conference schedule and program with abstracts - Horticulture ...
conference schedule and program with abstracts - Horticulture ...
conference schedule and program with abstracts - Horticulture ...
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P-2<br />
PMEI <strong>and</strong> SPY: Two genes <strong>with</strong> possible roles in seed <strong>and</strong> berry development in table<br />
grape (Vitis vinifera L.)<br />
M. González-Agüero 1,2* , P. Muñoz-Robredo 3 , O. Gudenschwager 1 , X.C. Iommi 4* , P. Hinrichsen 1<br />
1<br />
Instituto de Investigaciones Agropecuarias (INIA) – CRI La Platina; 2 The Plant Cell<br />
Biotechnology Millennium Nucleus (PCB-MN); 3 Facultad de Ciencias Físicas y Matemáticas,<br />
Departamento de Ingeniería Química y Biotecnología, Universidad de Chile; 4 Universidad<br />
Andres Bello, Chile<br />
*Corresponding author: xcasanueva@gmail.com<br />
Table grape (Vitis vinifera L.) is the most important species exported by the Chilean fruit<br />
industry. Seedlessness <strong>and</strong> berry size are important quality parameters for fresh consumption.<br />
Although progress has been made in underst<strong>and</strong>ing the molecular basis of stenospermocarpy, the<br />
biochemical <strong>and</strong> molecular basis underlying this process are poorly understood. To contribute to<br />
this underst<strong>and</strong>ing, we characterized <strong>and</strong> analyzed the expression of two genes, PMEI<br />
(Pectimethylesterase Inhibitor) <strong>and</strong> SPY (Spindly), which are strongly related to the seedless<br />
condition. Both genes were obtained by quantitative trait locus (QTL) mapping using a reference<br />
population originated from a 'Ruby Seedless' x 'Thompson Seedless' cross, which included<br />
contrasting phenotypes, i.e. seeded <strong>and</strong> seedless segregants, <strong>with</strong> large <strong>and</strong> small berries. We<br />
cloned <strong>and</strong> characterized the expression pattern of these genes in Vitis. The expression profile<br />
was characterized by -real time PCR <strong>and</strong> the amplification assays were performed in seven<br />
different stages of development, in four contrasting phenotypes (three individuals each) related<br />
to seed <strong>and</strong> berry development. As berry development progressed, a change in the levels of<br />
transcript was observed for most of the analyzed genes. The significance of these changes in<br />
expression will be discussed.<br />
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