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O-27 Transcriptional analysis of inflorescence and tendril development J. Díaz-Riquelme* 1,2 , J. Grimplet 1 , M.J. Carmona 2 , J.M. Martínez-Zapater 1 1 Instituto de Ciencias de la Vid y del Vino (CSIC, UR, Gobierno de La Rioja), CCT, Logroño, Spain; 2 Departamento de Biotecnología, ETSIA, Universidad Politécnica de Madrid, Ciudad Universitaria, Madrid, Spain *Corresponding author: jdiaz@cnb.csic.es Tendrils and inflorescences are organs with very different biological function (climbing or reproduction) but that share a common ontogenetic origin in the Vitaceae. In fact, they can substitute for each other depending on hormonal treatments or environmental conditions, and it is common to observe intermediate structures in the vineyards. Reproductive development is strongly regulated at the transcriptional level in plants. In order to characterize the regulation of inflorescence and tendril development we have performed a transcriptional analysis along the development of these two organs, using the Grapegen Genechip TM . This Affymetrix GeneChip TM contains 23000 probe sets representing approximately 50% of the annotated genes in the grapevine genome. In this experiment we analyzed inflorescences at six different developmental stages, from inflorescences dissected from stage B1 latent buds, without flower meristems, to pre-anthesis inflorescences (bearing fully developed flowers). In addition, tendrils were analyzed at three time points, from the first emerged tendril in the apex to what was considered as a mature tendril. The results of principal components analysis of the inflorescence and tendril time-course expression data showed that the first four principal components explain 82% of the total variation. Tendril expression variation was basically modelled by PC1 (41% of total variation), while more principal components were needed to explain inflorescence expression variation due to the complexity of the structures. The analysis of the inflorescence series allowed us to observe a main inflection point at the pre-anthesis stage, while the two first stages and the two intermediate stages seemed to be more homogeneous respectively. This inflection point is mainly due to the expression of a group of genes involved in chromatin packaging and remodelling, cell cycle control, and gibberellin metabolism and response. Functional analysis of differentially expressed genes between tendrils and inflorescences revealed that transcription factors are the most over-represented functional group among inflorescence specific genes, while tendril specific genes mainly encode auxin related transcription factors, cytokinin metabolism related genes and proteins with function in photosynthesis. 47 
O-28 Haplotype structure and cluster phenotype associations for the VvTFL1A gene L. Fernandez* 1 , L. Le Cunff 2 , A. Fournier-Level 3 , T. Lacombe 3 , J.M. Boursiquot 3 , P. This 3 , J-M. Martinez Zapater 1 1 Instituto de Ciencias de la Vid y del Vino (ICVV), (CSIC, Universidad de La Rioja, Gobierno de La Rioja), CCT C/Madre de Dios 51, 26006 Logroño, Spain; 2 UMT geno-vigne® (IFV- INRA-SupAgro) Montpellier Cedex, France; 3 INRA-SupAgro, UMR DIA-PC, Campus SupAgro/INRA, Montpellier Cedex, France *Corresponding author: lucie.fernandez@icvv.es VvTFL1A overexpression due to transposon insertion in its promoter region causes delayed flowering and production of a ramose cluster in the ‘Carignan’ RRM somatic variant (Fernandez et al. 2010). In order to analyze the possible contribution of this gene to cluster phenotypic variation in cultivated grapevine (Vitis vinifera L. subsp. sativa), we have characterized its nucleotide diversity and performed association analyses among detected sequence polymorphisms and VvTFL1A expression and cluster traits. We sequenced a total of 3489 bp of the VvTFL1A gene (corresponding to 2177 bp of promoter and 1312 bp of coding sequence) in a core collection designed to maximize phenotypic variation at 50 agromically relevant traits (Barnaud et al. 2006). Nucleotide polymorphisms were highly abundant in the promoter and intron sequences of the gene and some of them revealed significant associations (P
Page 1 and 2: Date Time Session Topic Sunday Augu
Page 3 and 4: Monday August 2, 2010 Opening Plena
Page 5 and 6: Topic: Adaptation to soils and clim
Page 7 and 8: Tuesday August 3, 2010 Oral Session
Page 9 and 10: Poster Session 2 1:30 PM-3:00 PM Po
Page 11 and 12: P-105 Pathogen responsiveness and v
Page 13 and 14: Table of Contents Keynote Presentat
Page 15 and 16: K-2 Progress in grapevine breeding
Page 17 and 18: K-4 Genetics and Genomics of Grapev
Page 19 and 20: O-2 Additional homologs of the Ren1
Page 21 and 22: O-4 Y. Xu and Y.Wang* Introduction
Page 23 and 24: O-5 Identification of an R2R3 MYB t
Page 25 and 26: O-7 Sequencing of the phylloxera re
Page 27 and 28: O-9 Dissection of defense pathways
Page 29 and 30: O-11 Recent trends and technologies
Page 31 and 32: O-13 Brazilian Grape Breeding Progr
Page 33 and 34: O-15 Development of rootstocks for
Page 35 and 36: O-17 L. Wang* 1 , S. Li 1, 2 , P. F
Page 37 and 38: O-19 Integrated analysis of transcr
Page 39 and 40: O-20 Deciphering the chromosome str
Page 41 and 42: O-22 Identification of table grapes
Page 43 and 44: O-24 Metagenomic sequencing as a to
Page 45: O-26 Regulation of bud fruitfulness
Page 49 and 50: O-30 Annette Nassuth Function of Vi
Page 51 and 52: 10 to 25% of the chlorotic symptoms
Page 53 and 54: O-34 Cloning and characterization o
Page 55 and 56: O-36 New insight into the genetics
Page 57 and 58: O-38 Co-Localization of QTLs for Se
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
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P-22 Expression of early light-indu
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P-24 The development of molecular m
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P-26 Identification of ABA inducibl
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P-28 In vitro selection of HYP tole
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P-30 In silico SNP detection for ge
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P-32 Vitis vinifera genome annotati
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P-34 Genome-wide identification of
Page 111 and 112:
P-36 Looking for genetic polymorphi
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P-38 Characterization of a new horm
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P-40 Towards a characterization of
Page 117 and 118:
the overall identified polymorphism
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P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
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P-48 ‘Cioutat’: a somatic varia
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P-50 Estimation of protein spot var
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glucosyltransferase, anthocyanidin
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P-53 Marker-free transgenic grapevi
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P-55 Agrobacterium rhizogenes-media
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P-57 Phenotypic evaluation of ‘Th
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P-59 Phenotypic divergence among gr
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P-61 Grepevine variety determinatio
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agricultural context. This network
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P-64 Genetic structure in a large r
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P-66 Analysis of grape rootstocks b
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P-68 Italian wild grapevine: a stat
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Rebula-Robola group were genotyped
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P-71 Viticultural performance of so
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P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
Page 187 and 188:
P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
Page 191 and 192:
P-112 Evaluation of grapevine germp
Page 193 and 194:
P-114 Evaluation of four new rootst
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