conference schedule and program with abstracts - Horticulture ...
conference schedule and program with abstracts - Horticulture ...
conference schedule and program with abstracts - Horticulture ...
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P-107<br />
Screening grape hybrid families <strong>with</strong> molecular markers linked to resistance genes<br />
D. Katula-Debreceni 1 , A. Veres 1 , A.K. Lencsés 1 , A. Szőke 1 , P. Kozma 2 , L. Kovács 3 , E. Kiss* 1<br />
1 Szent István University, Institute of Genetics <strong>and</strong> Biotechnology, Páter K. u. 1. H-2100<br />
Gödöllő, Hungary; 2 Research Institute of Viticulture <strong>and</strong> Enology, Pázmány P. u. 4. H-7634<br />
Pécs, Hungary; 3 Missouri State University, Springfield, Missouri, USA<br />
*Corresponding author: kiss.erzsebet@mkk.szie.hu<br />
Gene pyramiding is a method to breed grape varieties for durable disease resistance. The use of<br />
resistance gene-linked DNA markers reduces the volume of breeding experiments, since it<br />
facilitates the identification of progeny seedlings that have inherited the desired gene shortly<br />
after germination <strong>and</strong> allows the reduction of population size. Saturating the grape genome <strong>with</strong><br />
molecular markers, the construction of genetic linkage maps, <strong>and</strong> the recent publication of the<br />
Vitis vinifera genome sequence enable breeders to select the desired genotype directly. To<br />
combine powdery <strong>and</strong> downy mildew resistance genes, Kozma et al. (Research Institute of<br />
Viticulture <strong>and</strong> Enology, Pécs) produced the following hybrid families: BC 4 (VRH 3082-1-42) x<br />
’Kishmish vatkana’, BC 4 x ’Kishmish moldavskij’, ’Génuai zamatos’ x ’Kishmish vatkana’,<br />
(’Laszta’ x. ’Dzh<strong>and</strong>zhal kara’) x (’Katta kurgán x Perlette’), where BC 4 derives from a V.<br />
vinifera x Muscadinia rotundifolia cross. Our aim was to select the individuals containing the<br />
powdery (PM) <strong>and</strong>/or downy mildew (DM) major resistance genes of different origin<br />
(Muscadinia rotundifolia-Run1, Rpv1, V. vinifera-Ren1, <strong>and</strong> PM <strong>and</strong> DM QTLs of ’Seyve-<br />
Villard’) <strong>with</strong> SSR, CB <strong>and</strong> SCAR markers. PCR products were separated on 8% polyacrilamide<br />
(ALFExpress) <strong>and</strong> 4% Metaphor gel. Our data corroborated earlier findings that the M.<br />
rotundifolia-derived Rpv1 <strong>and</strong> Run1 loci are closely linked. We compared the symptomless<br />
progenies derived from the cross BC 4 x ’Kishmish vatkana’ <strong>and</strong> (’Laszt’ x ’Dzs<strong>and</strong>zsal kara’) x<br />
(’Katta kurgán’ x ’Perlette’) using resistance linked markers. We observed that alleles of SSR<br />
markers linked to the PM resistance gene Ren1 in the linkage group 13 are the same, suggesting<br />
that PM resistance locus of ’Kishmish vatkana’ <strong>and</strong> ’Dzh<strong>and</strong>zhal kara’ are presumably identical.<br />
Studies of QTL markers are in progress.<br />
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