conference schedule and program with abstracts - Horticulture ...

P-82
Polyploid induction of mutation by using colchicines on tube seedlings of Victoria grape
Y.-Y. Chang 1 , X. Ji 1 , J.-I. Zhu 2 *, Y. Hao 3
1 College of Agronomy Science, Gansu Agricultural University, Lanzhou, China; 2 College of
Grassland Science, Gansu Agricultural University, Lanzhou, China; 3 Institute of Fruit and
Floriculture Research,Gansu Academy of Agricultural Sciences, Lanzhou, China
*Corresponding author: zhujl@gsau.edu.cn
The unigemmius stem-segment of the tube seedlings of Victoria grape were soaked 24 hours and
48 hours by using colchicines aqueous solution of 0.05% and 0.1% concentration. The results
showed that the best way for reduplicative efficiency was the third and fourth buds treated for 48
hours in 0.05% colchicine and 24 hours in 0.1% colchicine, the doubling rates of primary
generation cells were 33% and 31% respectively; The polyploid cell rate could increase about
10% by subcultured the chimeras a generation. The polyploid cell rate could reach 75.8%-96.6 %
by subcultured the chimeras four to six generations. Through root tip cell chromosome
observation that it was a little effect to the division cycle of root-tip cells in terms of the tube
seedlings with or without illumination in short term., and the optimal sampling time of
chromosome for observation was at 9:30 am.
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