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O-50 Allelic variation in the domestication gene VvmyA1 in natural grape populations (Vitis vinifera ssp sylvestris) D. Carrasco 1 , R. Fernández 1 , E. Revilla 2 , R. Arroyo-García* 1 . 1 CBGP-INIA. Dpto Biotecnología, Campus de Montegancedo, Pozuelo de Alarcón, Madrid, Spain; 2 Departamento de Química Agrícola, Universidad Autónoma de Madrid, Madrid. Spain *Corresponding author: rarroyo@inia.es Skin color is one of the most important qualities used as the basis for selection in breeding programs for wine and table grapes. The color is determinate by the quantity and composition of anthocyanins. Color-skinned cultivars accumulate anthocyanin in their skins, whereas white skin cultivars do not. Myb-related transcriptional factors genes such as VvmybA1 regulate anthocyanins biosynthesis. Wild grape (Vitis vinifera ssp sylvestris) is the ancestor of the cultivated grapevine (Vitis vinifera ssp sativa), berries are black-colored. We have analyzed the quantity and composition of anthocyanins in wild grapes and we have found typical characteristics slightly different from cultivated grapevine. This report examines the allelic variants present in over 200 wild grape accessions from both ends of the Mediterranean basin (Iberian Peninsula and Anatolian Peninsula). Results provide evidence that variation in one transcriptional regulator has generated a new allelic series that has been not found in cultivated grapevine. Furthermore, the allelic series showed correlation with anthocyanins content. These findings provide information about the evolution of grapes since domestication took place and have direct implications in wine quality of this important crop. 71 
O-51 Marker-assisted breeding: Identification of monoterpensynthases in grapevine (Vitis vinifera L.) and their potential as markers in breeding O. Bitz* 1 , M. Nitsch 1 , O. Budich 1 , M. Schmidt 1 , T. Wolf 2 , M. Hey 1 , E. Ruehl 1 1 Geisenheim Research Center, Geisenheim, Germany; 2 Boehringer Ingelheim Pharma GmbH & Co. KG, Binger Straße 173, 55216 Ingelheim, Germany *Corresponding author: bitz@fa-gm.de Monoterpenes are considered to be one of the major classes of volatile flavour components in many grapevine varieties and are typical for fruity and floral white wine. Variety character largely depends on kind and amount of monoterpenes. GC-MS analysis showed great differences among many cultivars and clones. They are significantly influenced by environment and ripening stage, which makes a systematic clone evaluation based on phenotype difficult. The objective of this study was to identify genes involved in metabolic pathways leading to characteristic monoterpenes. The biosyntheses and molecular genetics of monoterpenes have been widely studied in various species. The recently published genome of grapevine and sequences from several plant species available in databases enable us to identify and characterise potential terpensynthases (linalool-, geraniol-, α-terpineol-synthases) in grapevine. In this study several previously unknown putative terpensynthases of grapevine were identified and their potential for marker-assisted breeding assessed. A complete set of markers for relevant monoterpenes would enable marker-assisted clone selection without the influence of environment and grape ripening/ sampling stage thus increasing the effectiveness and efficiency of clone development and genotype conservation. 72 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
Page 19 and 20: O-2 Additional homologs of the Ren1
Page 21 and 22: O-4 Y. Xu and Y.Wang* Introduction
Page 23 and 24: O-5 Identification of an R2R3 MYB t
Page 25 and 26: O-7 Sequencing of the phylloxera re
Page 27 and 28: O-9 Dissection of defense pathways
Page 29 and 30: O-11 Recent trends and technologies
Page 31 and 32: O-13 Brazilian Grape Breeding Progr
Page 33 and 34: O-15 Development of rootstocks for
Page 35 and 36: O-17 L. Wang* 1 , S. Li 1, 2 , P. F
Page 37 and 38: O-19 Integrated analysis of transcr
Page 39 and 40: O-20 Deciphering the chromosome str
Page 41 and 42: O-22 Identification of table grapes
Page 43 and 44: O-24 Metagenomic sequencing as a to
Page 45 and 46: O-26 Regulation of bud fruitfulness
Page 47 and 48: O-28 Haplotype structure and cluste
Page 49 and 50: O-30 Annette Nassuth Function of Vi
Page 51 and 52: 10 to 25% of the chlorotic symptoms
Page 53 and 54: O-34 Cloning and characterization o
Page 55 and 56: O-36 New insight into the genetics
Page 57 and 58: O-38 Co-Localization of QTLs for Se
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69: O-49 Molecular survey of Georgian t
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
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P-48 ‘Cioutat’: a somatic varia
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P-50 Estimation of protein spot var
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glucosyltransferase, anthocyanidin
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P-53 Marker-free transgenic grapevi
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P-55 Agrobacterium rhizogenes-media
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P-57 Phenotypic evaluation of ‘Th
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P-59 Phenotypic divergence among gr
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P-61 Grepevine variety determinatio
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agricultural context. This network
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P-64 Genetic structure in a large r
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P-66 Analysis of grape rootstocks b
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P-68 Italian wild grapevine: a stat
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Rebula-Robola group were genotyped
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P-71 Viticultural performance of so
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P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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