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O-43 Genetic characterization of the USDA grape germplasm collection: Challenges and lessons learned S. Myles* 1 , and E. Buckler 1,2 1 Institute for Genomic Diversity, Cornell University, Ithaca, New York, USA; 2 United States Department of Agriculture – Agricultural Research Service, Ithaca, New York, USA Corresponding author: smm367@cornell.edu Germplasm collections around the world contain an enormous amount of potentially useful genetic variation that is only occasionally quantified and remains largely unexploited. The first step towards harnessing the benefits of these collections involves a thorough survey of genetic variation. Here we describe the use of modern DNA sequencing and genotyping technologies to provide a detailed assessment of the grape germplasm collection of the United States Department of Agriculture (USDA). We show that a custom genotyping array, the Vitis9kSNP array, is useful for quantifying relationships among cultivars within V. vinifera and for distinguishing between highly diverse wild Vitis species from around the world. In addition, with 6000 reliable genetic markers, the resulting genetic data can be used to accurately estimate pedigree relationships among cultivars; to identify regions of the genome that have experienced strong positive selection during domestication and breeding; and to identify genotype-phenotype associations. Accurately assaying genetic variation across an entire genus presents distinct challenges, especially in highly diverse plant species like the grapevine. We discuss these challenges and suggest that modern DNA sequencing technologies will enable us to overcome many if not all of them. 63 
O-44 The Turkish grape (Vitis vinifera L.) SSR database A. Ergül 1 *, Y. Boz 2 , G. Söylemezoğlu 3 , K. Kazan 4 , H. Çelik 3 , M. Bakır 1 , P. Çelikkol 1 , N. Yıldırım 1 , C. Yüksel 1 , A.S. Yaşasın 2 , C. Özer 2 1 Ankara University, Biotechnology Institute, Ankara, Turkey; 2 Ministry of Agriculture and Rural Affairs, Institute of Viticulture, Tekirdağ, Turkey; 3 Ankara University, Faculty of Agriculture, Department of Horticulture, Ankara, Turkey; 4 Commonwealth Scientific and Industrial Research Organization (CSIRO) Plant Industry, Queensland Bioscience Precinct, St. Lucia, Queensland, Australia   *Corresponding author: ergul@agri.ankara.edu.tr Turkey has rich grapevine (Vitis vinifera L.) germplasm, possibly owing to the fact that Anatolia (Asian part of Turkey) is considered as a likely center of cultivated grapevine (McGovern, 2003) and wild grape diversity (Arroya-Garcia et al., 2006 ). Recently, in an attempt to preserve grape genetic resources, a grape germplasm repository called the "National Grapevine Germplasm Vineyard” has been established at the Institute of Viticulture in Tekirdağ, Turkey. This collection currently contains approximately 1150 grapevine accessions collected from different geographical regions over more than 30 years (Çelik 2000). Despite some ampelographic studies conducted on this collection, to date very few studies have been carried out for genetic characterization of this grape germplasm at the molecular level. A project (Project number: 105 G 078) has been started with the support of the Scientific and Technical Research Council of Turkey (TUBITAK-KAMAG) and the Turkish Ministry of Agriculture and Rural Affairs for the genetic characterization of this collection and development of “The Turkish Grape (Vitis vinifera L.) SSR Database”. By the end of the project, genetic identification of the 1115 grape cultivars will have been performed with 21 SSR (Simple Sequence Repeats) loci. Within the scope of the study, genetic parameters (allele size, allele number, heterozygosity rate etc.) of grape cultivars have been identified on the basis of national and regional levels. The number of homonym grapes was detected as 143, while the number of synonym and identical grapes was 178. Out of a total of 1115 grapes analyzed, 854 cultivars were identified apart from the synonym, homonym and identical cases. Studies on the development of the associated database are currently ongoing. The data reported here can be directly compared with other studies in other countries that used these SSR markers on grape. 64 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
Page 11 and 12: P-105 Pathogen responsiveness and v
Page 13 and 14: Table of Contents Keynote Presentat
Page 15 and 16: K-2 Progress in grapevine breeding
Page 17 and 18: K-4 Genetics and Genomics of Grapev
Page 19 and 20: O-2 Additional homologs of the Ren1
Page 21 and 22: O-4 Y. Xu and Y.Wang* Introduction
Page 23 and 24: O-5 Identification of an R2R3 MYB t
Page 25 and 26: O-7 Sequencing of the phylloxera re
Page 27 and 28: O-9 Dissection of defense pathways
Page 29 and 30: O-11 Recent trends and technologies
Page 31 and 32: O-13 Brazilian Grape Breeding Progr
Page 33 and 34: O-15 Development of rootstocks for
Page 35 and 36: O-17 L. Wang* 1 , S. Li 1, 2 , P. F
Page 37 and 38: O-19 Integrated analysis of transcr
Page 39 and 40: O-20 Deciphering the chromosome str
Page 41 and 42: O-22 Identification of table grapes
Page 43 and 44: O-24 Metagenomic sequencing as a to
Page 45 and 46: O-26 Regulation of bud fruitfulness
Page 47 and 48: O-28 Haplotype structure and cluste
Page 49 and 50: O-30 Annette Nassuth Function of Vi
Page 51 and 52: 10 to 25% of the chlorotic symptoms
Page 53 and 54: O-34 Cloning and characterization o
Page 55 and 56: O-36 New insight into the genetics
Page 57 and 58: O-38 Co-Localization of QTLs for Se
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61: O-42 Transcriptional changes in res
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
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P-38 Characterization of a new horm
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P-40 Towards a characterization of
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the overall identified polymorphism
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P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
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P-48 ‘Cioutat’: a somatic varia
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P-50 Estimation of protein spot var
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glucosyltransferase, anthocyanidin
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P-53 Marker-free transgenic grapevi
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P-55 Agrobacterium rhizogenes-media
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P-57 Phenotypic evaluation of ‘Th
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P-59 Phenotypic divergence among gr
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P-61 Grepevine variety determinatio
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agricultural context. This network
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P-64 Genetic structure in a large r
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P-66 Analysis of grape rootstocks b
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P-68 Italian wild grapevine: a stat
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Rebula-Robola group were genotyped
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P-71 Viticultural performance of so
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P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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