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O-32 Integrating ecophysiology and quantitative genetics to analyse the control of transpiration by the rootstock under drought conditions E. Marguerit*, C. Van Leeuwen, S. Delrot, N. Ollat UMR “Ecophysiology and Functional Genomic of Grapevine” INRA, University of Bordeaux, ENITA, ISVV, Villenave d’Ornon, France *Corresponding author: e-marguerit@enitab.fr Water is the main limiting factor for yield in viticulture. Vine water status also strongly impacts grape quality. The objective of this work is to analyze the genetic determinism of drought responses induced by the rootstock. The mapping pedigree used consisted of 138 F1 individuals derived from the interspecific cross of V. vinifera ‘Cabernet Sauvignon’ (CS) × V. riparia ‘Gloire de Montpellier’ (RGM). ‘Cabernet Sauvignon’ was the scion grafted on each rootstock of this population. The experiment was carried out in pots, in a greenhouse. Water retention properties of the substrate were primarily determined. Transpiration was evaluated daily by weighing each pot individually with a 150 scale platform. Irrigation was applied in the mid morning in order to compensate exactly for the difference between the daily water loss due to transpiration in a particular pot and the loss of water calculated to obtain the desired level of water stress. Leaf area measurements were performed weekly in order to calculate the daily transpiration per unit of leaf area. After ten days without any stress, progressive water limitation was applied for ten days and followed by a stable water deficit stress for 15 days. These phenotypic measurements were recorded in 2007, 2008, and 2009. The fraction of transpirable soil water (FTSW) was used to define the intensity of the water stress. The response curves of transpiration to FTSW in each pot were assessed and mathematically adjusted. A large variability was observed in the studied population. QTL analysis was then performed with the coefficients of transpiration response curves. The inflexion point of transpiration response curve to FTSW was used as a plasticity trait of transpiration regulation. MultiQTL software permitted us to consider statistically the three years of investigation. Stable QTLs were detected on four linkage groups. These results demonstrate that transpiration regulation of the scion by the rootstock is determined genetically. This is the first genetic quantitative study taking into account transpiration plasticity to evaluate the water stress tolerance. These results could improve the understanding of the interaction relation between the scion and the rootstock. 53 
O-34 Cloning and characterization of the dagger nematode resistance gene XiR1 C-F. Hwang* 1 , K. Xu 2 , R. Hu 3 , S. Riaz 3 , M.A. Walker 3 1 William H. Darr School of Agriculture, Missouri State University, Mountain Grove, Missouri, United States; 2 Department of Horticultural Sciences, Cornell University, Geneva, New York, United States; 3 Department of Viticulture and Enology, University of California, Davis, California, United States *Corresponding author: cfhwang@ucdavis.edu The dagger nematode, Xiphinema index, feeds aggressively on grape roots and in the process vectors grapevine fanleaf virus (GFLV) leading to the severe viral disease known as fanleaf degeneration. Disease symptoms consist of disrupted fruit set caused by GFLV and depressed plant growth caused by root damage from X. index. The use of fumigants to control X. index in vineyards is no longer recommended because of their high cost, detrimental environmental effects and lack of effective soil penetration to control nematodes on a deep perennial root system. Therefore, resistance to X. index has been an important objective in grape rootstock breeding programs. We previously demonstrated that resistance to X. index derived from a Vitis arizonica/girdiana hybrid b42-26 was largely controlled by a major quantitative trait locus, XiR1 (X. index Resistance 1) located on chromosome 19. Genetic studies leading to the isolation and characterization of the genes conferring resistance to X. index would further our understanding of resistance and assist molecular and classical breeding efforts to control X. index. In this report, we present the development of high resolution genetic and physical maps in the XiR1 region as well as the isolation of the XiR1 locus by a positional cloning approach. This study has identified the first locus responsible for ectoparasitic nematode resistance. The markers developed from this study are being used to expedite the breeding of resistant grape rootstocks. 54 
Page 1 and 2: Date Time Session Topic Sunday Augu
Page 3 and 4: Monday August 2, 2010 Opening Plena
Page 5 and 6: Topic: Adaptation to soils and clim
Page 7 and 8: Tuesday August 3, 2010 Oral Session
Page 9 and 10: Poster Session 2 1:30 PM-3:00 PM Po
Page 11 and 12: P-105 Pathogen responsiveness and v
Page 13 and 14: Table of Contents Keynote Presentat
Page 15 and 16: K-2 Progress in grapevine breeding
Page 17 and 18: K-4 Genetics and Genomics of Grapev
Page 19 and 20: O-2 Additional homologs of the Ren1
Page 21 and 22: O-4 Y. Xu and Y.Wang* Introduction
Page 23 and 24: O-5 Identification of an R2R3 MYB t
Page 25 and 26: O-7 Sequencing of the phylloxera re
Page 27 and 28: O-9 Dissection of defense pathways
Page 29 and 30: O-11 Recent trends and technologies
Page 31 and 32: O-13 Brazilian Grape Breeding Progr
Page 33 and 34: O-15 Development of rootstocks for
Page 35 and 36: O-17 L. Wang* 1 , S. Li 1, 2 , P. F
Page 37 and 38: O-19 Integrated analysis of transcr
Page 39 and 40: O-20 Deciphering the chromosome str
Page 41 and 42: O-22 Identification of table grapes
Page 43 and 44: O-24 Metagenomic sequencing as a to
Page 45 and 46: O-26 Regulation of bud fruitfulness
Page 47 and 48: O-28 Haplotype structure and cluste
Page 49 and 50: O-30 Annette Nassuth Function of Vi
Page 51: 10 to 25% of the chlorotic symptoms
Page 55 and 56: O-36 New insight into the genetics
Page 57 and 58: O-38 Co-Localization of QTLs for Se
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104:
P-28 In vitro selection of HYP tole
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P-30 In silico SNP detection for ge
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P-32 Vitis vinifera genome annotati
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P-34 Genome-wide identification of
Page 111 and 112:
P-36 Looking for genetic polymorphi
Page 113 and 114:
P-38 Characterization of a new horm
Page 115 and 116:
P-40 Towards a characterization of
Page 117 and 118:
the overall identified polymorphism
Page 119 and 120:
P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
Page 125 and 126:
P-48 ‘Cioutat’: a somatic varia
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P-50 Estimation of protein spot var
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glucosyltransferase, anthocyanidin
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P-53 Marker-free transgenic grapevi
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P-55 Agrobacterium rhizogenes-media
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P-57 Phenotypic evaluation of ‘Th
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P-59 Phenotypic divergence among gr
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P-61 Grepevine variety determinatio
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agricultural context. This network
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P-64 Genetic structure in a large r
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P-66 Analysis of grape rootstocks b
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P-68 Italian wild grapevine: a stat
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Rebula-Robola group were genotyped
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P-71 Viticultural performance of so
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P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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