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P-100 Gray mold (Botrytis cinerea) induced berry cracking in ‘Fujiminori’ table grapes (Vitis sp.) D. Kim*, S.-I. Oh, I.-C. Son Dept. of Horticultural Science, Chungbuk National University, Cheongju, Korea *Corresponding author: dkpomo@chungbuk.ac.kr Tetraploid grape cultivars including ‘Fujiminori’ are cultivated for higher quality and marketability in table grape production using protected rain-shelter cultivation system in Korea. Gray mold (Botrytis cinerea) is one of the most serious diseases for tetraploid grape cultivation. To find out the berry cracking symptoms caused by B. cinerea infection, morphological and cellular characteristics were observed using cracking susceptible ‘Fujiminori’ grapevines. The intact berry rate indicative of marketability was decreased by 62.7% after the inoculation compared to 89.3% in the control vines. Cluster weight and numbers of berries per cluster in inoculated vines was also significantly decreased as 535.6 g and 28.2 compared with 842.2 g and 40.2 in non-inoculated controls. The decreasing of berry firmness from 1.31 to 1.08 kg/Ø5mm in gray mold inoculated vines induced a three-fold higher berry cracking rate under critical turgor pressure from 18.0 to 52.0%. The cell wall content per 10 g of pericarp, which can be an indicator for strength of cell wall structure, was reduced 45.9% from 453.7 to 245.3 mg in gray mold infected ‘Fujiminori’ vines. The increase in polygalacturonase activity from 0.143 to 0.204 units/mL after the infection of B. cinerea could also accelerate the cell wall degradation and berry cracking. Though there are no visible disease symptoms on the berry surface in early growth stages before veraison, invaded hypha caused by B. cinerea infection are already promoting cell breakdown between the sub-epidermal and the epidermal layer by secreted cell wall degradation enzymes. Therefore, the susceptibility and the infection of B. cinerea before veraison can be an important trigger of berry cracking. 181 
P-101 Elicitation of grapevine defense responses against downy mildew caused by Plasmopara viticola M. Selim 1, 2* , G. Langen 2 , B. Berkelmann-Löhnertz 3 , K-H. Kogel 2 , D. Evers 1 1 Centre de Recherche Public – Gabriel Lippmann, Department Environment and Agro- Biotechnologies (EVA), Belvaux, Luxembourg; 2 Research Centre for BioSystems, Land Use and Nutrition, Justus Liebig University Giessen, Germany; 3 Geisenheim Research Center, Institute of Biology, Department of Phytomedicine, Geisenheim, Germany *Corresponding author: selim@lippmann.lu Plasmopara viticola, the casual agent of downy mildew, is one of the most destructive grapevine diseases in Europe and in the eastern half of the United States. In recent decades, induced resistance to P. viticola has gained increasing attention as a strategy to control the disease in a more sustainable way. This work aims at triggering the plant’s own defence mechanism/s against P. viticola by some selected elicitors, through prophylactic as well as curative strategies. To evaluate the level of induced resistance, an integrated approach will be followed to assess the efficacy of the elicitors used as well as to understand their mode of action. This includes synthesis of phytoalexins as well as PR-proteins, cell death (hypersensitive response) and defense gene and protein expression, to name a few. Disease severity as well as disease development have been assessed. Callose formation was measured as an indicator of the activation of a defence reaction. First experiments indicated different infection intensities as a result of different elicitor applications, which in turn indicateed that some elicitors had the effect of reducing the spread of the disease. For disease development, haustoria formation and hypersensitive response were measured using epifluorescence microscopy. At the molecular level, the gene expression of some candidate genes was measured using real-time PCR. The initial results showed differential expression of these genes between the different treatments. 182 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
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P-8 Monoterpene levels in different
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P-10 Usefulness of the SCC8 scar ma
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P-12 The onset of berry ripening is
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P-14 Morphological characteristics
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P-16 A genetic analysis of berry qu
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P-18 Use of molecular markers for s
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P-20 Characterization of key compon
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P-22 Expression of early light-indu
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P-24 The development of molecular m
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P-26 Identification of ABA inducibl
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P-28 In vitro selection of HYP tole
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P-30 In silico SNP detection for ge
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P-32 Vitis vinifera genome annotati
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P-34 Genome-wide identification of
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P-36 Looking for genetic polymorphi
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P-38 Characterization of a new horm
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P-40 Towards a characterization of
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the overall identified polymorphism
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P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
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P-48 ‘Cioutat’: a somatic varia
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P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175 and 176: P-95 Reaction of grape rootstocks t
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179: P-99 Berry cracking caused powdery
Page 183 and 184: P-103 The biological characteristic
Page 185 and 186: P-105 Pathogen responsiveness and v
Page 187 and 188: P-107 Screening grape hybrid famili
Page 189 and 190: P-109 Characterization of an ankyri
Page 191 and 192: P-112 Evaluation of grapevine germp
Page 193 and 194: P-114 Evaluation of four new rootst
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