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O-37 Differential expressed genes in berries of cv. Sangiovese (Vitis Vinifera L.) during ripening following cluster thinning at véraison C. Pastore* 1 , I. Filippetti 1 , S. Zenoni 2 , M. Fasoli 2 , A. Ferrarini 3 , M. Pezzotti 2 , C. Intrieri 1 1 Department of Fruit Tree and Woody Plant Science, University of Bologna, Bologna, Italy; 2 Department of Sciences, Technologies and Grapevine and Wine Markets, University of Verona, San Floriano, Verona, Italy; 3 Department of Biotechnology, University of Verona, Verona, Italy *Corresponding author: cpastore@agrsci.unibo.it Yield reduction and quality improvement are not always strictly related, cluster thinning is considered a technique which, involving an increase in source-sink ratio, could lead to improvement in grape berry sugar and anthocyanin concentration. In this research an integrated agronomical, biochemical and molecular approach aims to understand which mechanisms are involved, during ripening, in determining final berry composition in cv Sangiovese vines submitted to thinning at véraison (CT, 50% of total clusters removed at véraison). The yield reduction observed in CT vines implied a significant increase in leaf area/crop weight ratio, which caused an acceleration in berry ripening rate immediately after the treatment and higher values of soluble sugars at harvest compared to control vines (C). Anthocyanin amount was strongly affected by the treatment, as their accumulation increases in parallel with soluble sugars content, reaching higher values than C at harvest. Microarray analyses, carried out with a NimbleGen array assembled on the basis of the 12X Vitis vinifera genome sequence, allowed to discriminate between CT and C at the end of véraison (14% of 29550 genes analyzed were differentially expressed between CT and C at this stage). Genes involved in carbohydrate metabolism, response to stress and flavonoid accumulation were analyzed in depth. As a result, CT transcriptional profile was characterized by the increase in expression of genes involved in sugars storage which usually follows sugar utilization as energy source, and by a decrease in genes induced in response to oxidative stress, corresponding to the final ripening phase. Source/sink ratio increase, positively influenced anthocyanin structural and transcriptional regulatory genes and in particular those involved in anthocyanin transport, which were strongly up-regulated in CT compared to C. The results of this research confirm the key role of source/sink ratio in conditioning sugars metabolism and reveals that carbohydrates availability is a crucial issue in triggering expression of genes involved in anthocyanin biosynthesis in field condition too. 57 
O-38 Co-Localization of QTLs for Seedlessness and Downy Mildew Resistance in Grapevine L.F. Revers* 1 , D. Costenaro da Silva 1 , V.S. Lampe 1 , P.R.D. Oliveira 1 , L.R. Garrido 1 , L.J. Welter 2 , P.B. Irala 3 1 Laboratory of Plant Molecular Biology, Embrapa Uva e Vinho, Bento Gonçalves, Rio Grande do Sul, Brazil; 2 Universidade Federal do Pampa, Department of Plant Breeding and Genetics, Itaqui, Rio Grande do Sul, Brazil; 3 ProfiGen do Brasil Ltda, Santa Cruz do Sul, Rio Grande do Sul, Brazil *Corresponding author: luis@cnpuv.embrapa.br A genetic linkage map of grapevine was constructed using a pseudo-testcross strategy based on a cross between the seedless Vitis vinifera ‘Crimson Seedless’ and the complex hybrid ‘Villard Blanc’ (Seyve Villard 12-375), resistant to downy mildew. A total of 316 DNA markers, including 262 AFLP, 48 single sequence repeats (SSR), 2 SCARs (sequence characterized amplified region) and 4 minisatellites markers were used to generate a map for each parent. For both parents, 19 linkage groups were obtained, covering 1111 cM and 926 cM for ‘Villard Blanc’ and ‘Crimson Seedless’, respectively. The position of SSR loci in the obtained maps is consistent with the genomic sequence. Quantitative traits loci (QTLs) for seedlessness and resistance to Plasmopara viticola (downy mildew) were investigated. Two major effect QTLs for downy mildew resistance and seedlessness were mapped on the same region of the linkage group 18. These QTLs explain 25,0-55,7% and 54,0-62,4% of total variance, respectively. The MIKC C - Type MADS box gene VvAG3; whose ortholog is involved in Arabidopsis carpel and ovule development, and located in the confidence interval of the seedlessness QTL detected on the LG 18, could be considered as a candidate gene to control seed development in grape. Colocalizations were found in the same region, between the position of the Rpv3 locus, which is very rich in TIR-NBS-LRR resistant gene analogs, and the main QTL identified for downy mildew resistance. Our results demonstrate that the same region of LG 18 contains important genetic determinants for seedlessness and downy mildew resistance in grapevine. Moreover, assessing the allelic variation at these agronomically important loci provides a basis for the development of marker-assisted selection for seedlessness and downy mildew simultaneously. 58 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
Page 5 and 6: Topic: Adaptation to soils and clim
Page 7 and 8: Tuesday August 3, 2010 Oral Session
Page 9 and 10: Poster Session 2 1:30 PM-3:00 PM Po
Page 11 and 12: P-105 Pathogen responsiveness and v
Page 13 and 14: Table of Contents Keynote Presentat
Page 15 and 16: K-2 Progress in grapevine breeding
Page 17 and 18: K-4 Genetics and Genomics of Grapev
Page 19 and 20: O-2 Additional homologs of the Ren1
Page 21 and 22: O-4 Y. Xu and Y.Wang* Introduction
Page 23 and 24: O-5 Identification of an R2R3 MYB t
Page 25 and 26: O-7 Sequencing of the phylloxera re
Page 27 and 28: O-9 Dissection of defense pathways
Page 29 and 30: O-11 Recent trends and technologies
Page 31 and 32: O-13 Brazilian Grape Breeding Progr
Page 33 and 34: O-15 Development of rootstocks for
Page 35 and 36: O-17 L. Wang* 1 , S. Li 1, 2 , P. F
Page 37 and 38: O-19 Integrated analysis of transcr
Page 39 and 40: O-20 Deciphering the chromosome str
Page 41 and 42: O-22 Identification of table grapes
Page 43 and 44: O-24 Metagenomic sequencing as a to
Page 45 and 46: O-26 Regulation of bud fruitfulness
Page 47 and 48: O-28 Haplotype structure and cluste
Page 49 and 50: O-30 Annette Nassuth Function of Vi
Page 51 and 52: 10 to 25% of the chlorotic symptoms
Page 53 and 54: O-34 Cloning and characterization o
Page 55: O-36 New insight into the genetics
Page 59 and 60: O-40 Quantitative analysis of textu
Page 61 and 62: O-42 Transcriptional changes in res
Page 63 and 64: O-44 The Turkish grape (Vitis vinif
Page 65 and 66: Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
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P-32 Vitis vinifera genome annotati
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P-34 Genome-wide identification of
Page 111 and 112:
P-36 Looking for genetic polymorphi
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P-38 Characterization of a new horm
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P-40 Towards a characterization of
Page 117 and 118:
the overall identified polymorphism
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P-43 Proteomic characterization of
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P-45 Preliminary observations on th
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grapevine which will eventually be
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P-48 ‘Cioutat’: a somatic varia
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P-50 Estimation of protein spot var
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glucosyltransferase, anthocyanidin
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P-53 Marker-free transgenic grapevi
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P-55 Agrobacterium rhizogenes-media
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P-57 Phenotypic evaluation of ‘Th
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P-59 Phenotypic divergence among gr
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P-61 Grepevine variety determinatio
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agricultural context. This network
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P-64 Genetic structure in a large r
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P-66 Analysis of grape rootstocks b
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P-68 Italian wild grapevine: a stat
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Rebula-Robola group were genotyped
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P-71 Viticultural performance of so
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P-73    Molecular characterizat
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P-75 Variation of berry polyphenoli
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P-77 Morphological and molecular id
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P-79 Studies on an excellent, early
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P-81 Effectiveness of different cul
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P-83 Some early maturing table grap
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P-85 New triploid seedless table gr
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P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
Page 187 and 188:
P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
Page 191 and 192:
P-112 Evaluation of grapevine germp
Page 193 and 194:
P-114 Evaluation of four new rootst
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