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P-21 Anthocyanin Profiling in the Berry Skins of Five Vitis amurensis Grapes and One Related Hybrid Cultivar F. He* and J. Wang Centre for Viticulture and Enology, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, P.R. China. *Corresponding author: wheyfey@yahoo.com.cn V. amurensis is the world’s most northerly grapevine species that originates in the exceptionally cold climate in northeast China, and many V. amurensis grapes have been widely used because of not only their strong cold tolerance and disease hardness, but also high pigment content. However, until now it remains no detailed description of the anthocyanin composition and content in V. amurensis grapes. In this study, anthocyanin profiles in the berry skins of five V. amurensis grapes and one hybrid cultivar were analyzed by high performance liquid chromatography-electronic spray ionization-tandem mass (HPLC-ESI-MS/MS). The result showed that both the V. amurensis grapes and the hybrid cultivar contained 3,5-diglucosides of anthocyanidins with high amount in addition to 3-monoglucosides of delphinidin, cyanidin, petunidin, peonidin and malvidin that are generally detected in V. vinifera grapes. Furthermore, pelargonidin-3, 5-diglucoside and B-type vitisins of some anthocyanidin-3-glucosides were also identified in trace amount and no acetylated anthocyanins were detected in these samples. This finding will be important to utilize this germplasm resource in grapevine breeding programs and to further uncover biosynthesis and regulation of anthocyanins in V. amurensis grapes. 97 
P-22 Expression of early light-inducible proteins (ELIPs) during the transition to autotrophy of Vitis vinifera L. leaves D.E. Olivares* 1,2 , E. Sánchez 1 , R. Pulgar 2 , W. Sierralta 3 , P. Hinrichsen 1 , M. Pinto 1,2 1 Instituto de Investigaciones Agropecuarias, Santiago, Chile; 2 Facultad de Ciencias Agronómicas, Universidad de Chile, Santiago, Chile; 3 Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago, Chile *Corresponding author: olivaresdaniela@gmail.com ELIPs are thylakoid membrane proteins transiently induced by light and particularly expressed following photoinhibition. They belong to the chlorophyll a/b binding protein family and are structurally related to the light harvesting complex of photosystem II. Their function is not totally understood - some authors associate ELIPs to energy dissipation processes but others gives them a role on the protection of chlorophyll again degradation. ELIPs would bind the molecules of free chlorophyll acting as pigment carriers during plastid development. Since accumulation of chlorophyll is normally occurring in developing leaves and in grapevine young leaves normally develop under photoinhibitory conditions, in this work we analyzed the expression of ELIPs in grapevine leaves and its relationship with the rate of chlorophyll accumulation occurring during leaf transition to the autotrophy. Our results revealed that in grapevine leaves only one ELIP (VvELIP) was expressed, which would be encoded by a singlecopy gene that maps to Vitis vinifera chromosome 5. It was also determined that the promoter of this gene presents a structure with cis-acting regulatory sequences induced by light and express following a circadian rhythm. This was corroborated by the oscillatory expression of the elip transcript in leaves kept under continuous illumination but previously acclimated to a 12 h photoperiod. VvELIP corresponds to a typical early light inducible protein with 199 amino acid, a 44 amino acid long chloroplast transit peptide, three transmembrane helices and a chlorophyll a/b-binding domain. After high light induction, VvELIP was highly expressed in heterotrophic leaves but very little in adult autotrophic leaves. A positive correlation was found between the level of VvELIP expression and the rate of chlorophyll accumulation. These results are in agreement with the chlorophyll protection role assigned to ELIPs which can be of importance during transition of leaves to autotrophy. 98 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
Page 45 and 46: O-26 Regulation of bud fruitfulness
Page 47 and 48: O-28 Haplotype structure and cluste
Page 49 and 50: O-30 Annette Nassuth Function of Vi
Page 51 and 52: 10 to 25% of the chlorotic symptoms
Page 53 and 54: O-34 Cloning and characterization o
Page 55 and 56: O-36 New insight into the genetics
Page 57 and 58: O-38 Co-Localization of QTLs for Se
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95: P-20 Characterization of key compon
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
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P-68 Italian wild grapevine: a stat
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Rebula-Robola group were genotyped
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P-71 Viticultural performance of so
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P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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