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O-41 Gene silencing as strategy to induce grapevine fan leaf virus (GFLV) resistance in grapevine rootstocks E. Torres, A. Cadavid, C. Santibáñez, F. Godoy, C. Medina, P. Arce-Johnson* P. Universidad Catholica de Chile, Fac. de Ciencias Biologicas, Santiago, Chile *Corresponding author: parce@bio.puc.cl Viral infections in grapevines cause physiological disorders that lead to foliar deformations, alterations in the berry color and finally reductions in productivity. Most viral infections in grapevine are disseminated by biological vectors and then by the vegetative propagation of infected material. More than ten viruses commonly infect the grapevine, and it is not rare to find two or three different viruses in one infected plant. There are not efficient chemical treatments against virus infections. A molecular strategy to induce virus resistance in plants is gene silencing. This strategy requires the transformation of plants with a short sequence of the pathogen in a way that a double-strand RNA structure is formed during transcription, initiating gene silencing in the host. The objective of this work is the induction of virus silencing in grapevine rootstocks plants in order to use it for grafting. It is hoped that the mobile silencing signal induces virus silencing in the scion. We have transformed rootstocks plants (110 Richter and Harmony) by co-culture of embryogenic and organogenic tissues with Agrobacterium tumefaciens carrying a silencing vector containing a sequence of the coat protein of grapevine fanleaf virus (GFLV). Twenty-three transgenic plants of the 110 Richter rootstock have been recovered, analyzed by PCR against the GFLV sequence, and propagated to obtain several plants of each line. The transgenic rootstocks have been grafted with GFLV infected plants that were positive for virus presence by RT-PCR analysis. Once the grafts were set, the RT-PCR analysis was repeated in the scion. After one month the detection of the virus has been abolished in the scion, in four of ten analyzed rootstocks lines and the same result was maintained after six months. 61 
O-42 Transcriptional changes in response to heterologous expression of a pear PGIP in grapevine A.M. Ibáñez* 1 , Y. Gogorcena 2 , C.B. Agüero 1 , R.L. Reagan 1 , S.L. Uratsu 1 , A.M. Dandekar 1 1 Department of Plant Sciences, University of California, Davis, Davis California, USA; 2 Department of Pomology, EEAD-CSIC, Zaragoza, Spain *Corresponding author: amibanez@ucdavis.edu To suppress the virulence of Xylella fastidiosa (X. fastidiosa), the causative agent of Pierce’s disease (PD), Vitis vinifera cv ‘Thompson Seedless’ (TS) plants were transformed via Agrobacterium tumefaciens with the pear polygalacturonase inhibiting protein (pPGIP). We have previously shown that decreased leaf symptoms and movement of X. fastidiosa was achieved in transgenic plants expressing pPGIP after infection of X. fastidiosa, as in earlier findings with Botrytis cinerea. Microarray analysis (Affymetrix) of leaf tissues of grapevines expressing pPGIP revealed 3007 genes with significantly altered expression (FDR adjusted p
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
Page 9 and 10: Poster Session 2 1:30 PM-3:00 PM Po
Page 11 and 12: P-105 Pathogen responsiveness and v
Page 13 and 14: Table of Contents Keynote Presentat
Page 15 and 16: K-2 Progress in grapevine breeding
Page 17 and 18: K-4 Genetics and Genomics of Grapev
Page 19 and 20: O-2 Additional homologs of the Ren1
Page 21 and 22: O-4 Y. Xu and Y.Wang* Introduction
Page 23 and 24: O-5 Identification of an R2R3 MYB t
Page 25 and 26: O-7 Sequencing of the phylloxera re
Page 27 and 28: O-9 Dissection of defense pathways
Page 29 and 30: O-11 Recent trends and technologies
Page 31 and 32: O-13 Brazilian Grape Breeding Progr
Page 33 and 34: O-15 Development of rootstocks for
Page 35 and 36: O-17 L. Wang* 1 , S. Li 1, 2 , P. F
Page 37 and 38: O-19 Integrated analysis of transcr
Page 39 and 40: O-20 Deciphering the chromosome str
Page 41 and 42: O-22 Identification of table grapes
Page 43 and 44: O-24 Metagenomic sequencing as a to
Page 45 and 46: O-26 Regulation of bud fruitfulness
Page 47 and 48: O-28 Haplotype structure and cluste
Page 49 and 50: O-30 Annette Nassuth Function of Vi
Page 51 and 52: 10 to 25% of the chlorotic symptoms
Page 53 and 54: O-34 Cloning and characterization o
Page 55 and 56: O-36 New insight into the genetics
Page 57 and 58: O-38 Co-Localization of QTLs for Se
Page 59: O-40 Quantitative analysis of textu
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
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P-36 Looking for genetic polymorphi
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P-38 Characterization of a new horm
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P-40 Towards a characterization of
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the overall identified polymorphism
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P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
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P-48 ‘Cioutat’: a somatic varia
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P-50 Estimation of protein spot var
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glucosyltransferase, anthocyanidin
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P-53 Marker-free transgenic grapevi
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P-55 Agrobacterium rhizogenes-media
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P-57 Phenotypic evaluation of ‘Th
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P-59 Phenotypic divergence among gr
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P-61 Grepevine variety determinatio
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agricultural context. This network
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P-64 Genetic structure in a large r
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P-66 Analysis of grape rootstocks b
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P-68 Italian wild grapevine: a stat
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Rebula-Robola group were genotyped
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P-71 Viticultural performance of so
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P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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