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P-3 Identification of genes related to stenospermocarpy in table grape (Vitis vinifera L.) X.C. Iommi* 1,2 , G. Ravest 1 , M. Guerrero 1 , B. Soto 1 , M. González-Agüero 1-3 , N. Mejia 1 , C. Muñoz 1 , P. Hinrichsen 1 1 Instituto de Investigaciones Agropecuarias. INIA. La Platina Exp Centre. Santiago, Chile; 2 Universidad Andrés Bello. Santiago, Chile; 3 The Plant Cell Biotechnology Millenium Nucleus (PCB-MN), Santiago, Chile Corresponding author: xcasanueva@gmail.com The stenoespermocarpy is a common phenotype in some table grapes and is characterized by the presence of seminal traces instead of complete formed seeds. This trait is highly demanded in international markets and therefore it is in the best interest of many breeders to create new seedless varieties. Genetic mapping analyses have identified a major QTL (quantitative trait loci) responsible for over 50% of the stenoespermocarpic phenotype. This QTL is located in linkage group 18, where the molecular marker VMC7F2 co-localize with VvAGL11, a MADS-box transcription factor related to ovule development. Our aim is to identify additional candidate genes involved in the stenoespermocarpy by combining phenotypic analysis, QTL mapping, transcriptomic analysis and histological studies. QTL mapping was based on an extensive phenotypic characterization using a reference population from a ‘Ruby Seedless' x 'Sultanina' crossing, during 3 growing seasons. The results were partially comparable with the QTLs identified in the same or other populations evaluated.A relevant QTL for seed and berry size was mapped on linkage group 18, where the candidate gene VvAGL11 had been previously mapped. Expression analysis of this gene through berry development during two seasons, showed an increase in its expression after fruit set in seeded segregants, in contrast to the seedless ones. We also have found two other QTLs in linkage groups 2 and 8, explaining 20% of the phenotype. These QTLs have been stable during 3 seasons and we are currently saturating them with newly developed SSRs and AFLP markers to map the relevant genes. A massive sequencing (RNA-Seq) will help to give additional support to the information obtained through positional cloning. In addition, preliminary transcriptomic analyses have identified a number of genes differentially expressed when comparing segregants exhibiting extreme phenotypes for seed size. We are further characterizing the stenospermocarpic phenotype by histological analysis, which shows a defect of the ovule outer integument in seedless segregants. We will use histochemistry to further characterize all our candidate genes. 79 
P-4 Gene-assisted selection for table grape breeding: validation of a molecular marker for seedlessness N. Mejía*, C. Uquillas, J. Paillamanque, J. Ibarra, C. Muñoz, P. Hinrichsen INIA, La Platina Experimental Station, Santiago, Chile *Corresponding author: nmejia@inia.cl Within the scope of the Chilean Table Grape Breeding Program conducted by the Chilean Institute for Agricultural Research (INIA), a major candidate gene (VvAGL11) for seedlessness was identified in chromosome 18. This major QTL was characterized at sequence, transcriptional and genetic level in an experimental progeny derived from the cross of ʻThompson Seedlessʼ x ʻRuby Seedlessʼ. The identified marker explains up to 70% of phenotypic variation and had a perfect allele-phenotype association in the experimental progeny. Also, genetic experiments revealed a partial dominant behavior of the seedless allele over its seeded counterpart. In the present work we report on the validation experiments for this intragenic marker (VvAGL11). Association analysis was performed with a population of 14 different progenies derived from ten common seedless parental genotypes. PCR-genotyping and capillary electrophoresis were performed in these seedlings and parental genotypes to obtain genetic profiles. Phenotyping was done by weighing the seed of 150 berries selected randomly from 3 bunches. The seedless allele was in the heterozygous state in all parental genotypes. A total of six different alleles were identified within the analyzed progenies and up to 11 different genotypes were identified. Association analysis revealed that the intragenic marker (VvAGL11) for seedlessness can be routinely used for assisted selection, both in parents and offspring. The dominant nature of the seedless allele over most of their seeded counterpart was also confirmed in these progenies. The developed marker has the potential to increase the efficiency and efficacy in the selection process. Furthermore, the dominant effect of the seedless allele allows for the selection of parents that can generate large seedless progenies, without the imperative need of crossing two seedless varieties and the subsequent in vitro embryo rescue. 80 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
Page 27 and 28: O-9 Dissection of defense pathways
Page 29 and 30: O-11 Recent trends and technologies
Page 31 and 32: O-13 Brazilian Grape Breeding Progr
Page 33 and 34: O-15 Development of rootstocks for
Page 35 and 36: O-17 L. Wang* 1 , S. Li 1, 2 , P. F
Page 37 and 38: O-19 Integrated analysis of transcr
Page 39 and 40: O-20 Deciphering the chromosome str
Page 41 and 42: O-22 Identification of table grapes
Page 43 and 44: O-24 Metagenomic sequencing as a to
Page 45 and 46: O-26 Regulation of bud fruitfulness
Page 47 and 48: O-28 Haplotype structure and cluste
Page 49 and 50: O-30 Annette Nassuth Function of Vi
Page 51 and 52: 10 to 25% of the chlorotic symptoms
Page 53 and 54: O-34 Cloning and characterization o
Page 55 and 56: O-36 New insight into the genetics
Page 57 and 58: O-38 Co-Localization of QTLs for Se
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77: P-2 PMEI and SPY: Two genes with po
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117 and 118: the overall identified polymorphism
Page 119 and 120: P-43 Proteomic characterization of
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
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glucosyltransferase, anthocyanidin
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P-53 Marker-free transgenic grapevi
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P-55 Agrobacterium rhizogenes-media
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P-57 Phenotypic evaluation of ‘Th
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P-59 Phenotypic divergence among gr
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P-61 Grepevine variety determinatio
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agricultural context. This network
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P-64 Genetic structure in a large r
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P-66 Analysis of grape rootstocks b
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P-68 Italian wild grapevine: a stat
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Rebula-Robola group were genotyped
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P-71 Viticultural performance of so
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P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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