conference schedule and program with abstracts - Horticulture ...

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P-29 NCED expression and ABA concentrations vary with genotype in response to water deficit. R. Ghan, D. Hopper*, G.R. Cramer Department of Biochemistry and Molecular Biology University of Nevada, Reno, NV 89557 USA *Corresponding author: hopperd2@gmai.com Grape production is one of the most important agricultural commodities in the USA and is threatened by global warming. This is particularly relevant in a semi-arid state such as Nevada with a rapidly growing population competing for scarce water resources. Abscisic acid (ABA), a plant stress hormone, regulates water use efficiency and drought resistance in plants. 9-cisepoxycarotenoid dioxygenase (NCED) is the rate-limiting step in ABA biosynthesis, through the cleavage of a C 40 precursor into C 15 and C 25 products. NCED expression and ABA concentrations were determined over the course of the day. A controlled water deficit assay was developed. Preliminary results indicated varying levels of NCED transcript abundance in response to controlled water deficit in different genotypes. A large screen of 200 different genotypes is in progress. Our hypothesis is that the response of NCED to water deficit will be correlated with water use efficiency and drought resistance; that is, a larger response of NCED in different genotypes will be associated with greater drought resistance and water use efficiency. Experiments are in progress to test this hypothesis. A summary of these results will be presented. 105 
P-30 In silico SNP detection for genes of the anthocyanin metabolism in Vitis G. Dequigiovanni, P.S. Ritschel, V. Quecini* Embrapa Uva e Vinho, Bento Gonçalves, Rio Grande do Sul, Brazil *Corresponding author: vera@cnpuv.embrapa.br Grape flavonoids, especially anthocyanins, are important contributors to color, taste, antioxidant and nutraceutic properties (such as protection against cardiovascular diseases and cancer) in fresh fruit and processed products. Breeding programs and wild Vitis germplasm banks contain wide genetic variation in anthocyanin biosynthesis- and metabolism-associated traits, indicating the complex genetic control of the process throughout plant development. Expressed sequence tag (EST) and genomic databases were investigated using bioinformatic tools to identify SNP markers present in structural and regulatory genes associated with anthocyanin metabolism in Vitis. We have identified more than 1,000 putative SNPs in nine structural and twelve regulatory genes of anthocyanin metabolism in Vitis. The data suggest that the identified genetic variation is sufficient to distinguish among V. vinifera cultivars and hybrids and wild species. Regulatory genes were shown to be more rapidly evolving than structural enzyme-coding sequences. The evolutionary rate of the sequences associated with regulatory factors also appears to be differential, with higher levels of conservation found in the MYB family of transcriptional regulators associated to anthocyanin regulation. After experimental validation, the predicted polymorphisms will provide tools for further mapping, genome structure, and functional studies. 106 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
Page 53 and 54: O-34 Cloning and characterization o
Page 55 and 56: O-36 New insight into the genetics
Page 57 and 58: O-38 Co-Localization of QTLs for Se
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103: P-28 In vitro selection of HYP tole
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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