 2X lost their germinability and no seedlings were obtained. The highest germination rate and
seedling survival rate were obtained when the ovule was excised 60 days after pollination, and it
is easier to harvest hybrid progeny when using diploid as female parent.
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