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P-47 Grape berry cuticle under water deficits: morphological, metabolomic and proteomic analysis O. Zarrouk* 1 , R. Francisco 1 , R.R. Santos 1 , J.M. Costa 1,2 , V. Fernandez 3 , M.M. Chaves 1 1 Instituto de Tecnologia Química e Biológica, Oeiras, Universidade Nova de Lisboa, Portugal; 2 Instituto Superior de Agronomia, Lisboa, Portugal; 3 Estación Experimental de Aula Dei (CSIC). Zaragoza, Spain *Corresponding author: zolfa@itqb.unl.pt The cuticle membrane is an essential structure of high functional and ecological importance and is involved in the development of some disorders in skin of fruits. The cuticle restricts water loss between the epidermis and adjacent environment of the plant and protects against nutrient leaching, mechanical damage, and invasion by pathogens. Fruit cuticle composition influences berry weight loss during maturation and may determine the efficacy of alcoholic fermentative process during wine making, as it is 30% composed by the cyclic terpenoid oleanolic acid, a compound known to be important in such processes. Despite the importantce of fruit cuticles, little is known about its synthesis, assembly and metabolism. Recent reports show that cuticular wax deposition and composition influences drought tolerance. In addition, the lack of some flavonoids compounds was associated with alterations in cuticle thickness, cutin content and wax composition in tomato fruit cuticle. Combined with metabolic and morphological characterization, proteomics will provide a quantitative characterization of protein dynamics in response to water deficit and contribute to the identification of potentially biological players involved in the transport, deposition or modification of the cuticle. We studied the berry cuticle in three phenological stages (pea size, véraison and full maturation) under two water irrigation conditions (fully irrigated and non irrigated grapevines) by analyzing cuticle flavonoid composition, proteome and morphology. Berry skins from non-irrigated grapevines showed a different morphology, composition and more epicuticular waxes than those from fully irrigated ones. 125 
P-48 ‘Cioutat’: a somatic variant of ‘Chasselas’ exhibiting a programmed cell death-like phenotype D. Lijavetzky 1 *, L. Fernandez, J. Diaz-Riquelme, J-M. Martinez-Zapater 1 Instituto de Biologia Agricola de Mendoza (IBAM), CONICET/FCA-UNCuyo, Mendoza, Argentina *Corresponding author: diegolija@hotmail.com Somatic genetic variation constitutes an important source of phenotypic variation in grapevine. In perennial plant species where mutants are difficult to generate and screen, spontaneous somatic variants represent a unique resource to understand the genetic control of complex developmental patterns. ‘Cioutat’ is somatic variant of cultivar ‘Chasselas’ displaying parsleylike leaves. Genetic analysis of progeny of a ‘Cioutat’ self-cross population showed 25% plants with wild-type (‘Chasselas’) phenotype, consistent with the hypothesis that the phenotype is caused by a single dominant mutation. Transcriptomic comparison of ‘Cioutat’ and ‘Chasselas’ revealed significant over-expression of a BAG (Bcl-2 associated athanogene) gene closely related to the Arabidopsis AtBAG6 gene in the ‘Cioutat’ leaf primordia. We named that grapevine gene as VvBAG6. Interestingly, in Arabidopsis, AtBAG6 is suggested to be a stress upregulated protein involved in programmed cell death (PCD). Moreover, a quantitative evaluation of the ‘Cioutat’ phenotype in the self-cross segregant population showed a positive and statistically significant correlation between VvBAG6 expression level and the extension of the parsley leaf phenotype evaluated in the same plants. Collectively, these results suggest the involvement of PCD in the generation of the ‘Cioutat’ phenotype. 126 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123: grapevine which will eventually be
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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