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P-11 Drawing links from transcriptome to metabolites: the evolution of Muscat aroma in the ripening berry L. Costantini* 1 , J. Battilana 1 , C. Kappel 2 , F. Emanuelli 1 , M. Sordo 1 , S. Delrot 2 , M.S. Grando 1 1 IASMA Research Centre, Fondazione Edmund Mach, San Michele all’Adige, Trento, Italy; 2 UMR Ecophysiology and Grape Functional Genomics, University of Bordeaux, INRA, Institut des Sciences de la Vigne et du Vin, Villenave d’Ornon, France *Corresponding author : laura.costantini@iasma.it Owing to the role that aroma plays in the perceived quality of grapes and wines, there is a tremendous interest in understanding how the accumulation of sensory compounds is regulated at the molecular level. To this purpose, we applied an integrative functional genomics approach by simultaneously monitoring the abundance of transcripts and metabolites in ripening berries of Moscato Bianco (Vitis vinifera L.).Gas chromatography-mass spectrometry analysis of thirty-two out of the most important ‘impact’ compounds (monoterpenoids, C 13 -norisoprenoids, benzenoids and C 6 -aliphatic compounds) in their free and bound forms was combined with a large-scale transcriptome analysis based on the use of the AROS 1.0 oligonucleotide microarray (14,562 probes) at five developmental stages from pre-véraison to over-ripening. Several exploratory methods were tested for the integration of transcript and metabolite profiles with the final goal to identify genes or groups of genes which are linked with the accumulation of specific metabolites or groups of metabolites. Pair-wise metabolite-to-metabolite and gene-to-metabolite correlations were calculated. Various types of clustering were performed by incorporating both differentially expressed genes and metabolites. Gene network analysis was assayed in order to identify items with similar patterns of co-regulation. Finally, modulated transcripts were checked for their potential co-localization with previously identified QTLs controlling the level of aromatic compounds. This work is expected to provide insight into the regulatory network of grapevine secondary metabolism and is aimed at identifying genes relevant to aroma determination both at the regulatory and catalytic levels. 87 
P-12 The onset of berry ripening is characterized by hydrogen peroxide accumulation and lipids peroxidation S. Pilati 1 , R. Frassanito 2 , G. Guella 2 , C. Moser* 1 1 IASMA Research and Innovation Center, Fondazione Edmund Mach, San Michele a/Adige (TN), Italy; 2 Bioorganic Chemistry University of Trento, Povo (TN), Italy *Corresponding author: claudio.moser@iasma.it The process of grape berry ripening is very complex and it involves the fine regulation of many different metabolic pathways. Recent analyses revealed that the process is significantly regulated at the transcriptional level and that véraison is a key regulatory moment. It is known that hormones such as abscissic acid, auxins, brassinosteroids and ethylene play an essential role in the coordination of the whole berry ripening process. We have recently reported a strong accumulation of hydrogen peroxide (H 2 O 2 ) in Pinot Noir grapes around vèraison. The occurrence of an oxidative stress during fruit development, around the time of color change, has been observed in several other fruits (tomato, pear, raspberry, strawberry). We are currently engaged in understanding if the observed peak of ROS in the grapes is part of the ripening regulatory network. In this paper an update of our current view of this process will be given. Our results showed that in berry skin H 2 O 2 accumulation is accompanied by lipid peroxidation and an increase in catalase activity. MALDI-TOF mass spectrometry of the lipid fraction indicated a site-specific peroxidation of the galactolipids, which are mainly present in the chloroplasts. Preliminary results suggest that a lipoxygenase, which accumulate at véraison, would be involved in the peroxidation process. - 88 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
Page 35 and 36: O-17 L. Wang* 1 , S. Li 1, 2 , P. F
Page 37 and 38: O-19 Integrated analysis of transcr
Page 39 and 40: O-20 Deciphering the chromosome str
Page 41 and 42: O-22 Identification of table grapes
Page 43 and 44: O-24 Metagenomic sequencing as a to
Page 45 and 46: O-26 Regulation of bud fruitfulness
Page 47 and 48: O-28 Haplotype structure and cluste
Page 49 and 50: O-30 Annette Nassuth Function of Vi
Page 51 and 52: 10 to 25% of the chlorotic symptoms
Page 53 and 54: O-34 Cloning and characterization o
Page 55 and 56: O-36 New insight into the genetics
Page 57 and 58: O-38 Co-Localization of QTLs for Se
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85: P-10 Usefulness of the SCC8 scar ma
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
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P-59 Phenotypic divergence among gr
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P-61 Grepevine variety determinatio
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agricultural context. This network
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P-64 Genetic structure in a large r
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P-66 Analysis of grape rootstocks b
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P-68 Italian wild grapevine: a stat
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Rebula-Robola group were genotyped
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P-71 Viticultural performance of so
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P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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