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P-104 Development of marker-assisted selection as a tool for breeding disease resistant grapes B.I. Reisch 1* , S. Mahanil 2 , N. Consolie 2 , R.S. Luce 1 , P.G. Wallace 1 , L. Cadle-Davidson 2 1 Department of Horticultural Sciences, Cornell University, NYS Agric. Expt. Station, Geneva, New York, USA; 2 USDA-ARS Grape Genetics Research Unit, Geneva, New York, USA *Corresponding author: bir1@cornell.edu The Cornell grape breeding program has a long-standing effort to develop disease resistant grape cultivars. A number of molecular markers that are closely associated with disease resistance loci have been identified. We sought to examine a range of molecular markers for resistance loci and determine their utility in populations already in use in the grape breeding program. Populations of seedlings were screened in the laboratory for downy mildew resistance using detached leaves. In a field nursery, populations were screened for resistance to powdery mildew. Field grown seedlings potentially carrying both the Rpv1 and Run1 loci for downy and powdery mildew resistance, respectively, from muscadine (Vitis rotundifolia) introgressions were also assessed for resistance to both mildews. In these populations, markers for the Run1 gene were excellent predictors of powdery mildew resistance, but markers for the Rpv1 gene for downy mildew resistance from the muscadine grape were not useful in predicting downy mildew resistance. Markers for the V. cinerea source of powdery mildew resistance were also found to be useful. Populations that were previously screened for powdery mildew resistance (where the susceptible types had already been discarded) had a very high frequency of markers for the Run1 gene and/or the V. cinerea source of resistance, confirming the success of field screening techniques. By screening for presence of markers, it was possible to identify seedlings harboring both the V. cinerea as well as muscadine sources of powdery mildew resistance. 185 
P-105 Pathogen responsiveness and variety/tissue specificity of five stilbene synthase genes in grapevine R. Dai*, M.B. Ali, W. Qiu Center for Grapevine Biotechnology, William H. Darr School of Agriculture, Missouri State University, Mountain Grove, Missouri, USA *Corresponding author: ru0201@live.missouristate.edu Grapevine stilbenic compounds have antimicrobial activities and some of them including resveratrol are beneficial to human health. Stilbene synthase (STS) is the key enzyme that catalyzes the biosynthesis of stilbenic compounds. Grapevine genome contains 43 STS genes, and the regulation of each STS gene needs to be understood for defining their roles. In this study, we selected five STS genes and employed quantitative polymerase chain reaction (qPCR) to characterize their transcriptional profiles during the berry development and infection by the powdery mildew fungus (PM), Erysiphe necator (Schw.) Burr., in Vitis vinifera ‘Cabernet Sauvignon’ and Vitis aestivalis ‘Norton’. We found that transcript levels of two STS genes increased along the berry development and were significantly higher in Cabernet Sauvignon than in Norton. Transcript levels of other three STS genes increased during the berry ripening, and were higher in Norton than in Cabernet Sauvignon. In response to PM infection, four STS genes were up-regulated in Cabernet Sauvignon leaves while three STS genes were induced in the Norton leaves. These results demonstrated that each STS gene plays a special role in the berry development and in the defense against fungal infection, and revealed new insights into the regulation of the STS gene family in grapevine. 186 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
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P-8 Monoterpene levels in different
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P-10 Usefulness of the SCC8 scar ma
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P-12 The onset of berry ripening is
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P-14 Morphological characteristics
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P-16 A genetic analysis of berry qu
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P-18 Use of molecular markers for s
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P-20 Characterization of key compon
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P-22 Expression of early light-indu
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P-24 The development of molecular m
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P-26 Identification of ABA inducibl
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P-28 In vitro selection of HYP tole
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P-30 In silico SNP detection for ge
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P-32 Vitis vinifera genome annotati
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P-34 Genome-wide identification of
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P-36 Looking for genetic polymorphi
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P-38 Characterization of a new horm
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P-40 Towards a characterization of
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the overall identified polymorphism
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P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
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P-48 ‘Cioutat’: a somatic varia
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P-50 Estimation of protein spot var
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glucosyltransferase, anthocyanidin
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P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175 and 176: P-95 Reaction of grape rootstocks t
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179 and 180: P-99 Berry cracking caused powdery
Page 181 and 182: P-101 Elicitation of grapevine defe
Page 183: P-103 The biological characteristic
Page 187 and 188: P-107 Screening grape hybrid famili
Page 189 and 190: P-109 Characterization of an ankyri
Page 191 and 192: P-112 Evaluation of grapevine germp
Page 193 and 194: P-114 Evaluation of four new rootst
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