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P-41 Assessing the genetic variability of grape clones S. Vezzulli* 1 , L. Leonardelli 1 , U. Malossini 2 , M. Stefanini 1 , R. Velasco 1 , C. Moser 1 1 IASMA Research and Innovation Centre, Fondazione Edmund Mach, Genomics and Crop Biology Area, San Michele a/Adige, Trento, Italy; 2 IASMA Centre for Technology Transfer, Fondazione Edmund Mach, San Michele a/Adige, Trento, Italy *Corresponding author: silvia.vezzulli@iasma.it Grapevine (Vitis vinifera L.) is a long-living and woody plant grown worldwide. Its perennial nature and vegetative propagation over long periods favours accumulation and fixing of mutations within individual genotypes, which might exhibit altered phenotypes. Clonal selection, as a procedure of crop improvement, takes advantage of the identification of sports with agronomically and enologically important traits, which are vegetatively propagated to raise new grape clones. Given the high economical value, as reflected by related patenting and legal issues, their identification is of great relevance. While cultivar identification in grapevines is traditionally based on ampelographic descriptors and on reference microsatellite (SSR) profiles, clone discrimination is not possible with such tools. To allow true genetic identification of clones, new specific molecular markers have to be implemented. Given the availability of the Pinot Noir (clone ENTAV 115) genome sequence, it is timely to use techniques based on the polymorphism information of specific genomic sequences of interest, such as DNA transposons and retrotransposons. Transposable elements (TEs), which possess the capability to change their genomic location, are indeed potential source of mutations leading to clonal variation. An alternative way to assess clonal genetic diversity consists of the study of various polymorphisms at different layers; clonal mutations can occur either at both L1 and L2 layers or at a single layer. In this work, to fathom clonal genetic variability within six wine grape cultivars, we adopted three different methods. Firstly, we have carried out a Transposon Display analysis on 141 grape genotypes, which correspond to 47 clones (both registered and biotypes) belonging to the Pinot Noir, Pinot Gris, Pinot Blanc, Meunier, Teroldego and Gewürztraminer cultivars in 3 biological replicates (plants). We have tested them using 17 primers targeting specific regions (LTRs, LTR downstream or upstream, ORF) of 6 different TE families. Secondly, by using 6 triallelic SSR markers we have analysed four tissues (leaf, berry skin, flesh and root) of the 19 registered clones. Finally, we have genotyped the same set of samples by SNPlex TM Genotyping System, testing about 500 electronic SNPs identified in coding and non-coding regions of the mentioned grape genome. Based on SNPlex results, we are currently resequencing the regions with a high level of polymorphism to confirm the presence of clonal mutations. Here we report the results of 117 
the overall identified polymorphisms enabling molecular characterization of clones within international and local grape varieties. 118 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115: P-40 Towards a characterization of
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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