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P-21<br />

Anthocyanin Profiling in the Berry Skins of Five Vitis amurensis Grapes <strong>and</strong> One Related<br />

Hybrid Cultivar<br />

F. He* <strong>and</strong> J. Wang<br />

Centre for Viticulture <strong>and</strong> Enology, College of Food Science & Nutritional Engineering, China<br />

Agricultural University, Beijing 100083, P.R. China.<br />

*Corresponding author: wheyfey@yahoo.com.cn<br />

V. amurensis is the world’s most northerly grapevine species that originates in the exceptionally<br />

cold climate in northeast China, <strong>and</strong> many V. amurensis grapes have been widely used because<br />

of not only their strong cold tolerance <strong>and</strong> disease hardness, but also high pigment content.<br />

However, until now it remains no detailed description of the anthocyanin composition <strong>and</strong><br />

content in V. amurensis grapes. In this study, anthocyanin profiles in the berry skins of five V.<br />

amurensis grapes <strong>and</strong> one hybrid cultivar were analyzed by high performance liquid<br />

chromatography-electronic spray ionization-t<strong>and</strong>em mass (HPLC-ESI-MS/MS). The result<br />

showed that both the V. amurensis grapes <strong>and</strong> the hybrid cultivar contained 3,5-diglucosides of<br />

anthocyanidins <strong>with</strong> high amount in addition to 3-monoglucosides of delphinidin, cyanidin,<br />

petunidin, peonidin <strong>and</strong> malvidin that are generally detected in V. vinifera grapes. Furthermore,<br />

pelargonidin-3, 5-diglucoside <strong>and</strong> B-type vitisins of some anthocyanidin-3-glucosides were also<br />

identified in trace amount <strong>and</strong> no acetylated anthocyanins were detected in these samples. This<br />

finding will be important to utilize this germplasm resource in grapevine breeding <strong>program</strong>s <strong>and</strong><br />

to further uncover biosynthesis <strong>and</strong> regulation of anthocyanins in V. amurensis grapes.<br />

97


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