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P-70 Proteomic analysis among different ‘Aglianico’ ecotypes A.S. Negri*, B. Prinsi, S. Imazio, G. de Lorenzis, O. Failla, A. Scienza, M. Cocucci, L. Espen Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Milano, Italy *Corresponding author: alfredo.negri@unimi.it Molecular markers are extensively used for the identification of different vines. Among them, one of the techniques of major success is based on the analysis of the Simple Sequence Repeats (SSRs). Although they are useful in discriminating among different varieties, it is unusual that they can distinguish clones deriving from the same vine and there is a strong request for devices that could achieve this purpose. In this view, it could be interesting to evaluate the potential of proteomics. Using this approach, for instance, it was possible to define the biodiversity of six ecotypes of Arabidopsis thaliana. Moving from these considerations, three ecotypes of cv. Aglianico (Taurasi, Taburno and Vulture) were compared. They look identical but give rise to wines that are characterized by different qualitative features. Through the use of 20 SSRs markers, it was demonstrated that they belong to the same cultivar because it was not possible to find any differences. The proteomic analysis was conducted comparing the 2-DE patterns of berry exocarp in order to set up a method useful in searching for distinctive traits. On the basis of previous studies that underlined the changes of the proteome of this tissue during maturation or driven by the environment, the analysis was performed considering two moments of maturation during the years 2008 and 2009. In order to deal with the high number of data and to sort the variability among them, gels analysis, after their alignment, was carried out through multivariate statistical techniques such as Principal Component Analysis and Linear Discriminant Analysis. The application of such approaches allowed to distinguish the three ecotypes and to isolate the proteins more responsible of the discrimination models, then characterized by mass spectrometry. These results confirm the effectiveness of this approach integrating proteomics and multivariate statistics for the study of intravarietal diversity. 151 
P-71 Viticultural performance of some ‘Cabernet Sauvignon’ clones M. Gatti 1 , S. Civardi 1 , F. Ferrari 2 , N. Fernandes 1 , L. Bavaresco* 1 1 Istituto di Frutti-Viticoltura, Piacenza, Italy; 2 Istituto di Chimica Agraria e Ambientale, Università Cattolica del S. Cuore, Piacenza, Italy *Corresponding author: luigi.bavaresco@unicatt.it Ten clones of V. vinifera L. ‘Cabernet Sauvignon’ from different origins (Italy, clone R5; Chile, clone ISV117; California, clones VCR8 and ISV2; France, clones 191, 341, 338, 169, 685; Argentina, clone ISV105), were grafted on V. Berlandieri x V. riparia S.O.4 rootstock and grown in pots to compare vegetative (phenological phases; shoot growth; internode length), productive (bud fertility, grape yield, mean cluster and berry weight) and qualitative (soluble solids, pH, titratable acidity, anthocyanins, polyphenols, trans-resveratrol) parameters. Meteorological conditions were recorded as well. According to analysis of variance and discriminant analysis the clones have been grouped in four clusters irrespective of their origin. 152 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
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P-8 Monoterpene levels in different
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P-10 Usefulness of the SCC8 scar ma
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P-12 The onset of berry ripening is
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P-14 Morphological characteristics
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P-16 A genetic analysis of berry qu
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P-18 Use of molecular markers for s
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P-20 Characterization of key compon
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P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149: Rebula-Robola group were genotyped
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175 and 176: P-95 Reaction of grape rootstocks t
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179 and 180: P-99 Berry cracking caused powdery
Page 181 and 182: P-101 Elicitation of grapevine defe
Page 183 and 184: P-103 The biological characteristic
Page 185 and 186: P-105 Pathogen responsiveness and v
Page 187 and 188: P-107 Screening grape hybrid famili
Page 189 and 190: P-109 Characterization of an ankyri
Page 191 and 192: P-112 Evaluation of grapevine germp
Page 193 and 194: P-114 Evaluation of four new rootst
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