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O-39 Polymorphisms in VvPel associate with variation for berry texture A.M. Vargas 1 , M.T. de Andrés, J. Ibáñez* 2 Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario. Finca El Encín, Alcalá de Henares, Spain 1 Present address: Centro de Biotecnología y Genómica de Plantas (CBGP) INIA-UPM. Campus de Montegancedo, Pozuelo de Alarcón, Spain; 2 Present address: Instituto de Ciencias de la Vid y del Vino. Complejo Científico Tecnológico, Logroño, Spain *Corresponding author: javier.ibanez@icvv.es The identification of the genes involved in traits of interest, and the understanding of its interaction with other genetic or environmental factors, are among the main aims of genetic studies on cultured plants today. Berry texture is a quality trait of great importance in table grapes, where ‘crunchy’ berries are especially appreciated. Nevertheless, no QTL or related gene has been described for this trait so far. In this work, the gene VvPel, which codes for a pectate lyase in grapevine, was selected as a candidate gene to carry out an association study. Pectate lyase is a cell wall degrading enzyme that catalyses the eliminative cleavage of de-esterified pectin, which is a major component of the primary cell walls of many higher plants. The gene was chosen because previous studies in other species have reported a relation between this enzyme and fruit softening. Different analyses were carried out to complement the association test: the population structure, the nucleotide and haplotype diversity, the protein structure, the existence of selection and the linkage disequilibrium (LD) were evaluated in the gene VvPel. Firstly, a core collection of 96 table grape accessions was built from a collection of 322 table grape accessions maintained in the Finca El Encín (IMIDRA), using a reiterated maximization procedure. This core collection contained the 98% of the total morphological and molecular diversity existing in the complete collection. Diverse texture parameters were measured in this collection by means of a texturo-meter and several morphological descriptors related with berry and bunch dimensions were studied. VvPel was sequenced in the core collection, and 32 SNPs and 15 haplotypes were identified. LD was low enough to provide a high resolution power, and neutrality tests pointed out to the possible existence of balanced selection over the gene in the collection. Some of the polymorphisms associated significantly with texture parameters, explaining part of the variation found for the trait. These results constitute the beginning of the dissection of the complex trait berry texture in table grapes. 59 
O-40 Quantitative analysis of texture and fertility in table grape I. Carreño* 1 , J.A. Cabezas 2 , R. Arroyo-García 3 , J.M. Martínez-Zapater 4 , J. Carreño 1 , L. Ruiz- García 5 1 Dpto. Viticultura. IMIDA. C/ Mayor s/n, 30150 La Alberca, Murcia, Spain; 2 Dpto. Investigación Agroalimentaria, IMIDRA, Finca El Encín. Alcalá de Henares, Spain; 3 Dpto. Biotecnología, INIA-CBGP. Campus de Montegancedo, Pozuelo de Alarcón, Madrid, Spain; 4 Instituto de Ciencias de la Vid y del Vino (CSIC, Universidad de La Rioja, Gobierno de La Rioja), CCT, C/ Madre de Dios 51, 26006 Logroño, La Rioja, Spain; 5 Dpto. Biotecnología y Protección de Cultivos. IMIDA. C/ Mayor s/n, 30150 La Alberca, Murcia, Spain *Corresponding author: ivanncr@gmail.com Berry texture and grapevine fertility are traits of major relevance for table grape (Vitis vinifera L.) breeding. In order to develop molecular markers linked to those traits for their use in MAS (Marker Assisted Selection), we have carried out quantitative genetic analysis in seedless x seedless table grape progeny, consisting of 78 individuals from a cross between cultivars ‘Ruby Seedless’ and ‘Moscatuel’. A framework linkage map was constructed for each parent. The homologous linkage groups (LGs) were used to generate an integrated map spanning 1191 cM with 165 markers (120 SSR and 45 SNP) at an average distance between loci of 7.2 cM. Berry texture and fertility were evaluated in four growing seasons. Berry texture was determined using the TA.XT2 Texture Analyser. Fertility was quantified as number of inflorescences per shoot. QTL (Quantitative Trait Loci) analyses were carried out using a multiple QTL model (MQM) based on QTLs detected at least during two previous seasons via interval mapping. Both LG specific and genome-wide significance thresholds corresponding to α=0.20 were used as minimum values for QTL detection. QTLs for texture with LOD values higher than LG thresholds were consistently detected on LGs 9, 11 and 18, explaining between 7.5% and 24.5% of total variance. For fertility, a QTL with a LOD value higher than the genome-wide threshold was identified on LG 14, explaining up to 27.2% of total variance. 60 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
Page 7 and 8: Tuesday August 3, 2010 Oral Session
Page 9 and 10: Poster Session 2 1:30 PM-3:00 PM Po
Page 11 and 12: P-105 Pathogen responsiveness and v
Page 13 and 14: Table of Contents Keynote Presentat
Page 15 and 16: K-2 Progress in grapevine breeding
Page 17 and 18: K-4 Genetics and Genomics of Grapev
Page 19 and 20: O-2 Additional homologs of the Ren1
Page 21 and 22: O-4 Y. Xu and Y.Wang* Introduction
Page 23 and 24: O-5 Identification of an R2R3 MYB t
Page 25 and 26: O-7 Sequencing of the phylloxera re
Page 27 and 28: O-9 Dissection of defense pathways
Page 29 and 30: O-11 Recent trends and technologies
Page 31 and 32: O-13 Brazilian Grape Breeding Progr
Page 33 and 34: O-15 Development of rootstocks for
Page 35 and 36: O-17 L. Wang* 1 , S. Li 1, 2 , P. F
Page 37 and 38: O-19 Integrated analysis of transcr
Page 39 and 40: O-20 Deciphering the chromosome str
Page 41 and 42: O-22 Identification of table grapes
Page 43 and 44: O-24 Metagenomic sequencing as a to
Page 45 and 46: O-26 Regulation of bud fruitfulness
Page 47 and 48: O-28 Haplotype structure and cluste
Page 49 and 50: O-30 Annette Nassuth Function of Vi
Page 51 and 52: 10 to 25% of the chlorotic symptoms
Page 53 and 54: O-34 Cloning and characterization o
Page 55 and 56: O-36 New insight into the genetics
Page 57: O-38 Co-Localization of QTLs for Se
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
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P-34 Genome-wide identification of
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P-36 Looking for genetic polymorphi
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P-38 Characterization of a new horm
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P-40 Towards a characterization of
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the overall identified polymorphism
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P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
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P-48 ‘Cioutat’: a somatic varia
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P-50 Estimation of protein spot var
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glucosyltransferase, anthocyanidin
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P-53 Marker-free transgenic grapevi
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P-55 Agrobacterium rhizogenes-media
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P-57 Phenotypic evaluation of ‘Th
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P-59 Phenotypic divergence among gr
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P-61 Grepevine variety determinatio
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agricultural context. This network
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P-64 Genetic structure in a large r
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P-66 Analysis of grape rootstocks b
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P-68 Italian wild grapevine: a stat
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Rebula-Robola group were genotyped
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P-71 Viticultural performance of so
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P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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