conference schedule and program with abstracts - Horticulture ...
conference schedule and program with abstracts - Horticulture ...
conference schedule and program with abstracts - Horticulture ...
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P-105<br />
Pathogen responsiveness <strong>and</strong> variety/tissue specificity of five stilbene synthase genes in<br />
grapevine<br />
R. Dai*, M.B. Ali, W. Qiu<br />
Center for Grapevine Biotechnology, William H. Darr School of Agriculture, Missouri State<br />
University, Mountain Grove, Missouri, USA<br />
*Corresponding author: ru0201@live.missouristate.edu<br />
Grapevine stilbenic compounds have antimicrobial activities <strong>and</strong> some of them including<br />
resveratrol are beneficial to human health. Stilbene synthase (STS) is the key enzyme that<br />
catalyzes the biosynthesis of stilbenic compounds. Grapevine genome contains 43 STS genes,<br />
<strong>and</strong> the regulation of each STS gene needs to be understood for defining their roles. In this study,<br />
we selected five STS genes <strong>and</strong> employed quantitative polymerase chain reaction (qPCR) to<br />
characterize their transcriptional profiles during the berry development <strong>and</strong> infection by the<br />
powdery mildew fungus (PM), Erysiphe necator (Schw.) Burr., in Vitis vinifera ‘Cabernet<br />
Sauvignon’ <strong>and</strong> Vitis aestivalis ‘Norton’. We found that transcript levels of two STS genes<br />
increased along the berry development <strong>and</strong> were significantly higher in Cabernet Sauvignon than<br />
in Norton. Transcript levels of other three STS genes increased during the berry ripening, <strong>and</strong><br />
were higher in Norton than in Cabernet Sauvignon. In response to PM infection, four STS genes<br />
were up-regulated in Cabernet Sauvignon leaves while three STS genes were induced in the<br />
Norton leaves. These results demonstrated that each STS gene plays a special role in the berry<br />
development <strong>and</strong> in the defense against fungal infection, <strong>and</strong> revealed new insights into the<br />
regulation of the STS gene family in grapevine.<br />
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