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P-65 Plastid DNA sequence diversity in a worldwide set of grapevine cultivars (Vitis vinifera L. subsp. vinifera) B. Schaal 1 , J. Beck 1 , S-C. Hsu 1 , T. Beridze* 2,3 , M. Gamkrelidze 2 , M. Gogniashvili 2 , I. Pipia 2 , V. Tabidze 2 , P. This 4 , R. Bacillieri 4 , V. Gotsiridze 5 , M. Glonti 5 1 Washington University, St. Louis, Missouri; 2 Durmishidze Institute of Biochemistry and Biotechnology, Tbilisi, Georgia; 3 Ilia State University, Tbilisi, Georgia ; 4 Institut National de la Recherche Agronomique (INRA) Montpellier, France ; 6 Institute of Horticulture, Viticulture and Oenology, Tbilisi, Georgia *Corresponding author: tgberidze@yahoo.com, tengiz_beridze@iliauni.edu.ge DNA sequence diversity was investigated at two plastid regions (the trnH-psbA intergenic spacer and the rpl16 intron) in a geographically diverse group of 113 cultivated grape samples. This group included 40 samples from the Republic of Georgia, home to over 500 grape cultivars and the earliest archaeological evidence of grape domestication. The greater Caucasus region in which Georgia lies is widely believed to be the area in which grape domestication began, and the study of genetic diversity in this region is viewed as key to understanding grape domestication in general. Four plastid haplotypes are evident in the 113 samples, and are designated by their character-states at each of the 3 polymorphic positions: (AAA)–23 samples, (ATT)–29 samples, (GTA)–34 samples, and (ATA)–27 samples. The AAA haplotype was only observed in Georgian samples, and these 23 “Rkatsiteli” group cultivars originate mostly from eastern Georgia. Contrast this group with the nine Georgian cultivars (23%) of the “Chkhaveri-Pinot noir” group (GTA), most of which are cultivated in western Georgia near the Black Sea coast. The observation that the Georgian cultivars exhibited both unique plastid DNA variation (the AAA haplotype) and all other observed plastid haplotypes is consistent with previous studies that have observed both unique and high levels of genetic variation in wild grape (V. vinifera subsp. sylvestris) in the greater Caucasus region. 145 
P-66 Analysis of grape rootstocks by microsatellite markers G. Jahnke* 1 , G. Kocsisne Molnar 2 , J. Majer 1 , B. Szoke 1 , E. Tarczal 2 , P. Varga 1 , L. Kocsis 2 1 Research Institute for Viticulture and Oenology, Centre of Agricultural Sciences, University of Pannonia, Badacsony, Hungary; 2 Department of Horticulture, University of Pannonia, Georgikon Faculty, Keszthely, Hungary *Corresponding author: gjahnke@mail.iif.hu The aim of this work was to determine the SSR profile of 96 Vitis accessions (mainly rootstocks) at 19 loci and to find genetic relatedness between them. Molecular markers used in the last 30 years are not effected by the environment, giving a good tool for determination of genetic distances. Based on our former experiences SSR analysis was carried out on 96 grape accessions, mainly rootstocks. DNA was extracted from young leaves. Analyses were carried out using 19 microsatellite loci. The loci were selected to cover all of the linkage groups to get more exact impression of the genome of the grape rootstocks. Results of SSR analysis and a dendogram showing genetic relatedness were constructed. Based on the results it can be established that most of the loci showed appropriate polymorphism. High similarity was detected between the clones of T5C and TK5BB. Strong differences were detected between the so called “clones” of T5C and TK5BB, suggesting, that they are not real clones, but different genotypes with highly similar morphological features. The Vitis sylvestris accessions showed close relatedness with the Vitis vinifera L. varieties. 146 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
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P-8 Monoterpene levels in different
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P-10 Usefulness of the SCC8 scar ma
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P-12 The onset of berry ripening is
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P-14 Morphological characteristics
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P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143: P-64 Genetic structure in a large r
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175 and 176: P-95 Reaction of grape rootstocks t
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179 and 180: P-99 Berry cracking caused powdery
Page 181 and 182: P-101 Elicitation of grapevine defe
Page 183 and 184: P-103 The biological characteristic
Page 185 and 186: P-105 Pathogen responsiveness and v
Page 187 and 188: P-107 Screening grape hybrid famili
Page 189 and 190: P-109 Characterization of an ankyri
Page 191 and 192: P-112 Evaluation of grapevine germp
Page 193 and 194: P-114 Evaluation of four new rootst
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