Pleosporales - CBS - KNAW

Fungal Diversity
Concluding remarks
The only morphological difference between M. velataspora
and M. ramunculicola are their morphology of
ascomata and size of ascospores (Hyde 1991b). But M.
velataspora was reported staining the woody substrate (or
agar in culture) purple (Hyde and Borse 1986; Hyde
1991b). Although this character could not be verified in
the strain used by Suetrong et al. (2009), purple staining has
been reported to have phylogenetic significance at familial
rank in freshwater fungi (Zhang et al. 2009a).
Murispora Yin. Zhang, C.L. Schoch, J. Fourn., Crous & K.
D. Hyde, Stud. Mycol. 64: 95 (2009b). (Amniculicolaceae)
Generic description
Habitat freshwater, saprobic. Ascomata medium-sized,
scattered to gregarious, immersed, lenticular, apex slightly
protruding, opening through a small rounded pore,
substrate stained purple. Peridium thin, composed of a
few layers cells of textura angularis, thicker at the apex
with pseudoparenchymatous cells. Hamathecium of narrowly
cellular pseudoparaphyses, embedded in mucilage.
Asci 8-spored, bitunicate, fissitunicate, biseriate, cylindroclavate
with short pedicels. Ascospores curved- fusoid
with narrowly rounded ends, golden yellow turning brown
when senescent, multi-septate, constricted at the septa,
with one, rarely two longitudinal septa in all cells except
end cells, smooth or finely verruculose, surrounded by a
wide mucilaginous sheath.
Anamorphs reported for genus: Phoma (Webster 1957).
Literature: Zhang et al. 2009a, b.
Type species
Murispora rubicunda (Niessl) Yin. Zhang, J. Fourn. & K.
D. Hyde, Stud. Mycol. 64: 96 (2009a). (Fig. 64)
≡ Pleospora rubicunda Niessl, Notiz. Pyr.: 31 (1876).
Ascomata 170–200 μm high×380–410 μm diam.,
scattered to gregarious, immersed, lenticular, apex
laterally flattened, black, slightly protruding, opening
through a small rounded pore, substrate stained purple
(Fig. 64a). Peridium 15–18 μm thick at sides, composed
of 3–4 layers cells of textura angularis, upto28–30 μm
thick at the apex with very thick-walled cells, pseudoparenchymatous,
nearly absent at the base (Fig. 64b).
Hamathecium of narrowly cellular pseudoparaphyses,
1–1.7 μm broad, embedded in mucilage. Asci 124–
142×19–21 μm, 8-spored, bitunicate, fissitunicate,
biseriate, cylindro-clavate with a small ocular chamber,
with short pedicels (Fig. 64c). Ascospores 30–38×10–
12 μm, curved-fusoid with narrowly rounded ends,
golden yellow turning brown when senescent, 7–9 transversally
septate, constricted at the septa, with one, rarely
two longitudinal septa in all cells except end cells which
are often slightly paler, all cells filled with a large
refractive guttule, smooth to finely verruculose, surrounded
by a wide mucilaginous sheath (Fig. 64d).
Anamorph: Phoma sp. (Webster 1957).
Material examined: FRANCE, Haute Garonne,
Avignonet, Lac de Rosel, 16 Jan. 2007, on submerged
dead herbaceous stem (Dipsacus?), leg. Michel Delpont,
det. Jacques Fournier (IFRD 2017).
Notes
Morphology
Murispora was introduced based on Pleospora rubicunda
which is characterized by immersed, erumpent or
nearly superficial, globose to subglobose, elongated weakly
papillate ascomata which stain the woody substrate purple,
trabeculate pseudoparaphyses, 8-spored, bitunicate, fissitunicate,
oblong to clavate asci, fusoid, pale or reddish
brown, muriform ascospores (Zhang et al. 2009a). A
phylogenetic study indicated that Murispora forms a robust
clade with species of Amniculicola, and Amniculicolaceae
was introduced to accommodate them (Zhang et al. 2009a).
Phylogenetic study
Murispora rubicunda forms a robust clade with species of
Amniculicola and Neophaeosphaeria (Zhang et al. 2009a).
Concluding remarks
As has mentioned by Eriksson (1981, P. 135), the purplestaining
species of Pleospora, treated by Webster (1957),
should not belong to the Pleosporaceae. Both Pleospora
straminis and P. rubelloides should be closely related to
Murispora.
Neomassariosphaeria Yin. Zhang, J. Fourn. & K.D. Hyde,
Stud. Mycol. 64: 96 (2009a). (Amniculicolaceae)
Generic description
Habitat freshwater, saprobic. Ascomata medium-sized,
scattered or in small groups, immersed, with a slightly
protruding elongated papilla, ostiolate, lenticular, stain the
substrate purple. Peridium thin. Hamathecium of dense,
long cellular pseudoparaphyses, septate. Asci 8-spored,
bitunicate, fissitunicate, cylindro-clavate, with short furcate
pedicels. Ascospores 2-3-seriate, narrowly fusoid, somewhat
curved, reddish brown, multi-septate, slightly constricted
at the primary septum.
Anamorphs reported for genus: none.
Literature: Leuchtmann 1984; Zhang et al. 2009a, b.