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P-58 Characterization of grape cultivars through Expressed Sequence Tag Polymorphism (ESTP) M.F. Moura* 1 , H.E. Sawazaki 1 , A.R. Verdi 2 , C.L. Messias 3 1 Instituto Agronômico - IAC. Av. Barão de Itapura, Campinas, São Paulo, Brazil; 2 Instituto de Economia Agrícola - IEA, São Paulo, São Paulo, Brazil; 3 College of Agriculture Engineering, Feagri, UNICAMP, Campinas, São Paulo, Brazil *Corresponding author: mouram@iac.sp.gov.br The Brazilian cultivars ‘Maximo IAC 138’ (a hybrid of ‘Shiraz’ and ‘Seibel’ developed by Dr. Santos Neto in the Instituto Agronômico de Campinas/IAC, SP, in 1946), ‘Rainha’ and ‘Madalena’ were characterized through Expressed Sequence Tag Polymorphism (ESTP) and restriction sites. The foliar DNA was extracted using CTAB. The PCR was done using first with eleven and then thirteen primers to find EST-SNPs (single nucleotide polymorphism) in the grapevine genome. The amplicon sequencing was done after EXO-SAP purification, using the Big Dye v. 3.0/ ABI PRISM 377 from Applied Biosystems, with three replicates. The sequences analyzed with Sequencher 3.1 (Gene Codes Corporation) were compared using the Bioedit v.7.05 and analyzed in silico for specific restriction sites (http://www.restrictionmapper.org/). It was observed that one primer named 28 differentiated ‘Rainha’ from ‘Máximo’ in three regions, while another primer, 126, differentiated ‘Madalena’ from ‘Rainha’ and ‘Maximo’ in two regions and ‘Maximo’ from ‘Madalena’ in a different site. The primer 136 differentiated ‘Madalena’ from cultivars ‘Maximo’ and ‘Rainha’. The other primers seem not to have amplified sequences with specific restriction site differences among cultivars. Through analysis “in silico” we observed that with primer 136, the SduI enzyme differentiated ‘Rainha’ and ‘Maximo’ from ‘Madalena’ whereas with primer 28, the IMx enzyme differentiated ‘Maximo’ from ‘Rainha’. The analyses carried out using the studied primers allowed the detection of sequences with specific restriction sites and consequently, the differentiation of’ Maximo’, ‘Rainha’ and ‘Madalena’ cultivars. 137 
P-59 Phenotypic divergence among grapevine accessions of the germplasm collection at IAC (Brazil) M.F. Moura* 1 , M.A. Tecchio 1 , A.F. Chiorato 1 , M.M. Terra 1 , E.J.P. Pires 1 , J.L. Hernandes 1 , N.F. Moura 2 , A.R. Verdi 3 1 Instituto Agronômico - IAC. Campinas, São Paulo, Brazil; 2 Escola de Agronomia e Engenharia de Alimentos/ Universidade Federal de Goiás – UFG. Rodovia Goiânia/Nova Veneza, Goiânia, Goiás, Brazil; 3 Instituto de Economia Agrícola - IEA, Av. Miguel Stefano, 3900, Água Funda, CEP: 04301-903, São Paulo, São Paulo, Brazil *Corresponding author: mouram@iac.sp.gov.br The recombination among divergent parents may result in favorable genic combinations allowing a better use of the additive, epistatic and pleiotropic effects, giving room for important characteristics such as productivity, quality and resistance to certain pathogens. Germplasm bank characterization as well as adequate statistical methods allow the identification of divergent parents. Therefore, this study aimed to evaluate the genetic divergence among 56 accessions of grapevine in the Germplasm Collection at Instituto Agronômico - IAC, characterized by eight quantitative traits. Multivariate analyses were used to quantify the divergence among the accessions. Euclidian distance, principal components and Tocher’s clustering method were applied. The Tocher’s method applied to the matrix of Euclidian distance with scores of principal components discriminated seven similar groups, with the first two comprised 69.64% and 17.86% respectively of total access. About 86.94% of the total variation was explained by the three first main components obtained with eight components analysis and 77.27% was explained by the first two main components. Thus, the multivariate analysis to study genetic diversity applied to the quantitative characters (Tocher’s clustering method and principal components) agreed among themselves, being efficient for analysis of the genetic diversity of germplasm of grapevine at IAC, which can be used for guidance in future crosses for breeding of this species. However, additional characteristics related to productivity, cycle and quality of most will be realized and added to these works of diversity. 138 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
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O-47 Intravarietal variability of C
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O-49 Molecular survey of Georgian t
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O-51 Marker-assisted breeding: Iden
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O-53 An Integrated approach to stud
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O-55 Characteristics of promising m
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P-2 PMEI and SPY: Two genes with po
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P-4 Gene-assisted selection for tab
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P-6 Polyphenolic potential of the r
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P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135: P-57 Phenotypic evaluation of ‘Th
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
Page 169 and 170: P-89 The usefulness of allotetraplo
Page 171 and 172: P-91 Drying rate of fresh berries f
Page 173 and 174: P-93 Development of table and raisi
Page 175 and 176: P-95 Reaction of grape rootstocks t
Page 177 and 178: P-97 Genetic analysis of the resist
Page 179 and 180: P-99 Berry cracking caused powdery
Page 181 and 182: P-101 Elicitation of grapevine defe
Page 183 and 184: P-103 The biological characteristic
Page 185 and 186: P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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