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P-111 Cloning and functional characterisation of a putative powdery mildew susceptibility gene in grapevine A.S. Salami 1 , A. Ebadi 1 , M.R. Naghavi 1 , I. Dry 2 * 1 Faculty of Agriculture and Natural Sciences, University of Tehran, Tehran, Iran; 2 CSIRO Plant Industry, Waite Campus, Urrbrae, South Australia, Australia *Corresponding author: ian.dry@csiro.au Grapevine is highly susceptible to a range of different fungal pathogens including powdery mildew (Erysiphe necator), which can cause severe reductions in yield and quality. As part of multifaceted approach to improve the genetic resistance of grapevine to powdery mildew, we are investigating the grapevine pectate lyase-like (PLL) gene family because previous research in Arabidopsis has demonstrated that a specific member of the AtPLL gene family (PMR6) was required for powdery mildew susceptibility. Sixteen VvPLLs were predicted to be present in the grapevine genome. Based on sequence homology, we identified three genes, designated VvPLL1, VvPLL2 and VvPLL3, as potential orthologs of PMR6, and the coding regions were amplified from Vitis vinifera ‘Cabernet sauvignon’. All three VvPLL candidates were found to encode proteins with a C-terminal GPI-anchor, which is a characteristic of PMR6 and VvPLL-GFP fusion constructs confirmed the VvPLL proteins were targeted to the plasma membrane. Quantitative RT-PCR analysis indicated constitutive expression of VvPLL1, VvPLL2 and VvPLL3 in all tissues examined including leaves, roots, flowers and berries. In contrast, other members of the VvPLL gene family such as VvPLL9 and VvPLL10 were tissue-specific (floral). In agreement with observations for PMR6, VvPLL1, VvPLL2 and VvPLL3 were not significantly up-regulated by powdery mildew infection. Functional complementation experiments are currently underway in which the three VvPLL genes have been transformed into the Atpmr6 mutant under the control of either the CaMV 35S or Arabidopsis PMR6 promoter. Our ultimate aim is to silence these VvPLL genes in grapevine to determine if we can modify susceptibility to powdery mildew. 191 
P-112 Evaluation of grapevine germplasm from La Rioja for tolerance to powdery mildew (Erisiphe necator) M.M. Hernandez 1 , S. Song 1,2 , C. Menéndez* 1 1 Departamento de Agricultura y Alimentación. Universidad de La Rioja. Instituto de Ciencias de la Vid y el Vino. UR-CSIC-GR., Logroño, Spain; 2 College of Enology, Northwest Agriculture & Forestry University, China *Corresponding author: cristina.menendez@unirioja.es Powdery mildew is caused by the plant pathogenic fungus Erisiphe necator and is one of the most important and destructive diseases of grapevine in many countries of the world, including Spain. Development and use of tolerant varieties is one of the most important and environmentally sound strategies for management of this disease. Susceptibility or tolerance to powdery mildew infection were evaluated on shoots in 49 accessions of the germplasm collection from an experimental field at La Rioja University (Spain). The natural infections were scored during 2008-2009 using a six point scale. No pesticides were used in the experimental sites, and a high severity of infection was observed, with all accessions infected by the fungus but showing differences in the severity levels. The more tolerant were R-14, SO-63, CI-78 and the more susceptible accessions were H-29, AR-43, B-47 and AB-100. The results were promising as they may be used as a tolerant genetic sources for breeding to manage the disease. 192 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
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P-8 Monoterpene levels in different
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P-10 Usefulness of the SCC8 scar ma
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P-12 The onset of berry ripening is
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P-14 Morphological characteristics
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P-16 A genetic analysis of berry qu
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P-18 Use of molecular markers for s
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P-20 Characterization of key compon
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P-22 Expression of early light-indu
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P-24 The development of molecular m
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P-26 Identification of ABA inducibl
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P-28 In vitro selection of HYP tole
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P-30 In silico SNP detection for ge
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P-32 Vitis vinifera genome annotati
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P-34 Genome-wide identification of
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P-36 Looking for genetic polymorphi
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P-38 Characterization of a new horm
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P-40 Towards a characterization of
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the overall identified polymorphism
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P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
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P-48 ‘Cioutat’: a somatic varia
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P-50 Estimation of protein spot var
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glucosyltransferase, anthocyanidin
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P-53 Marker-free transgenic grapevi
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P-55 Agrobacterium rhizogenes-media
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P-57 Phenotypic evaluation of ‘Th
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P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175 and 176: P-95 Reaction of grape rootstocks t
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179 and 180: P-99 Berry cracking caused powdery
Page 181 and 182: P-101 Elicitation of grapevine defe
Page 183 and 184: P-103 The biological characteristic
Page 185 and 186: P-105 Pathogen responsiveness and v
Page 187 and 188: P-107 Screening grape hybrid famili
Page 189: P-109 Characterization of an ankyri
Page 193 and 194: P-114 Evaluation of four new rootst
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