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P-60 The Brazilian Grape Germplasm Bank: phenology and resistance to main fungal diseases U.A. Camargo, J.D.G. Maia, C.A.E. Machado, P.S. Ritschel* Embrapa Grape and Wine, Bento Gonçalves, Rio Grande do Sul, Brazil *Corresponding author: patricia@cnpuv.embrapa.br In recent years viticulture has reached a very important role in Brazilian fruit production, not only in temperate zones, but also as an alternative for tropical regions. These different climates require cultivars with wide-ranging production cycles. In temperate climates, such as in Southern Brazil, cultivars with different production cycles allow an increase in the harvesting period. Early grapes, demanded by growers in tropical zones such as in northeast Brazil, allow more than one annual harvest. The most important fungal diseases in Brazil are downy mildew (Plasmopara viticola), powdery mildew (Uncinula necator), anthracnose (Elsinoe ampelina), and bunch rot (Botrytis cinerea and other agents). In some cases, phytosanitary treatment can reach 30% of production costs. Genetic breeding can play a role in the development of new cultivars with different production cycles and greater tolerance to the main fungal diseases. The purpose of this work is to evaluate the phenology and disease incidence of grape accessions of the Brazilian germplasm bank in order to give support to the breeding program. For ten years, 700 accessions were evaluated and classified as very early (0.6%), early (13.9%), medium (43.6%), late (41.8%) or very late (0.3%). In the same period, 1,100 accessions were evaluated for disease resistance. The Brazilian grape germplasm bank maintains resistance sources to the main fungal grape diseases which occur in the country. However, resistance to downy mildew and anthracnose are less widespread in the studied sample. More information about the Brazilian grape germplasm bank can be found on the web at http://www.cnpuv.embrapa.br/prodserv/germoplasma /. These results assist in the development of new grape cultivars, in order to give support to the evolution and expansion of Brazilian viticulture. 139 
P-61 Grepevine variety determination from herbarium and arheological specimens N. Malenica 1 , S. Simon 2 , E. Maletic 3 , I. Pejic* 2 1 University of Zagreb, Faculty of Science, Department of Molecular Biology, Zagreb, Croatia; 2 University of Zagreb, Faculty of Agriculture, Department of Plant Breeding, Genetics and Biometrics, Zagreb, Croatia; 3 University of Zagreb, Faculty of Agriculture, Department of Viticulture and Enology, Zagreb, Croatia *Corresponding author: ipejic@agr.hr Genotyping old grapevine samples, in particular variety identification via microsatellite profiling is still far from being routine. The successful identification depends on several factors, the age of the investigated material being the most obvious one. In addition, the amount and integrity of DNA depends on conditions of samples storing. Contamination and fragmentation processes make the isolated ancient DNA (aDNA) a rather difficult template for PCR amplification. Three old grapevine samples were investigated in this study. Two samples were taken from approximately 90-years old herbarium of cv. Tribidrag, a Croatian autochthonous grapevine variety not anymore present in the germplasm collections and one was approximately 2000 years old underwater archeological grapevine specimen (woody parts). Several different approaches of DNA isolation were tested. However, the use of a commercial plant DNA isolation kit showed to be the best choice in terms of yield and prevention of possible contamination with temporary grapevine DNA. The initial attempts to amplify standard grapevine microsatellite loci by standard PCR protocol failed, most likely due to low copy number of template DNA. Therefore, the whole genome amplification (WGA) kit was successfully applied to overcome this problem. The WGA approach worked in case of one herbarium sample and the archeological specimen. DNA fragments ranging between 100 and 500 bp were cloned and sequenced. While the isolated DNA from the underwater archeological sample was proved to be from different origins (not from grapevine), indicating contamination of the specimen, the six sequenced clones from the herbarium specimen corresponded to six different grapevine chromosomes. Therefore, we used the WGA-processed herbarium sample and managed to PCR-amplify the VVS2 locus, thereby demonstrating the proof of principle. Eventually, we successfully genotyped the same template using six standard microsatellite loci (VVS2, VVMD5, VVMD7, VVMD27, VvZAG62 and VvZAG79). The obtained SSR profile was identical to Zinfandel/Primitivo/Crljenak Kastelanski, except for the VVS2 locus which was homozygous (141/141) instead of heterozygous (131/141). In the light of these results we hypothesize on the origin of Zinfandel as an old Croatian variety under the historical name Tribidrag, which was being used in documents dating as late as 15 th century. 140 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
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P-8 Monoterpene levels in different
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P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137: P-59 Phenotypic divergence among gr
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175 and 176: P-95 Reaction of grape rootstocks t
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179 and 180: P-99 Berry cracking caused powdery
Page 181 and 182: P-101 Elicitation of grapevine defe
Page 183 and 184: P-103 The biological characteristic
Page 185 and 186: P-105 Pathogen responsiveness and v
Page 187 and 188: P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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