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P-102<br />

Optimizing the breeding of Pierce’s disease resistant wine grapes <strong>with</strong><br />

marker-assisted selection<br />

M.A. Walker*, S. Riaz, A. Tenscher<br />

Department of Viticulture & Enology, University of California, Davis, California , USA<br />

Corresponding author: awalker@ucdavis.edu<br />

Pierce’s disease (PD) greatly limits the production of Vitis vinifera based grapevines across the<br />

southern United States. The disease is caused by a xylem-limited bacterium, Xylella fastidiosa,<br />

<strong>and</strong> is spread by the sharpshooter family of leafhoppers (Cicadellide). Many Vitis species in the<br />

southern United States are resistant to X. fastidiosa, however their resistance is multi-genic <strong>and</strong><br />

complex, which has limited breeding progress. However, resistance from several forms of V.<br />

arizonica (b43-17 <strong>and</strong> b40-14) from northern Mexico is inherited as a single dominant gene; all<br />

F1 generation offspring are resistant <strong>and</strong> F2 populations segregate 1:1 resistant:susceptible. This<br />

resistance has been genetically mapped to chromosome 14 where the locus, PdR1, is flanked by<br />

two closely linked SSR markers. Physical mapping identified a 201Kb region that encompasses<br />

PdR1, which is currently under study. The tightly linked flanking markers have been used for<br />

marker-assisted selection (MAS) for PdR1. MAS in conjunction <strong>with</strong> aggressive training in the<br />

field has brought seed-to-seed cycle down to two years. In 2010, a total of 4,360 seedlings<br />

from 34 crosses of modified BC4 seedling populations (97% V. vinifera) were marker tested <strong>and</strong><br />

over 2,000 resistant seedlings were planted in the field. These are destined for fruit <strong>and</strong> wine<br />

evaluations in 2012 prior to commercialization tests. Small-scale wines have been made from<br />

BC3 94% V. vinifera PD resistant selections <strong>with</strong> very promising results. PD resistant selections<br />

from the BC1, BC2 <strong>and</strong> BC3 level have also been planted in a severely infected area in Napa<br />

Valley. These plants are h<strong>and</strong> inoculated each year <strong>and</strong> their symptoms <strong>and</strong> bacterial titers are<br />

evaluated <strong>and</strong> compared to known resistant <strong>and</strong> susceptible controls.<br />

183


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