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P-33 Analysis of expressed sequence tags (EST) from Vitis vinifera L. flower and fruit and electronic mapping of new ESTs J. Fang*, L. Shangguan, C. Song, C. Wang, N.K. Korir, G. Yang College of Horticulture, Nanjing Agricultural University, Nanjing, China *Presenting author: fanggg@njau.edu.cn Expressed Sequence Tags (EST) are a cost-effective molecular biology tool that eases our understanding of genes involved in plant growth and differentiation. ESTs represent a valuable and abundant resource for genome annotation, gene expression, and comparative genomics in plants. In this study, a total of 6,230 EST sequences were produced from 7,561 clones in the grape (Vitis vinifera cv. Summer Black) flower and fruit cDNA library. After cluster and assembly of datasets, 3,582 unigenes comprising of 1,070 contigs and 2,512 singlets were established. 925 unique ESTs with known or putative functions were gained by BlastX analysis against the NCBI non-redundant protein database (nr). The sequences were assigned to 12 putative cellular roles based on additional functional annotation as well as comparative and classification analysis. 381 new ESTs were gained after Blastn analysis, and 79 SSR loci were mined from these new ESTs by MISA. After electronic mapping, 289 new EST sequences could be mapped to all the 19 grape chromosomes. The three highest matching chromosomes (chr) were chr18, chr5 and chr14, with 11.76% (34), 8.30% (24) and 7.96% (23) new ESTs mapped on, respectively. 109 
P-34 Genome-wide identification of grapevine specific genes B. Cai 1,2 , J. Tao 1 , Z. Zhang 1 , Z-M. Cheng* 1,2 1 College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province, China; 2 Department of Plant Sciences, University of Tennessee, Knoxville, Tennessee, USA *Corresponding author: zcheng@utk.edu Using the grapevine (Vitis vinifera) genome annotation, along with the eight sequenced genomes (Arabidopsis, Oryza, Populus, Carica, Glycine, Medicago, Sorghum, and Zea), the plant transcript assemblies from 250 plant species, ESTs and no redundant (NR) proteins datasets from GenBank, we identified 1,859 genes in grapevine that lack significant sequence similarity with all other species. This set of 1,859 genes is termed grapevine-specific genes (GSGs). These GSGs were screened against the entire grapevine ESTs from NCBI to identify expressed genes. We obtained 96 expressed GSGs that have the corresponding ESTs in GenBank. For the expressed GSGs, the in silico gene expression, protein motif were examined. The expressed GSGs set were divided into five groups and eight protein motifs were identified by the MEME program from the five group sequences. These genes may play roles in determining specific characteristics of grapevines. 110 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
Page 57 and 58: O-38 Co-Localization of QTLs for Se
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107: P-32 Vitis vinifera genome annotati
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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