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P-42 Phenotypic plasticity in Vitis vinifera: how environment shapes wine S. Dal Santo*, S. Zenoni, M. Fasoli, P. Tononi, N. Dago, M. Delledonne, M. Pezzotti Department of Biotechnology, University of Verona, Verona, Italy *Corresponding author: silvia.dalsanto@univr.it Phenotypic plasticity can be defined as a re-programming of the genome in response to changes in the environment, resulting in new, different phenotypes. Plastic responses range from morphological modifications to drastic changes in physiology, life history and behavior, depending on the environment the organism faces. Phenotypic plasticity has been deeply studied in plants, both for its agronomical significance as well as its ecological and evolutionary implications. However, the mechanisms determining plastic changes are still largely unknown, especially for plants cultivated in open fields where the simultaneous challenge of different environmental signals leads to complex responses in terms of gene expression, metabolic rearrangements and epigenetic mechanisms. Vitis vinifera spp is one of the most plastic plants known, a single genotype being able to produce berries with different quality, thus different wine qualities, depending on the micro-environment where it is cultivated. Moreover, plastic responses in grapevine are one of the causes of excellent-to-poor wine vintages. In order to better understand dynamics of phenotypic plasticity in Vitis vinifera, we studied the behavior of a single genotype, the clone 48 of the cultivar ‘Corvina’, in different environments. We sampled berries at three developmental stages (veraison, pre-ripening and ripening), during three consecutive years (2006, 2007 and 2008). These were obtained from eleven different vineyards in Valpollicella, Soave and Bardolino, the three most important areas for local wine production in Verona. The transcriptome will be studied using the Nimblegen microarray platform, and differential gene expression will be correlated with central micro-environmental and agricultural features such as the soil type, altitude and orientation of rows, type of breeding, age of vineyards and type of rootstock. Furthermore, transcriptomic as well as metabolomic data will be analyzed in light of the chemical-physical characteristics of wines produced in the aforementioned vineyards, in an attempt to create a direct link between grapevine plasticity norms and the multitude of wine quality facets. 119 
P-43 Proteomic characterization of downy mildew-infected grapevine plants D. Cecconi, A. Milli, L. Bortesi, A. Persi, A. Lovato, M. Pezzotti, A. Polverari* Dipartimento di Biotecnologie, Università di Verona, Italy *Corresponding author: annalisa.polverari@univr.it We have carried out a proteomic 2D analysis of Vitis vinifera leaves cv. Pinot Noir artificially infected with Plasmopara viticola (30000 sporangia per ml), and collected at 24, 48 and 96 hours post-inoculation. Leaf tissues from 3 independent biological replicates were pooled. After testing different protocols for protein extraction, good quality 2D maps were obtained by performing protein extraction in a solubilizing/lysing solution containing 7M urea, 2M thiourea, 80mM citric acid, 1% C7Bz0 and 1x protease inhibitor cocktail. Gels (five per each sample) were scanned using a Bio-Rad VersaDoc 1000 imaging system and analyzed by PDQuest software (Bio-Rad) for spot detection, background subtraction and spot OD intensity quantification. After normalization and statistical analysis, about 100 spots differentially expressed were identified (fold-change ≥ 2, with p≤0.05). Identification by RP-HPLC-ESI- MS/MS analysis, revealed 72 unique proteins, 56 up-regulated and 16 down-regulated. Functional categories prevalently affected by infection were related to Calvin cycle and glycolysis (28%), oxidative stress response (19%), defence response (12%), photosynthesis (9%) and protein folding (6%). A peculiar pattern in the number of modulated proteins over time was observed: 53 and 47 differentially expressed proteins were detected at 24 hpi and 96 hpi, respectively, and only 14 at 48 hpi, none of which included in the “defence response” category, suggesting a temporary breakdown of the response, that will be the object of further investigations. 120 
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Date Time Session Topic Sunday Augu
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Monday August 2, 2010 Opening Plena
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Topic: Adaptation to soils and clim
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Tuesday August 3, 2010 Oral Session
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Poster Session 2 1:30 PM-3:00 PM Po
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P-105 Pathogen responsiveness and v
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Table of Contents Keynote Presentat
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K-2 Progress in grapevine breeding
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K-4 Genetics and Genomics of Grapev
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O-2 Additional homologs of the Ren1
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O-4 Y. Xu and Y.Wang* Introduction
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O-5 Identification of an R2R3 MYB t
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O-7 Sequencing of the phylloxera re
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O-9 Dissection of defense pathways
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O-11 Recent trends and technologies
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O-13 Brazilian Grape Breeding Progr
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O-15 Development of rootstocks for
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O-17 L. Wang* 1 , S. Li 1, 2 , P. F
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O-19 Integrated analysis of transcr
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O-20 Deciphering the chromosome str
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O-22 Identification of table grapes
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O-24 Metagenomic sequencing as a to
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O-26 Regulation of bud fruitfulness
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O-28 Haplotype structure and cluste
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O-30 Annette Nassuth Function of Vi
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10 to 25% of the chlorotic symptoms
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O-34 Cloning and characterization o
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O-36 New insight into the genetics
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O-38 Co-Localization of QTLs for Se
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O-40 Quantitative analysis of textu
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O-42 Transcriptional changes in res
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O-44 The Turkish grape (Vitis vinif
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Association genetics in relation wi
Page 67 and 68: O-47 Intravarietal variability of C
Page 69 and 70: O-49 Molecular survey of Georgian t
Page 71 and 72: O-51 Marker-assisted breeding: Iden
Page 73 and 74: O-53 An Integrated approach to stud
Page 75 and 76: O-55 Characteristics of promising m
Page 77 and 78: P-2 PMEI and SPY: Two genes with po
Page 79 and 80: P-4 Gene-assisted selection for tab
Page 81 and 82: P-6 Polyphenolic potential of the r
Page 83 and 84: P-8 Monoterpene levels in different
Page 85 and 86: P-10 Usefulness of the SCC8 scar ma
Page 87 and 88: P-12 The onset of berry ripening is
Page 89 and 90: P-14 Morphological characteristics
Page 91 and 92: P-16 A genetic analysis of berry qu
Page 93 and 94: P-18 Use of molecular markers for s
Page 95 and 96: P-20 Characterization of key compon
Page 97 and 98: P-22 Expression of early light-indu
Page 99 and 100: P-24 The development of molecular m
Page 101 and 102: P-26 Identification of ABA inducibl
Page 103 and 104: P-28 In vitro selection of HYP tole
Page 105 and 106: P-30 In silico SNP detection for ge
Page 107 and 108: P-32 Vitis vinifera genome annotati
Page 109 and 110: P-34 Genome-wide identification of
Page 111 and 112: P-36 Looking for genetic polymorphi
Page 113 and 114: P-38 Characterization of a new horm
Page 115 and 116: P-40 Towards a characterization of
Page 117: the overall identified polymorphism
Page 121 and 122: P-45 Preliminary observations on th
Page 123 and 124: grapevine which will eventually be
Page 125 and 126: P-48 ‘Cioutat’: a somatic varia
Page 127 and 128: P-50 Estimation of protein spot var
Page 129 and 130: glucosyltransferase, anthocyanidin
Page 131 and 132: P-53 Marker-free transgenic grapevi
Page 133 and 134: P-55 Agrobacterium rhizogenes-media
Page 135 and 136: P-57 Phenotypic evaluation of ‘Th
Page 137 and 138: P-59 Phenotypic divergence among gr
Page 139 and 140: P-61 Grepevine variety determinatio
Page 141 and 142: agricultural context. This network
Page 143 and 144: P-64 Genetic structure in a large r
Page 145 and 146: P-66 Analysis of grape rootstocks b
Page 147 and 148: P-68 Italian wild grapevine: a stat
Page 149 and 150: Rebula-Robola group were genotyped
Page 151 and 152: P-71 Viticultural performance of so
Page 153 and 154: P-73    Molecular characterizat
Page 155 and 156: P-75 Variation of berry polyphenoli
Page 157 and 158: P-77 Morphological and molecular id
Page 159 and 160: P-79 Studies on an excellent, early
Page 161 and 162: P-81 Effectiveness of different cul
Page 163 and 164: P-83 Some early maturing table grap
Page 165 and 166: P-85 New triploid seedless table gr
Page 167 and 168: P-87 Grape breeding and viticulture
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P-89 The usefulness of allotetraplo
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P-91 Drying rate of fresh berries f
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P-93 Development of table and raisi
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P-95 Reaction of grape rootstocks t
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P-97 Genetic analysis of the resist
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P-99 Berry cracking caused powdery
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P-101 Elicitation of grapevine defe
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P-103 The biological characteristic
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P-105 Pathogen responsiveness and v
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P-107 Screening grape hybrid famili
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P-109 Characterization of an ankyri
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P-112 Evaluation of grapevine germp
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P-114 Evaluation of four new rootst
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